MFAP1_DROME
ID MFAP1_DROME Reviewed; 478 AA.
AC Q9W062;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Microfibrillar-associated protein 1 {ECO:0000250|UniProtKB:P55081};
DE AltName: Full=Spliceosome B complex protein MFAP1 {ECO:0000250|UniProtKB:P55081};
GN Name=Mfap1 {ECO:0000312|FlyBase:FBgn0035294};
GN ORFNames=CG1017 {ECO:0000312|FlyBase:FBgn0035294};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK77294.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK77294.1};
RC TISSUE=Head {ECO:0000312|EMBL:AAK77294.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBUNIT, INTERACTION WITH PRP38, SUBCELLULAR LOCATION, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18765666; DOI=10.1074/jbc.m803512200;
RA Andersen D.S., Tapon N.;
RT "Drosophila MFAP1 is required for pre-mRNA processing and G2/M
RT progression.";
RL J. Biol. Chem. 283:31256-31267(2008).
CC -!- FUNCTION: Required for pre-mRNA splicing.
CC {ECO:0000269|PubMed:18765666}.
CC -!- SUBUNIT: Component of the spliceosome B complex (By similarity).
CC Interacts (via C-terminus) with Prp38. {ECO:0000250|UniProtKB:P55081,
CC ECO:0000269|PubMed:18765666}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:18765666}.
CC -!- SIMILARITY: Belongs to the MFAP1 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF47596.1; -; Genomic_DNA.
DR EMBL; AY047562; AAK77294.1; -; mRNA.
DR RefSeq; NP_647679.1; NM_139422.3.
DR AlphaFoldDB; Q9W062; -.
DR SMR; Q9W062; -.
DR IntAct; Q9W062; 5.
DR STRING; 7227.FBpp0072720; -.
DR PaxDb; Q9W062; -.
DR PRIDE; Q9W062; -.
DR DNASU; 38256; -.
DR EnsemblMetazoa; FBtr0072841; FBpp0072720; FBgn0035294.
DR GeneID; 38256; -.
DR KEGG; dme:Dmel_CG1017; -.
DR UCSC; CG1017-RA; d. melanogaster.
DR CTD; 4236; -.
DR FlyBase; FBgn0035294; Mfap1.
DR VEuPathDB; VectorBase:FBgn0035294; -.
DR eggNOG; KOG1425; Eukaryota.
DR GeneTree; ENSGT00690000102225; -.
DR HOGENOM; CLU_023077_1_0_1; -.
DR InParanoid; Q9W062; -.
DR OMA; FHNERAG; -.
DR OrthoDB; 1535406at2759; -.
DR PhylomeDB; Q9W062; -.
DR BioGRID-ORCS; 38256; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38256; -.
DR PRO; PR:Q9W062; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035294; Expressed in oviduct (Drosophila) and 25 other tissues.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0032991; C:protein-containing complex; IPI:FlyBase.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:FlyBase.
DR GO; GO:0010389; P:regulation of G2/M transition of mitotic cell cycle; IMP:FlyBase.
DR InterPro; IPR033194; MFAP1.
DR InterPro; IPR009730; MFAP1_C.
DR PANTHER; PTHR15327; PTHR15327; 1.
DR Pfam; PF06991; MFAP1; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT CHAIN 1..478
FT /note="Microfibrillar-associated protein 1"
FT /id="PRO_0000438173"
FT REGION 1..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..478
FT /note="Interaction with Prp38"
FT /evidence="ECO:0000269|PubMed:18765666"
FT REGION 456..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..87
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..173
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..216
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..276
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 478 AA; 56135 MW; 5E0139CFE1CD34E8 CRC64;
MSAATAAAAA SGIQSTAGAI PMRNEKGELS MQKVKVQRYI SGKRPDYARA DSSSEESDDD
DFIDTRKRLE RHKAERHKLE LSRQGGSAEG EERAAGEGQE EDDAEVDDPR LRRLRQRPVD
MEDMERERRE RHRHIHEPEI MESDSEDEEE DEGAQGAIQR GTNKITLASE SDTDAELSDT
ELENRRTKLR SRMLQQQREE EVLQKEDEKQ SESSESESSE YEEETESEED NEPRLKPLFV
RKRDRATIQE KEREAQKQKQ LEAEAKRAAK ERRRATLRMV EESVKKDLEK TKPETNEACI
EDVCTDDEND EVEYEAWKLR ELKRMKRDRE ERDNVEREKL DIDRMRNMTE EERRQELRQN
PKVVTNKATK GKYKFLQKYY HRGAFYLDEE NDVLKRDFAQ ATLEDHFDKT ILPKVMQVKN
FGRCGRTKYT HLVDQDTTKF DSPWYAESSS NIKFHNEHAG GMRQQFDKPT GSKRKKME
