ID   MFAP5_BOVIN             Reviewed;         170 AA.
AC   Q28022; Q3ZC93;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Microfibrillar-associated protein 5;
DE            Short=MFAP-5;
DE   AltName: Full=MP25;
DE   AltName: Full=Microfibril-associated glycoprotein 2;
DE            Short=MAGP-2;
DE   Flags: Precursor;
GN   Name=MFAP5; Synonyms=MAGP2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=8557636; DOI=10.1074/jbc.271.2.1096;
RA   Gibson M.A., Hatzinikolas G., Kumaratilake J.S., Sandberg L.B.,
RA   Nicholl J.K., Sutherland G.R., Cleary E.G.;
RT   "Further characterization of proteins associated with elastic fiber
RT   microfibrils including the molecular cloning of MAGP-2 (MP25).";
RL   J. Biol. Chem. 271:1096-1103(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Mammary gland;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INTERACTION WITH FBN1 AND FBN2, AND TISSUE SPECIFICITY.
RX   PubMed=15131124; DOI=10.1074/jbc.m313672200;
RA   Hanssen E., Hew F.H., Moore E., Gibson M.A.;
RT   "MAGP-2 has multiple binding regions on fibrillins and has covalent
RT   periodic association with fibrillin-containing microfibrils.";
RL   J. Biol. Chem. 279:29185-29194(2004).
CC   -!- FUNCTION: May play a role in hematopoiesis. In the cardiovascular
CC       system, could regulate growth factors or participate in cell signaling
CC       in maintaining large vessel integrity (By similarity). Component of the
CC       elastin-associated microfibrils (By similarity).
CC       {ECO:0000250|UniProtKB:Q13361, ECO:0000250|UniProtKB:Q9QZJ6}.
CC   -!- SUBUNIT: Interacts with TGFB2 (By similarity). Interacts with BMP2 (By
CC       similarity). Interacts with FBN1 (via N-terminal domain) and FBN2
CC       (PubMed:15131124). {ECO:0000250|UniProtKB:Q9QZJ6,
CC       ECO:0000269|PubMed:15131124}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix.
CC   -!- TISSUE SPECIFICITY: Associated with fibrillin-containing microfibrils
CC       of the developing nuchal ligament. {ECO:0000269|PubMed:15131124}.
CC   -!- PTM: Forms intermolecular disulfide bonds either with other MAGP-2
CC       molecules or with other components of the microfibrils.
CC   -!- SIMILARITY: Belongs to the MFAP family. {ECO:0000305}.
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DR   EMBL; U37282; AAA96751.1; -; mRNA.
DR   EMBL; BC102769; AAI02770.1; -; mRNA.
DR   RefSeq; NP_776811.1; NM_174386.2.
DR   RefSeq; XP_005207104.1; XM_005207047.1.
DR   AlphaFoldDB; Q28022; -.
DR   STRING; 9913.ENSBTAP00000000398; -.
DR   PaxDb; Q28022; -.
DR   GeneID; 281908; -.
DR   KEGG; bta:281908; -.
DR   CTD; 8076; -.
DR   eggNOG; ENOG502S4DY; Eukaryota.
DR   HOGENOM; CLU_138028_0_0_1; -.
DR   InParanoid; Q28022; -.
DR   OrthoDB; 1427454at2759; -.
DR   TreeFam; TF333418; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0001527; C:microfibril; IDA:UniProtKB.
DR   GO; GO:0060216; P:definitive hemopoiesis; ISS:UniProtKB.
DR   GO; GO:0048048; P:embryonic eye morphogenesis; IBA:GO_Central.
DR   InterPro; IPR008673; MAGP.
DR   PANTHER; PTHR16485; PTHR16485; 1.
DR   Pfam; PF05507; MAGP; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Extracellular matrix;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..170
FT                   /note="Microfibrillar-associated protein 5"
FT                   /id="PRO_0000018684"
FT   MOTIF           30..32
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        76
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        2
FT                   /note="T -> A (in Ref. 2; AAI02770)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   170 AA;  19080 MW;  913A37D3EB8C013A CRC64;
     MTFFGPKVLL LLTALIMSSG RTPLGVSGQR GDDVTTVTSE TFTEDPNLVN DPATDETVLA
     DIQPSTDDLA SLNDKNTTTE CRDEKFACTR LYSVHRPIKQ CLHQICFTSS RRMYVINNEI
     CSRLVCKEHE AMKDELCRQK AGLPPRRLRR SNYFRLPPCD NVNLQGPSGL