ID   MFB1_YEAST              Reviewed;         465 AA.
AC   Q04922; D6VSK2;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Mitochondrial F-box protein MFB1;
GN   Name=MFB1; OrderedLocusNames=YDR219C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 1360 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z48612; CAA88499.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA12062.1; -; Genomic_DNA.
DR   PIR; S59426; S59426.
DR   RefSeq; NP_010505.3; NM_001180527.3.
DR   AlphaFoldDB; Q04922; -.
DR   BioGRID; 32272; 83.
DR   DIP; DIP-6353N; -.
DR   IntAct; Q04922; 7.
DR   MINT; Q04922; -.
DR   STRING; 4932.YDR219C; -.
DR   iPTMnet; Q04922; -.
DR   MaxQB; Q04922; -.
DR   PaxDb; Q04922; -.
DR   PRIDE; Q04922; -.
DR   EnsemblFungi; YDR219C_mRNA; YDR219C; YDR219C.
DR   GeneID; 851805; -.
DR   KEGG; sce:YDR219C; -.
DR   SGD; S000002627; MFB1.
DR   VEuPathDB; FungiDB:YDR219C; -.
DR   eggNOG; ENOG502S5PF; Eukaryota.
DR   HOGENOM; CLU_667626_0_0_1; -.
DR   InParanoid; Q04922; -.
DR   OMA; EIERHYP; -.
DR   BioCyc; YEAST:G3O-29800-MON; -.
DR   PRO; PR:Q04922; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; Q04922; protein.
DR   GO; GO:0031315; C:extrinsic component of mitochondrial outer membrane; IDA:SGD.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; ISA:SGD.
DR   GO; GO:0007005; P:mitochondrion organization; IMP:SGD.
DR   GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; ISA:SGD.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR001810; F-box_dom.
DR   Pfam; PF00646; F-box; 1.
DR   SMART; SM00256; FBOX; 1.
DR   SUPFAM; SSF81383; SSF81383; 1.
DR   PROSITE; PS50181; FBOX; 1.
PE   1: Evidence at protein level;
KW   Mitochondrion; Reference proteome.
FT   CHAIN           1..465
FT                   /note="Mitochondrial F-box protein MFB1"
FT                   /id="PRO_0000253803"
FT   DOMAIN          14..60
FT                   /note="F-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   REGION          253..279
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   465 AA;  53168 MW;  FDF72002778D6FA9 CRC64;
     MTLFSCSVQM PLEERSLTNL PLNLLFRILS HLDMNDLQNI GKTCTLLRML ANENIVYRNA
     VIGSNGNMWW TKNVLVDVFD VLNFNRKAMK TLNSHNISLV ASLRNVQRKY KLGVIDPARK
     TISYRTNEVE SKEKGSVKDL NMDLNEPTEI TREQIAHTAI LQGMNQFIEL NDKAFRTHSA
     DSDDTYIEEN NGEIHSLHGL EKNTTFEEDL VKKPPFIPSP TFSNYSRSST NSVFSSSSPK
     LLDDDWNNIT MDFTKSRDPD YKEMTPTSTE SSDSITRLRK SNKVKDKAEL FEKLIFRDSR
     PLKTKKKDNP RLKLSSSLSA NDEDFRKIIS PPSDILPKVG RRSVSRGYLE EIERHYPDFN
     GETTNPLAIK RVNSTKIANY EQLIIKENSS NCKGITEKND ENKFQRSHTS PVIELSKPHQ
     RSKLKAVVTD GNKICYRKIE LDNPSGSNTN DHVIKRLDAN TDFNI