MFD4A_MOUSE
ID MFD4A_MOUSE Reviewed; 510 AA.
AC Q6PDC8; Q8C3M3; Q8C816; Q8CAF5;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Major facilitator superfamily domain-containing protein 4A;
DE AltName: Full=Major facilitator superfamily domain-containing protein 4;
GN Name=Mfsd4a {ECO:0000312|MGI:MGI:2442786}; Synonyms=Mfsd4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
RC STRAIN=C57BL/6J;
RC TISSUE=Cerebellum, Hypothalamus, Kidney, and Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q6PDC8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PDC8-2; Sequence=VSP_022541, VSP_022543;
CC Name=3;
CC IsoId=Q6PDC8-3; Sequence=VSP_022542;
CC Name=4;
CC IsoId=Q6PDC8-4; Sequence=VSP_022543;
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC30158.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK038882; BAC30158.1; ALT_INIT; mRNA.
DR EMBL; AK048692; BAC33423.1; -; mRNA.
DR EMBL; AK085484; BAC39455.1; -; mRNA.
DR EMBL; AK162495; BAE36946.1; -; mRNA.
DR EMBL; AC107837; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC058790; AAH58790.1; -; mRNA.
DR CCDS; CCDS15280.2; -. [Q6PDC8-4]
DR CCDS; CCDS48357.1; -. [Q6PDC8-1]
DR RefSeq; NP_001108134.1; NM_001114662.1. [Q6PDC8-1]
DR RefSeq; NP_766098.2; NM_172510.4. [Q6PDC8-4]
DR RefSeq; XP_006529407.1; XM_006529344.3. [Q6PDC8-4]
DR RefSeq; XP_006529408.1; XM_006529345.3. [Q6PDC8-2]
DR RefSeq; XP_017175139.1; XM_017319650.1. [Q6PDC8-2]
DR AlphaFoldDB; Q6PDC8; -.
DR STRING; 10090.ENSMUSP00000107989; -.
DR iPTMnet; Q6PDC8; -.
DR PhosphoSitePlus; Q6PDC8; -.
DR SwissPalm; Q6PDC8; -.
DR PaxDb; Q6PDC8; -.
DR PRIDE; Q6PDC8; -.
DR ProteomicsDB; 252543; -. [Q6PDC8-1]
DR ProteomicsDB; 252544; -. [Q6PDC8-2]
DR ProteomicsDB; 252545; -. [Q6PDC8-3]
DR ProteomicsDB; 252546; -. [Q6PDC8-4]
DR Antibodypedia; 34569; 84 antibodies from 15 providers.
DR DNASU; 213006; -.
DR Ensembl; ENSMUST00000046658; ENSMUSP00000039635; ENSMUSG00000059149. [Q6PDC8-3]
DR Ensembl; ENSMUST00000112370; ENSMUSP00000107989; ENSMUSG00000059149. [Q6PDC8-4]
DR Ensembl; ENSMUST00000126927; ENSMUSP00000116706; ENSMUSG00000059149. [Q6PDC8-4]
DR Ensembl; ENSMUST00000144548; ENSMUSP00000116282; ENSMUSG00000059149. [Q6PDC8-4]
DR Ensembl; ENSMUST00000159038; ENSMUSP00000125558; ENSMUSG00000059149. [Q6PDC8-1]
DR GeneID; 213006; -.
DR KEGG; mmu:213006; -.
DR UCSC; uc007coe.2; mouse. [Q6PDC8-4]
DR UCSC; uc007cog.2; mouse. [Q6PDC8-3]
DR UCSC; uc011wrs.1; mouse. [Q6PDC8-2]
DR UCSC; uc011wru.1; mouse. [Q6PDC8-1]
DR CTD; 148808; -.
DR MGI; MGI:2442786; Mfsd4a.
DR VEuPathDB; HostDB:ENSMUSG00000059149; -.
DR eggNOG; ENOG502QRVK; Eukaryota.
DR GeneTree; ENSGT00530000063320; -.
DR InParanoid; Q6PDC8; -.
DR OMA; EAWRWSN; -.
DR OrthoDB; 952222at2759; -.
DR PhylomeDB; Q6PDC8; -.
DR TreeFam; TF314613; -.
DR BioGRID-ORCS; 213006; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Mfsd4a; mouse.
DR PRO; PR:Q6PDC8; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q6PDC8; protein.
DR Bgee; ENSMUSG00000059149; Expressed in epithelium of stomach and 206 other tissues.
DR ExpressionAtlas; Q6PDC8; baseline and differential.
DR Genevisible; Q6PDC8; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005355; F:glucose transmembrane transporter activity; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..510
FT /note="Major facilitator superfamily domain-containing
FT protein 4A"
FT /id="PRO_0000273396"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..222
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_022541"
FT VAR_SEQ 228..379
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_022542"
FT VAR_SEQ 493..510
FT /note="VLTQDKALGVENPECYQR -> GENMILIAASSTERETHHHP (in
FT isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_022543"
SQ SEQUENCE 510 AA; 56183 MW; 0275FCC8C69BBD4C CRC64;
MGCDGRVSEL LRRNLQPTLT YWSVFFSFGL CIAFLGPTLL DLRCQTHSSL PQISWVFFSQ
QLCLLLGSAL GGVFKRTLAQ SLWALFTSTL VISLVFAVIP FCHDVKVLAS VIALAGLAMG
CIDTVANMQL VRIYQKDSAF FLQVLHFFVG LGALLSPLIA DPFLSEANCF PANNTANATS
RSHGSRVLSQ HHAAAQPWIN QTIPRLPPKE VTENHVSYAF WIMALINLPV PLAVLFLLSK
ERLLTCAQRK PLLLSADELA LETRPAEKED TSSLAPKFQP HSGQEDLFSC CQRKNFRGAP
CSFFAIHITA ALVLFMTDGM MGAYSAFVYS YAVEKPLSIG HKSAGYLPSL FWGFITLGRF
ISIPVSSRMR PATMVFINVV GVVVTFLMLL IFSYNVIFLF VGTASLGLFL SSTFPSMLAY
TEDILQYKGC ATTVLVTGAG IGEMVLQMLV GLIFQAQGSY SFLVCGVIFG CLAFIFYILL
LFFHRIHPEL SSVLTQDKAL GVENPECYQR
