MFD_HAEIN
ID MFD_HAEIN Reviewed; 1146 AA.
AC P45128;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000255|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000255|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000255|HAMAP-Rule:MF_00969}; OrderedLocusNames=HI_1258;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000255|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000255|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000255|HAMAP-Rule:MF_00969}.
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DR EMBL; L42023; AAC22905.1; -; Genomic_DNA.
DR PIR; I64112; I64112.
DR RefSeq; NP_439413.1; NC_000907.1.
DR RefSeq; WP_005694314.1; NC_000907.1.
DR AlphaFoldDB; P45128; -.
DR SMR; P45128; -.
DR STRING; 71421.HI_1258; -.
DR EnsemblBacteria; AAC22905; AAC22905; HI_1258.
DR KEGG; hin:HI_1258; -.
DR PATRIC; fig|71421.8.peg.1310; -.
DR eggNOG; COG1197; Bacteria.
DR HOGENOM; CLU_005122_0_3_6; -.
DR OMA; WAPPCRE; -.
DR PhylomeDB; P45128; -.
DR BioCyc; HINF71421:G1GJ1-1286-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0015616; F:DNA translocase activity; IBA:GO_Central.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0043175; F:RNA polymerase core enzyme binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR Gene3D; 3.90.1150.50; -; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR036101; CarD-like/TRCF_dom_sf.
DR InterPro; IPR003711; CarD-like/TRCF_domain.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR Pfam; PF02559; CarD_CdnL_TRCF; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; SSF141259; 1.
DR SUPFAM; SSF143517; SSF143517; 1.
DR SUPFAM; SSF52540; SSF52540; 4.
DR TIGRFAMs; TIGR00580; mfd; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA-binding; Helicase;
KW Hydrolase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1146
FT /note="Transcription-repair-coupling factor"
FT /id="PRO_0000102167"
FT DOMAIN 617..778
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
FT DOMAIN 800..953
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
FT MOTIF 731..734
FT /note="DEEH box"
FT BINDING 630..637
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
SQ SEQUENCE 1146 AA; 130130 MW; 6E3052B431978475 CRC64;
MKTAFFQPDI PTQPNDHKIL GNVLPGADAL AISEISEQNQ NLTVVVTPDT RSAVRLSRVL
SELSSQDVCL FPDWETLPYD TFSPHQEIIS SRLSALFHLQ NAKKGIFLLP ISTLMQRLCP
PQYLQHNVLL IKKGDRLVID KMRLQLEAAG YRAVEQVLEH GEYAVRGALL DLFPMGSAVP
FRLDFFDDEI DSIRTFDVDT QRTLDEISSI NLLPAHEFPT DDKGIEFFRA QFRETFGEIR
RDPEHIYQQI SKGTLISGIE YWQPLFFAEM ATLFDYLPEQ TLFVDMENNQ TQGERFYQDA
KQRYEQRKVD PMRPLLSPEK LWLNVDEVNR RLKSYPRITF KAEKVRSSVR QKNLPVAALP
EVTIQSQQKE PLGQLRQFIE HFKGNVLFSV ETEGRRETLL DLLSPLKLKP KQIQSLEQIE
NEKFSLLVSS LEQGFIIEQS LPVAIIGEAN LLGKRIQQRS RDKRKTINPD TLVRNLAELK
IGQPVVHLDH GVGRYGGLVT LDTGGIKAEY LLLNYANESK LYVPVTSLHL ISRYVGGSDE
SAPLHKLGNE AWAKSRQKAA EKIRDVAAEL LDVYAQREAK KGFAFKYDRE EFQQFSATFP
FEETYDQEMA INAVISDMCQ PKAMDRLVCG DVGFGKTEVA MRAAFLAVMN HKQVAVLVPT
TLLAQQHYEN FKDRFANLPV NVEVLSRFKT AKEQKQILEN LAEGKVDILI GTHKLIQSDV
KFNDLGLLII DEEHRFGVGQ KEKIKQLRAN IDILTLTATP IPRTLNMAMN GIRDLSIIST
PPARRLSIKT FVRQNDDLVV REAILREILR GGQVYYLHND VASIENTAEK LTALVPEARV
IVGHGQMRER ELERVMSDFY HQRYNVLVCS TIIETGIDVP TANTIIIERA DHFGLAQLHQ
LRGRVGRSHH QAYAYLLTPP PKMMTKDAER RLDALENLDN LGAGFILATH DLEIRGAGEL
LGNEQSGQIE SIGFSLYMEL LDAAVKALKE GREPSLEELT QQQADIELRV PALLPDDYLG
DVNMRLSFYK RIAAAESKAE LDELKVELID RFGLLPDATK NLLQITELRL LVEPLNVVRI
DAGTQGGFIE FSAKAQVNPD KFIQLIQKEP IVYRFDGPFK FKFMKDLSDN KVRLEFVVDL
LRTIAA
