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MFD_MYCTU
ID   MFD_MYCTU               Reviewed;        1234 AA.
AC   P9WMQ5; L0T5H3; P64326; P96380;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Transcription-repair-coupling factor {ECO:0000255|HAMAP-Rule:MF_00969};
DE            Short=TRCF {ECO:0000255|HAMAP-Rule:MF_00969};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00969};
GN   Name=mfd {ECO:0000255|HAMAP-Rule:MF_00969}; OrderedLocusNames=Rv1020;
GN   ORFNames=MTCY10G2.29c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   INTERACTION WITH RPOB.
RC   STRAIN=Erdman;
RX   PubMed=22904282; DOI=10.1128/jb.00879-12;
RA   Weiss L.A., Harrison P.G., Nickels B.E., Glickman M.S., Campbell E.A.,
RA   Darst S.A., Stallings C.L.;
RT   "Interaction of CarD with RNA polymerase mediates Mycobacterium
RT   tuberculosis viability, rifampicin resistance, and pathogenesis.";
RL   J. Bacteriol. 194:5621-5631(2012).
CC   -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC       polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC       release of RNAP and its truncated transcript from the DNA, and
CC       recruitment of nucleotide excision repair machinery to the damaged
CC       site. {ECO:0000255|HAMAP-Rule:MF_00969}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase subunit beta (RpoB).
CC       {ECO:0000269|PubMed:22904282}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00969}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC       {ECO:0000255|HAMAP-Rule:MF_00969}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC       RecG subfamily. {ECO:0000255|HAMAP-Rule:MF_00969}.
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DR   EMBL; AL123456; CCP43770.1; -; Genomic_DNA.
DR   PIR; G70622; G70622.
DR   RefSeq; NP_215536.1; NC_000962.3.
DR   RefSeq; WP_003405273.1; NZ_NVQJ01000018.1.
DR   PDB; 6ACA; X-ray; 3.60 A; A=1-1234.
DR   PDBsum; 6ACA; -.
DR   AlphaFoldDB; P9WMQ5; -.
DR   SMR; P9WMQ5; -.
DR   IntAct; P9WMQ5; 1.
DR   STRING; 83332.Rv1020; -.
DR   PaxDb; P9WMQ5; -.
DR   DNASU; 886077; -.
DR   GeneID; 886077; -.
DR   KEGG; mtu:Rv1020; -.
DR   TubercuList; Rv1020; -.
DR   eggNOG; COG1197; Bacteria.
DR   OMA; WAPPCRE; -.
DR   PhylomeDB; P9WMQ5; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0015616; F:DNA translocase activity; IBA:GO_Central.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0043175; F:RNA polymerase core enzyme binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 2.
DR   Gene3D; 3.90.1150.50; -; 1.
DR   HAMAP; MF_00969; TRCF; 1.
DR   InterPro; IPR036101; CarD-like/TRCF_dom_sf.
DR   InterPro; IPR003711; CarD-like/TRCF_domain.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR004576; Mfd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR037235; TRCF-like_C_D7.
DR   InterPro; IPR005118; TRCF_C.
DR   InterPro; IPR041471; UvrB_inter.
DR   Pfam; PF02559; CarD_CdnL_TRCF; 1.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF03461; TRCF; 1.
DR   Pfam; PF17757; UvrB_inter; 1.
DR   SMART; SM01058; CarD_TRCF; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00982; TRCF; 1.
DR   SUPFAM; SSF141259; SSF141259; 1.
DR   SUPFAM; SSF143517; SSF143517; 1.
DR   SUPFAM; SSF52540; SSF52540; 4.
DR   TIGRFAMs; TIGR00580; mfd; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..1234
FT                   /note="Transcription-repair-coupling factor"
FT                   /id="PRO_0000102170"
FT   DOMAIN          663..824
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
FT   DOMAIN          842..999
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
FT   REGION          1207..1234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           777..780
FT                   /note="DEEQ box"
FT   BINDING         676..683
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
SQ   SEQUENCE   1234 AA;  132908 MW;  DBE48CE5ACF42B7D CRC64;
     MTAPGPACSD TPIAGLVELA LSAPTFQQLM QRAGGRPDEL TLIAPASARL LVASALARQG
     PLLVVTATGR EADDLAAELR GVFGDAVALL PSWETLPHER LSPGVDTVGT RLMALRRLAH
     PDDAQLGPPL GVVVTSVRSL LQPMTPQLGM MEPLTLTVGD ESPFDGVVAR LVELAYTRVD
     MVGRRGEFAV RGGILDIFAP TAEHPVRVEF WGDEITEMRM FSVADQRSIP EIDIHTLVAF
     ACRELLLSED VRARAAQLAA RHPAAESTVT GSASDMLAKL AEGIAVDGME AVLPVLWSDG
     HALLTDQLPD GTPVLVCDPE KVRTRAADLI RTGREFLEAS WSVAALGTAE NQAPVDVEQL
     GGSGFVELDQ VRAAAARTGH PWWTLSQLSD ESAIELDVRA APSARGHQRD IDEIFAMLRA
     HIATGGYAAL VAPGTGTAHR VVERLSESDT PAGMLDPGQA PKPGVVGVLQ GPLRDGVIIP
     GANLVVITET DLTGSRVSAA EGKRLAAKRR NIVDPLALTA GDLVVHDQHG IGRFVEMVER
     TVGGARREYL VLEYASAKRG GGAKNTDKLY VPMDSLDQLS RYVGGQAPAL SRLGGSDWAN
     TKTKARRAVR EIAGELVSLY AKRQASPGHA FSPDTPWQAE LEDAFGFTET VDQLTAIEEV
     KADMEKPIPM DRVICGDVGY GKTEIAVRAA FKAVQDGKQV AVLVPTTLLA DQHLQTFGER
     MSGFPVTIKG LSRFTDAAES RAVIDGLADG SVDIVIGTHR LLQTGVRWKD LGLVVVDEEQ
     RFGVEHKEHI KSLRTHVDVL TMSATPIPRT LEMSLAGIRE MSTILTPPEE RYPVLTYVGP
     HDDKQIAAAL RRELLRDGQA FYVHNRVSSI DAAAARVREL VPEARVVVAH GQMPEDLLET
     TVQRFWNREH DILVCTTIVE TGLDISNANT LIVERADTFG LSQLHQLRGR VGRSRERGYA
     YFLYPPQVPL TETAYDRLAT IAQNNELGAG MAVALKDLEI RGAGNVLGIE QSGHVAGVGF
     DLYVRLVGEA LETYRDAYRA AADGQTVRTA EEPKDVRIDL PVDAHLPPDY IASDRLRLEG
     YRRLAAASSD REVAAVVDEL TDRYGALPEP ARRLAAVARL RLLCRGSGIT DVTAASAATV
     RLSPLTLPDS AQVRLKRMYP GAHYRATTAT VQVPIPRAGG LGAPRIRDVE LVQMVADLIT
     ALAGKPRQHI GITNPSPPGE DGRGRNTTIK ERQP
 
 
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