MFD_RICFE
ID MFD_RICFE Reviewed; 1142 AA.
AC Q4UMJ0;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000255|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000255|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000255|HAMAP-Rule:MF_00969}; OrderedLocusNames=RF_0367;
OS Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX NCBI_TaxID=315456;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC VR-1525 / URRWXCal2;
RX PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA Parinello H., Claverie J.-M., Raoult D.;
RT "The genome sequence of Rickettsia felis identifies the first putative
RT conjugative plasmid in an obligate intracellular parasite.";
RL PLoS Biol. 3:1-12(2005).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000255|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000255|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000255|HAMAP-Rule:MF_00969}.
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DR EMBL; CP000053; AAY61218.1; -; Genomic_DNA.
DR RefSeq; WP_011270706.1; NC_007109.1.
DR AlphaFoldDB; Q4UMJ0; -.
DR SMR; Q4UMJ0; -.
DR STRING; 315456.RF_0367; -.
DR EnsemblBacteria; AAY61218; AAY61218; RF_0367.
DR KEGG; rfe:RF_0367; -.
DR eggNOG; COG1197; Bacteria.
DR HOGENOM; CLU_005122_3_2_5; -.
DR OMA; WAPPCRE; -.
DR OrthoDB; 234717at2; -.
DR Proteomes; UP000008548; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 2.
DR Gene3D; 3.90.1150.50; -; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR036101; CarD-like/TRCF_dom_sf.
DR InterPro; IPR003711; CarD-like/TRCF_domain.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR Pfam; PF02559; CarD_CdnL_TRCF; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; SSF141259; 1.
DR SUPFAM; SSF143517; SSF143517; 1.
DR SUPFAM; SSF52540; SSF52540; 4.
DR TIGRFAMs; TIGR00580; mfd; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA-binding; Helicase;
KW Hydrolase; Nucleotide-binding.
FT CHAIN 1..1142
FT /note="Transcription-repair-coupling factor"
FT /id="PRO_0000281073"
FT DOMAIN 592..757
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
FT DOMAIN 778..932
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
FT MOTIF 710..713
FT /note="DEEQ box"
FT BINDING 605..612
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00969"
SQ SEQUENCE 1142 AA; 129663 MW; CC689B86E2253EC0 CRC64;
MLQQKFPATA KSFFATHNFT KNLKQDFILS SSNEDEALQL YKQALFFSSN ENIYYFPSYD
TIPYDHTSPN ANILSRRAEI LTKLTTNNSN GKLLITYAAN LLNKLPPKDF FSKYFLKLSP
KMKFTTDELA MFLVENSFTR NASSIDVGEF AVRGEIIDII LPGPKAYRIN FSWDYIESIK
EFDIDTQIST KSCSELVISP ANEIVLNSET NGNFKNNYLR NFGVNHTDNP LYEAVISGRK
FAGYEQLLPL FYNSCSSLVD YLNDPIFIFD NLSKQAILEF EHSYNDFYSA RSEVNKLKFN
SFYPTLSPTD LYFTASEITE LLEQKNNIFI SYENSEQATL IENISSASFI EKKTVFDKLF
EIIKAHSSKK IVIGAASLSG LERIKSITQN YEYKYNEINK LDEAKASVIN VGIIPLNQSF
YTKEYLFITA SELLEEKSSS TNTNKKLKNI LLELDNLAEG EFVVHKDHGI GQFLKLEALE
IKGKLHDFLK ILYAGNDKLY IPVENIEVIK KYGNDNAELD KLGSSSWQRS KAKLKNRIKE
IALHLIQIAA KRKLNSSASV EFDLEEYDKF CANFPFSETE DQLTAINDIK EDLRNGMLMD
RLICGDVGFG KTEVAMRAVF MVAKSLNEHL PQVAVVVPTT ILCSQHFSRF IERFKGFGLN
IKQLSSVISA KEAKIIRSEL ESGKINIIIG THSLLHKNTK FFNLKLLIID EEQHFGVGQK
EFLKSLKSSS HVLAMSATPI PRTLQMSMTG LKELSIIATP PLNRLEVRTM VMPFDPVIIR
DALLREHFRG GRSFYVVPRI KDIEDIEKQL KQIVPELSYK IAHGKMTPSK IDEVMSEFYA
GKFDILVSTT IIESGIDIAE ANTMVIHKAD MLGLSQLYQL RGRIGRGKVR GYAYLTVASH
KKMTSHSLRR LEIIQNSCAL GSGFTIASHD MDLRGFGNLI GEEQSGQIKE VGTELYQEML
EEQIAIFKDE PIVSEQLFIP TINLGLSVFI PDNYISDSAL KLGLYRRIGN LSNEIEVEKF
KDEMIDRFGS LPIEFNNLLD IVKIKLLCFK LNIENLDSGD NGFVIKFYKN ADMTDKILKF
VSTYSNQAKI KPDNKLVYIK KLVDKNIIVE VNQLLWKLGG CRLDVIPNTS LRGAKRRGNL
IK
