MFEB_DICDI
ID MFEB_DICDI Reviewed; 294 AA.
AC Q54XZ0; Q86SA5;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Probable enoyl-CoA hydratase 2;
DE EC=4.2.1.119;
DE AltName: Full=MFE-2;
DE AltName: Full=Multifunctional enzyme B;
DE Short=MFE-B;
GN Name=mfeB; Synonyms=mfe2; ORFNames=DDB_G0277911;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 80-294.
RC STRAIN=AX4;
RX PubMed=12796309; DOI=10.1128/ec.2.3.638-645.2003;
RA Matsuoka S., Saito T., Kuwayama H., Morita N., Ochiai H., Maeda M.;
RT "MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase
RT family, affects fatty acid metabolism necessary for morphogenesis in
RT Dictyostelium spp.";
RL Eukaryot. Cell 2:638-645(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:26526, ChEBI:CHEBI:15377, ChEBI:CHEBI:57319,
CC ChEBI:CHEBI:58856; EC=4.2.1.119;
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAFI02000023; EAL68128.1; -; Genomic_DNA.
DR EMBL; AB100096; BAC55274.1; -; mRNA.
DR RefSeq; XP_642412.1; XM_637320.1.
DR AlphaFoldDB; Q54XZ0; -.
DR SMR; Q54XZ0; -.
DR STRING; 44689.DDB0214811; -.
DR PaxDb; Q54XZ0; -.
DR EnsemblProtists; EAL68128; EAL68128; DDB_G0277911.
DR GeneID; 8621617; -.
DR KEGG; ddi:DDB_G0277911; -.
DR dictyBase; DDB_G0277911; mfeB.
DR eggNOG; KOG1206; Eukaryota.
DR HOGENOM; CLU_040078_1_0_1; -.
DR InParanoid; Q54XZ0; -.
DR OMA; FKHTDQE; -.
DR PhylomeDB; Q54XZ0; -.
DR Reactome; R-DDI-193368; Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
DR Reactome; R-DDI-2046106; alpha-linolenic acid (ALA) metabolism.
DR Reactome; R-DDI-389887; Beta-oxidation of pristanoyl-CoA.
DR Reactome; R-DDI-390247; Beta-oxidation of very long chain fatty acids.
DR Reactome; R-DDI-9033241; Peroxisomal protein import.
DR PRO; PR:Q54XZ0; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005777; C:peroxisome; IBA:GO_Central.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0044594; F:17-beta-hydroxysteroid dehydrogenase (NAD+) activity; IBA:GO_Central.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IBA:GO_Central.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IBA:GO_Central.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR002539; MaoC-like_dom.
DR Pfam; PF01575; MaoC_dehydratas; 1.
DR SUPFAM; SSF54637; SSF54637; 2.
PE 2: Evidence at transcript level;
KW Lyase; Peroxisome; Reference proteome.
FT CHAIN 1..294
FT /note="Probable enoyl-CoA hydratase 2"
FT /id="PRO_0000328621"
FT DOMAIN 165..269
FT /note="MaoC-like"
FT MOTIF 292..294
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
FT BINDING 84..85
FT /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT /ligand_id="ChEBI:CHEBI:74272"
FT /evidence="ECO:0000250"
FT BINDING 113
FT /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT /ligand_id="ChEBI:CHEBI:74272"
FT /evidence="ECO:0000250"
FT BINDING 190..195
FT /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT /ligand_id="ChEBI:CHEBI:74272"
FT /evidence="ECO:0000250"
FT BINDING 213
FT /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT /ligand_id="ChEBI:CHEBI:74272"
FT /evidence="ECO:0000250"
FT BINDING 243
FT /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT /ligand_id="ChEBI:CHEBI:74272"
FT /evidence="ECO:0000250"
SQ SEQUENCE 294 AA; 32396 MW; BDE5137A023D3C6E CRC64;
MTVDVKKVIN HKIKPIEYNL TRKDVALYAI SLGCGKKHLK FVYEGSDNFS ALPTLGVIFP
GQMIVDVISE GIDGIEFDPM MLLHGEQELE ILNEIPVEGV FVTESKITNL YDKGKGALLI
LQCITSEKSS GKPIFKNIFS FFIRGIGGFG GDRGPNEKPI QIPKDRAPDA ISKQATSEDQ
AVIYRLAGGD LNPLHIDPEM SKIGGFEVPI LHGLCTYGIA SRGVLEHFCD NDPSRLKSIK
TRFTKHVYPG ETIETEMWKI NPTTILFQSK TNRDGSYVLS SGVAIIEPIK KGSL
