MFI_MOUSE
ID MFI_MOUSE Reviewed; 315 AA.
AC Q9D4W2; Q3U5F8;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Protein MFI {ECO:0000305};
DE AltName: Full=Mitochondrial fission factor interactor {ECO:0000303|PubMed:30059978};
DE AltName: Full=Protein C11orf65 homolog;
GN Name=Mfi {ECO:0000303|PubMed:30059978};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH MFF, SUBCELLULAR LOCATION,
RP AND SUBUNIT.
RX PubMed=30059978; DOI=10.1210/en.2018-00426;
RA Lee J., Pappalardo Z., Chopra D.G., Hennings T.G., Vaughn I., Lan C.,
RA Choe J.J., Ang K., Chen S., Arkin M., McManus M.T., German M.S., Ku G.M.;
RT "A Genetic Interaction Map of Insulin Production Identifies Mfi as an
RT Inhibitor of Mitochondrial Fission.";
RL Endocrinology 159:3321-3330(2018).
CC -!- FUNCTION: Acts as an inhibitor of mitochondrial fission. Interacts with
CC MFF and prevents DNM1L recruitment to mitochondria, promoting a more
CC fused mitochondrial network. {ECO:0000269|PubMed:30059978}.
CC -!- SUBUNIT: Can homodimerize (PubMed:30059978). Interacts with MFF; the
CC interaction inhibits MFF interaction with DNM1L (PubMed:30059978).
CC {ECO:0000269|PubMed:30059978}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:30059978}.
CC Mitochondrion outer membrane {ECO:0000269|PubMed:30059978}.
CC Note=Predominantly localizes to the cytosol, with a minor fraction at
CC the outer mitochondrial membrane. {ECO:0000269|PubMed:30059978}.
CC -!- TISSUE SPECIFICITY: Enriched in the pancreatic beta cell and the testis
CC and is expressed at low levels in other tissues tested.
CC {ECO:0000269|PubMed:30059978}.
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DR EMBL; AK016083; BAB30107.1; -; mRNA.
DR EMBL; AK153618; BAE32121.1; -; mRNA.
DR EMBL; BC115639; AAI15640.1; -; mRNA.
DR CCDS; CCDS23183.1; -.
DR RefSeq; NP_083523.1; NM_029247.3.
DR RefSeq; XP_006510715.1; XM_006510652.3.
DR RefSeq; XP_006510716.1; XM_006510653.3.
DR RefSeq; XP_006510717.1; XM_006510654.3.
DR RefSeq; XP_017169167.1; XM_017313678.1.
DR AlphaFoldDB; Q9D4W2; -.
DR SMR; Q9D4W2; -.
DR STRING; 10090.ENSMUSP00000005262; -.
DR iPTMnet; Q9D4W2; -.
DR PhosphoSitePlus; Q9D4W2; -.
DR PaxDb; Q9D4W2; -.
DR PRIDE; Q9D4W2; -.
DR Antibodypedia; 50806; 71 antibodies from 13 providers.
DR Ensembl; ENSMUST00000005262; ENSMUSP00000005262; ENSMUSG00000005131.
DR GeneID; 75311; -.
DR KEGG; mmu:75311; -.
DR UCSC; uc009pmc.1; mouse.
DR MGI; MGI:1922561; 4930550C14Rik.
DR VEuPathDB; HostDB:ENSMUSG00000005131; -.
DR eggNOG; ENOG502QSTG; Eukaryota.
DR GeneTree; ENSGT00510000048394; -.
DR HOGENOM; CLU_068479_0_0_1; -.
DR InParanoid; Q9D4W2; -.
DR OMA; EEYMEEW; -.
DR OrthoDB; 1265232at2759; -.
DR PhylomeDB; Q9D4W2; -.
DR TreeFam; TF328500; -.
DR BioGRID-ORCS; 75311; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q9D4W2; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9D4W2; protein.
DR Bgee; ENSMUSG00000005131; Expressed in animal zygote and 59 other tissues.
DR ExpressionAtlas; Q9D4W2; baseline and differential.
DR Genevisible; Q9D4W2; MM.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:UniProtKB.
DR GO; GO:0090258; P:negative regulation of mitochondrial fission; IMP:UniProtKB.
DR GO; GO:1903215; P:negative regulation of protein targeting to mitochondrion; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Cytoplasm; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW Reference proteome.
FT CHAIN 1..315
FT /note="Protein MFI"
FT /id="PRO_0000263666"
FT CONFLICT 233
FT /note="V -> E (in Ref. 1; BAE32121)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 315 AA; 36933 MW; 200CE498C007D3F1 CRC64;
MSVKHIQDNS NQEIAARIIQ RAWKTFLNVS VYQHFKSLID LRRQGEPRQI VRYINPKEAQ
LLDAAAGVQV RFRLGGVRFP PEIYYKIFTH RHIEDLCANS PRDYTKLPAR YTSHNKDDPP
QVEDNSGWYR RVENNGWRPV SYRFWMPLES GVVDSTKESE FHFSKLKRKQ DLEKKRKIKK
IDWMRQMYYM GSLEAKATDN ETLGLIHKAT KGLIKSIEDG GVDSVMEWEV DEVLNWTNTL
NFDEYIASWR ETATSNSSAN LKDVKLQRIQ KSLQSNIYGD EAKQAEESLY DDSTYYENAY
TKQFTRLTPD SMFGM
