ID   MFM2_SCHPO              Reviewed;          44 AA.
AC   P34069;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 125.
DE   RecName: Full=M-factor;
DE   Flags: Precursor;
GN   Name=mfm2; ORFNames=SPAC513.03;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 33-41,
RP   ISOPRENYLATION AT CYS-41, AND METHYLATION AT CYS-41.
RX   PubMed=1547790; DOI=10.1002/j.1460-2075.1992.tb05134.x;
RA   Davey J.;
RT   "Mating pheromones of the fission yeast Schizosaccharomyces pombe:
RT   purification and structural characterization of M-factor and isolation and
RT   analysis of two genes encoding the pheromone.";
RL   EMBO J. 11:951-960(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: M-factor is a mating pheromone produced by M-type mating
CC       cells. All three mfm genes contribute to the production of M-factor.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC   -!- INDUCTION: By nitrogen starvation. It is further induced by a pheromone
CC       signal. Its transcription is limited to M cells.
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DR   EMBL; X63628; CAA45176.1; -; Genomic_DNA.
DR   EMBL; CU329670; CAB58726.1; -; Genomic_DNA.
DR   PIR; S21028; S21028.
DR   RefSeq; NP_593977.1; NM_001019403.2.
DR   AlphaFoldDB; P34069; -.
DR   BioGRID; 279914; 25.
DR   STRING; 4896.SPAC513.03.1; -.
DR   PaxDb; P34069; -.
DR   EnsemblFungi; SPAC513.03.1; SPAC513.03.1:pep; SPAC513.03.
DR   GeneID; 2543495; -.
DR   KEGG; spo:SPAC513.03; -.
DR   PomBase; SPAC513.03; mfm2.
DR   VEuPathDB; FungiDB:SPAC513.03; -.
DR   HOGENOM; CLU_3260799_0_0_1; -.
DR   PhylomeDB; P34069; -.
DR   PRO; PR:P34069; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005576; C:extracellular region; ISS:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000772; F:mating pheromone activity; IMP:PomBase.
DR   GO; GO:0007267; P:cell-cell signaling; IMP:PomBase.
DR   GO; GO:0062038; P:positive regulation of pheromone response MAPK cascade; IDA:PomBase.
DR   GO; GO:0032005; P:signal transduction involved in positive regulation of conjugation with cellular fusion; NAS:PomBase.
DR   InterPro; IPR005555; M-factor.
DR   Pfam; PF03855; M-factor; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Lipoprotein; Membrane;
KW   Methylation; Pheromone; Prenylation; Reference proteome; Stress response.
FT   PROPEP          1..32
FT                   /evidence="ECO:0000269|PubMed:1547790"
FT                   /id="PRO_0000021710"
FT   PEPTIDE         33..41
FT                   /note="M-factor"
FT                   /id="PRO_0000021711"
FT   PROPEP          42..44
FT                   /note="Removed in mature form"
FT                   /id="PRO_0000021712"
FT   MOD_RES         41
FT                   /note="Cysteine methyl ester"
FT                   /evidence="ECO:0000269|PubMed:1547790"
FT   LIPID           41
FT                   /note="S-farnesyl cysteine"
FT                   /evidence="ECO:0000269|PubMed:1547790"
SQ   SEQUENCE   44 AA;  4858 MW;  7007642A876BF77E CRC64;
     MDSIATNTHS SSIVNAYNNN PTDVVKTQNI KNYTPKVPYM CVIA