ID   MFNB_METAC              Reviewed;         234 AA.
AC   Q8THS6;
DT   19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=(5-formylfuran-3-yl)methyl phosphate synthase {ECO:0000255|HAMAP-Rule:MF_00681};
DE            EC=4.2.3.153 {ECO:0000255|HAMAP-Rule:MF_00681};
DE   AltName: Full=4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase {ECO:0000255|HAMAP-Rule:MF_00681};
DE            Short=4-HFC-P synthase {ECO:0000255|HAMAP-Rule:MF_00681};
GN   Name=mfnB {ECO:0000255|HAMAP-Rule:MF_00681}; OrderedLocusNames=MA_4436;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the formation of 4-(hydroxymethyl)-2-
CC       furancarboxaldehyde phosphate (4-HFC-P) from two molecules of
CC       glyceraldehyde-3-P (GA-3-P). {ECO:0000255|HAMAP-Rule:MF_00681}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-
CC         furancarboxaldehyde phosphate + 2 H2O + phosphate;
CC         Xref=Rhea:RHEA:43536, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:59776, ChEBI:CHEBI:83407; EC=4.2.3.153;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00681};
CC   -!- PATHWAY: Cofactor biosynthesis; methanofuran biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00681}.
CC   -!- SIMILARITY: Belongs to the MfnB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00681}.
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DR   EMBL; AE010299; AAM07777.1; -; Genomic_DNA.
DR   RefSeq; WP_011024314.1; NC_003552.1.
DR   AlphaFoldDB; Q8THS6; -.
DR   SMR; Q8THS6; -.
DR   STRING; 188937.MA_4436; -.
DR   EnsemblBacteria; AAM07777; AAM07777; MA_4436.
DR   GeneID; 1476330; -.
DR   KEGG; mac:MA_4436; -.
DR   HOGENOM; CLU_068659_0_0_2; -.
DR   InParanoid; Q8THS6; -.
DR   OMA; NFPWVIR; -.
DR   OrthoDB; 71837at2157; -.
DR   PhylomeDB; Q8THS6; -.
DR   UniPathway; UPA00080; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0016830; F:carbon-carbon lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:2001120; P:methanofuran biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00681; MfnB; 1.
DR   InterPro; IPR007565; 4HFCP_synth.
DR   InterPro; IPR035081; 4HFCP_synth_arc.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   Pfam; PF04476; 4HFCP_synth; 1.
DR   PIRSF; PIRSF015957; UCP015957; 1.
DR   SUPFAM; SSF51366; SSF51366; 1.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome; Schiff base.
FT   CHAIN           1..234
FT                   /note="(5-formylfuran-3-yl)methyl phosphate synthase"
FT                   /id="PRO_0000134864"
FT   ACT_SITE        27
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00681"
FT   ACT_SITE        85
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00681"
SQ   SEQUENCE   234 AA;  25098 MW;  FC275925AD1EB9CB CRC64;
     MKLLVSPINR EEAIIASLGG ADIVDVKNPK EGSLGANFPW VIRDVKEVVN GRQPISATIG
     DFNYKPGTAS LAALGAAVAG ADYIKVGLYD IQTEAQALEL LTKITLAVKD YDPSKKVVAS
     GYSDYKRINS ISPLLLPAVA AEAGVDVVMV DTGIKDGKST FEFMDEQELK EFTDLAHEHG
     LENAIAGSLK FEDLPVLERI GPDIIGVRGM VCGGDRRTAI RQELVEKLVA ECQI