MFNB_METVS
ID MFNB_METVS Reviewed; 236 AA.
AC A6USK6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=(5-formylfuran-3-yl)methyl phosphate synthase {ECO:0000255|HAMAP-Rule:MF_00681};
DE EC=4.2.3.153 {ECO:0000255|HAMAP-Rule:MF_00681};
DE AltName: Full=4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase {ECO:0000255|HAMAP-Rule:MF_00681};
DE Short=4-HFC-P synthase {ECO:0000255|HAMAP-Rule:MF_00681};
GN Name=mfnB {ECO:0000255|HAMAP-Rule:MF_00681}; OrderedLocusNames=Mevan_1586;
OS Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148
OS / SB).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanococcaceae; Methanococcus.
OX NCBI_TaxID=406327;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.,
RA Sieprawska-Lupa M., Whitman W.B., Richardson P.;
RT "Complete sequence of Methanococcus vannielii SB.";
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of 4-(hydroxymethyl)-2-
CC furancarboxaldehyde phosphate (4-HFC-P) from two molecules of
CC glyceraldehyde-3-P (GA-3-P). {ECO:0000255|HAMAP-Rule:MF_00681}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-
CC furancarboxaldehyde phosphate + 2 H2O + phosphate;
CC Xref=Rhea:RHEA:43536, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:59776, ChEBI:CHEBI:83407; EC=4.2.3.153;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00681};
CC -!- PATHWAY: Cofactor biosynthesis; methanofuran biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00681}.
CC -!- SIMILARITY: Belongs to the MfnB family. {ECO:0000255|HAMAP-
CC Rule:MF_00681}.
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DR EMBL; CP000742; ABR55478.1; -; Genomic_DNA.
DR RefSeq; WP_012066392.1; NC_009634.1.
DR AlphaFoldDB; A6USK6; -.
DR SMR; A6USK6; -.
DR STRING; 406327.Mevan_1586; -.
DR EnsemblBacteria; ABR55478; ABR55478; Mevan_1586.
DR GeneID; 5324758; -.
DR KEGG; mvn:Mevan_1586; -.
DR eggNOG; arCOG04482; Archaea.
DR HOGENOM; CLU_068659_0_0_2; -.
DR OMA; NFPWVIR; -.
DR OrthoDB; 71837at2157; -.
DR UniPathway; UPA00080; -.
DR Proteomes; UP000001107; Chromosome.
DR GO; GO:0016830; F:carbon-carbon lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:2001120; P:methanofuran biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00681; MfnB; 1.
DR InterPro; IPR007565; 4HFCP_synth.
DR InterPro; IPR035081; 4HFCP_synth_arc.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR Pfam; PF04476; 4HFCP_synth; 1.
DR PIRSF; PIRSF015957; UCP015957; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
PE 3: Inferred from homology;
KW Lyase; Schiff base.
FT CHAIN 1..236
FT /note="(5-formylfuran-3-yl)methyl phosphate synthase"
FT /id="PRO_1000044924"
FT ACT_SITE 27
FT /note="Schiff-base intermediate with substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00681"
FT ACT_SITE 85
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00681"
SQ SEQUENCE 236 AA; 25179 MW; A9C36C03883A0BAF CRC64;
MILLVSPKDV AEAYEAINGG ADIIDVKNPP EGSLGANFPW VIKEIRSATP NGMLVSAAIG
DVHYKPGTVT LAALGATISG ADYIKIGLYG TRSYQEAVDV MKNVSNAVKS EDPKKIVVAA
GYADAYRVGA VDPLIIPKIA RDSGCDVAML DTAVKDGKTL FDHLSIDLLK EFVEETHKYG
MKCALAGSIK KEEIPMLKEI GCDIVGIRGA ACTKGDRNEG KIQKDLVKEI VKICKE
