MFPA_MYCS2
ID MFPA_MYCS2 Reviewed; 191 AA.
AC A0QSY0;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Pentapeptide repeat protein MfpA {ECO:0000303|PubMed:11709313, ECO:0000305};
DE AltName: Full=Mycobacterial fluoroquinone resistance pentapeptide {ECO:0000303|PubMed:11709313};
DE AltName: Full=Orf3 {ECO:0000303|PubMed:11709313};
GN Name=mfpA {ECO:0000303|PubMed:11709313};
GN OrderedLocusNames=MSMEG_1641, MSMEI_1602;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND ANTIBIOTIC RESISTANCE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=11709313; DOI=10.1128/aac.45.12.3387-3392.2001;
RA Montero C., Mateu G., Rodriguez R., Takiff H.;
RT "Intrinsic resistance of Mycobacterium smegmatis to fluoroquinolones may be
RT influenced by new pentapeptide protein MfpA.";
RL Antimicrob. Agents Chemother. 45:3387-3392(2001).
CC -!- FUNCTION: When present on multicopy plasmids confers increased
CC resistance to fluoroquinolone antibiotics such as ciprofloxacin and
CC sparfloxacin but not the quinolone nalidixic acid (PubMed:11709313).
CC Forms a structure that exhibits size, shape and electrostatic
CC similarity to B-form DNA; it may bind to DNA gyrase which is postulated
CC to protect it from fluoroquinolones (By similarity).
CC {ECO:0000250|UniProtKB:I6YBX3, ECO:0000269|PubMed:11709313}.
CC -!- SUBUNIT: Homodimer. Probably interacts with DNA gyrase.
CC {ECO:0000250|UniProtKB:I6YBX3}.
CC -!- DOMAIN: Each subunit forms a right-handed beta-helix with 8 complete
CC coils that stack upon each other. {ECO:0000250|UniProtKB:I6YBX3}.
CC -!- DISRUPTION PHENOTYPE: Decreased resistance to fluoroquinolone
CC antibiotics ciprofloxacin and sparfloxacin.
CC {ECO:0000269|PubMed:11709313}.
CC -!- SIMILARITY: Belongs to the pentapeptide repeat protein family.
CC {ECO:0000305}.
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DR EMBL; CP000480; ABK73161.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP38075.1; -; Genomic_DNA.
DR RefSeq; WP_003893048.1; NZ_SIJM01000023.1.
DR RefSeq; YP_886018.1; NC_008596.1.
DR PDB; 6ZT4; X-ray; 1.77 A; A/B=1-191.
DR PDB; 6ZT5; X-ray; 2.20 A; A/B=1-191.
DR PDBsum; 6ZT4; -.
DR PDBsum; 6ZT5; -.
DR AlphaFoldDB; A0QSY0; -.
DR SMR; A0QSY0; -.
DR STRING; 246196.MSMEI_1602; -.
DR EnsemblBacteria; ABK73161; ABK73161; MSMEG_1641.
DR EnsemblBacteria; AFP38075; AFP38075; MSMEI_1602.
DR GeneID; 66733092; -.
DR KEGG; msg:MSMEI_1602; -.
DR KEGG; msm:MSMEG_1641; -.
DR PATRIC; fig|246196.19.peg.1626; -.
DR eggNOG; COG1357; Bacteria.
DR OMA; ADFFDCK; -.
DR OrthoDB; 1677662at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR InterPro; IPR001646; 5peptide_repeat.
DR Pfam; PF00805; Pentapeptide; 1.
DR Pfam; PF13599; Pentapeptide_4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic resistance; Reference proteome.
FT CHAIN 1..191
FT /note="Pentapeptide repeat protein MfpA"
FT /id="PRO_0000434154"
FT DOMAIN 115..154
FT /note="Pentapeptide repeat"
FT /evidence="ECO:0000255"
FT STRAND 11..14
FT /evidence="ECO:0007829|PDB:6ZT4"
FT STRAND 16..19
FT /evidence="ECO:0007829|PDB:6ZT4"
FT STRAND 31..34
FT /evidence="ECO:0007829|PDB:6ZT4"
FT STRAND 36..39
FT /evidence="ECO:0007829|PDB:6ZT4"
FT STRAND 51..54
FT /evidence="ECO:0007829|PDB:6ZT4"
FT STRAND 56..59
FT /evidence="ECO:0007829|PDB:6ZT4"
FT STRAND 71..74
FT /evidence="ECO:0007829|PDB:6ZT4"
FT STRAND 91..94
FT /evidence="ECO:0007829|PDB:6ZT4"
FT HELIX 161..164
FT /evidence="ECO:0007829|PDB:6ZT4"
FT HELIX 174..183
FT /evidence="ECO:0007829|PDB:6ZT4"
FT STRAND 187..189
FT /evidence="ECO:0007829|PDB:6ZT5"
SQ SEQUENCE 191 AA; 21232 MW; A6517548538C5173 CRC64;
MRIGANGDET VWADEEFAGR DFRDEDLSRI RTERVVFTEC DFSGVDLSES EHHGSAFRNC
TFRRSTIWHS TFTNCSLLGS VFTECRIRPV TFVECDFTLA VLGGCDLRAV DLSDCRLREV
SLVGADLRKA VLRRADLTGS RVQDARLEEA DLRGTRVDPT FWTTAKVRGA KIDIEQALAY
AAAHGLAVHG G
