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MFPS_AGRFC
ID   MFPS_AGRFC              Reviewed;         454 AA.
AC   A7TZT2; A9CK29;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Mannosylfructose-phosphate synthase {ECO:0000312|EMBL:ABU63292.1};
DE            EC=2.4.1.246;
GN   Name=mfpsA {ECO:0000312|EMBL:ABU63292.1}; OrderedLocusNames=Atu0661;
GN   ORFNames=AGR_C_1178;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ABU63292.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, COFACTOR,
RP   BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, AND INDUCTION.
RX   PubMed=17728402; DOI=10.1073/pnas.0706709104;
RA   Torres L.L., Salerno G.L.;
RT   "A metabolic pathway leading to mannosylfructose biosynthesis in
RT   Agrobacterium tumefaciens uncovers a family of mannosyltransferases.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:14318-14323(2007).
RN   [2] {ECO:0000312|EMBL:AAK86469.2}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [3] {ECO:0000312|EMBL:AAK86469.2}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-fructose 6-phosphate + GDP-alpha-D-mannose = beta-D-
CC         fructofuranosyl alpha-D-mannopyranoside 6(F)-phosphate + GDP + H(+);
CC         Xref=Rhea:RHEA:26039, ChEBI:CHEBI:15378, ChEBI:CHEBI:57527,
CC         ChEBI:CHEBI:57634, ChEBI:CHEBI:58189, ChEBI:CHEBI:58870;
CC         EC=2.4.1.246; Evidence={ECO:0000269|PubMed:17728402};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:17728402};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:17728402};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=1.5 mM for GDP-mannose {ECO:0000269|PubMed:17728402};
CC         KM=1.0 mM for fructose-6-phosphate {ECO:0000269|PubMed:17728402};
CC       pH dependence:
CC         Optimum pH is 8.0. {ECO:0000269|PubMed:17728402};
CC   -!- PATHWAY: Carbohydrate metabolism; mannosylfructose biosynthesis; beta-
CC       D-fructofuranosyl alpha-D-mannopyranoside from D-fructose 6-phosphate
CC       and GDP-alpha-D-mannose: step 1/2. {ECO:0000269|PubMed:17728402}.
CC   -!- INDUCTION: By salt. {ECO:0000269|PubMed:17728402}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. {ECO:0000255}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK86469.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; EF530045; ABU63292.1; -; Genomic_DNA.
DR   EMBL; AE007869; AAK86469.2; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_353684.2; NC_003062.2.
DR   AlphaFoldDB; A7TZT2; -.
DR   SMR; A7TZT2; -.
DR   STRING; 176299.Atu0661; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   EnsemblBacteria; AAK86469; AAK86469; Atu0661.
DR   KEGG; atu:Atu0661; -.
DR   PATRIC; fig|176299.10.peg.654; -.
DR   eggNOG; COG0438; Bacteria.
DR   HOGENOM; CLU_009583_2_3_5; -.
DR   BioCyc; AGRO:ATU0661-MON; -.
DR   BioCyc; MetaCyc:MON-14460; -.
DR   BRENDA; 2.4.1.246; 200.
DR   UniPathway; UPA01006; UER01009.
DR   Proteomes; UP000000813; Chromosome circular.
DR   GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0103011; F:mannosylfructose-phosphate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046351; P:disaccharide biosynthetic process; IDA:UniProtKB.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR028098; Glyco_trans_4-like_N.
DR   Pfam; PF13439; Glyco_transf_4; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..454
FT                   /note="Mannosylfructose-phosphate synthase"
FT                   /id="PRO_0000401102"
SQ   SEQUENCE   454 AA;  51911 MW;  D44776F9FEA75898 CRC64;
     MEKFTKMGPM TTTSETERYP RIALISTHGY VAAHPPLGAA DTGGQVVYVL ELARKLGQLG
     YTVDLYTRRF EDQPEFDEVD ERVRVVRIPC GGRDFIPKEY LHRHLMEWCE NALRFIKKND
     LNYSFINSHY WDAGVAGQRL SEALKIPHLH TPHSLGIWKK RQMETDYPEK ADTFELEFNF
     KERIQHELII YRSCDMVIAT TPVQLDVLIE DYGLKRKHIH MIPPGYDDNR FFPVSDATRQ
     MIRQRFGFEG KVVLALGRLA TNKGYDLLID GFSVLAEREP EARLHLAVGG ENMDEQETTI
     LNQLKERVKS LGLEDKVAFS GYVADEDLPD IYRAADLFVL SSRYEPFGMT AIEAMASGTP
     TVVTIHGGLF RAISYGRHAL FADPFDKEDL GITMMKPFKH ERLYGRLSRM GAHKARSLFT
     WTGIAQQLLA LVEGRTMMPV LEEADWAEPW NDGD
 
 
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