MFR1L_MOUSE
ID MFR1L_MOUSE Reviewed; 289 AA.
AC Q9CWE0; Q3UJK3; Q8BTP7;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Mitochondrial fission regulator 1-like;
GN Name=Mtfr1l; Synonyms=Fam54b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, DBA/2J, and NOD; TISSUE=Heart, Olfactory bulb, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=19131326; DOI=10.1074/mcp.m800451-mcp200;
RA Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
RT "Large scale localization of protein phosphorylation by use of electron
RT capture dissociation mass spectrometry.";
RL Mol. Cell. Proteomics 8:904-912(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38; SER-221 AND SER-222, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CWE0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CWE0-2; Sequence=VSP_034340;
CC -!- SIMILARITY: Belongs to the MTFR1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK010825; BAB27207.1; -; mRNA.
DR EMBL; AK089141; BAC40763.1; -; mRNA.
DR EMBL; AK145362; BAE26389.1; -; mRNA.
DR EMBL; AK146416; BAE27152.1; -; mRNA.
DR EMBL; AK164004; BAE37584.1; -; mRNA.
DR EMBL; AK169047; BAE40835.1; -; mRNA.
DR EMBL; AK171703; BAE42619.1; -; mRNA.
DR EMBL; BC026808; AAH26808.1; -; mRNA.
DR CCDS; CCDS18775.1; -. [Q9CWE0-1]
DR RefSeq; NP_001243041.1; NM_001256112.1. [Q9CWE0-1]
DR RefSeq; NP_084035.1; NM_029759.4. [Q9CWE0-1]
DR RefSeq; XP_006539319.1; XM_006539256.1. [Q9CWE0-1]
DR RefSeq; XP_006539320.1; XM_006539257.2.
DR RefSeq; XP_006539321.1; XM_006539258.2. [Q9CWE0-1]
DR AlphaFoldDB; Q9CWE0; -.
DR BioGRID; 218339; 5.
DR IntAct; Q9CWE0; 2.
DR MINT; Q9CWE0; -.
DR STRING; 10090.ENSMUSP00000099609; -.
DR iPTMnet; Q9CWE0; -.
DR PhosphoSitePlus; Q9CWE0; -.
DR SwissPalm; Q9CWE0; -.
DR EPD; Q9CWE0; -.
DR jPOST; Q9CWE0; -.
DR MaxQB; Q9CWE0; -.
DR PaxDb; Q9CWE0; -.
DR PeptideAtlas; Q9CWE0; -.
DR PRIDE; Q9CWE0; -.
DR ProteomicsDB; 293460; -. [Q9CWE0-1]
DR ProteomicsDB; 293461; -. [Q9CWE0-2]
DR Antibodypedia; 30499; 18 antibodies from 8 providers.
DR DNASU; 76824; -.
DR Ensembl; ENSMUST00000102550; ENSMUSP00000099609; ENSMUSG00000046671. [Q9CWE0-1]
DR Ensembl; ENSMUST00000116279; ENSMUSP00000111983; ENSMUSG00000046671. [Q9CWE0-1]
DR GeneID; 76824; -.
DR KEGG; mmu:76824; -.
DR UCSC; uc008vfi.2; mouse. [Q9CWE0-1]
DR CTD; 56181; -.
DR MGI; MGI:1924074; Mtfr1l.
DR VEuPathDB; HostDB:ENSMUSG00000046671; -.
DR eggNOG; ENOG502QRAC; Eukaryota.
DR GeneTree; ENSGT00950000183215; -.
DR InParanoid; Q9CWE0; -.
DR OMA; SATSECC; -.
DR OrthoDB; 1485791at2759; -.
DR PhylomeDB; Q9CWE0; -.
DR TreeFam; TF331404; -.
DR BioGRID-ORCS; 76824; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Mtfr1l; mouse.
DR PRO; PR:Q9CWE0; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9CWE0; protein.
DR Bgee; ENSMUSG00000046671; Expressed in embryonic brain and 250 other tissues.
DR ExpressionAtlas; Q9CWE0; baseline and differential.
DR Genevisible; Q9CWE0; MM.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0000266; P:mitochondrial fission; IBA:GO_Central.
DR InterPro; IPR007972; Mtfr1.
DR PANTHER; PTHR14215; PTHR14215; 1.
DR Pfam; PF05308; Mito_fiss_reg; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..289
FT /note="Mitochondrial fission regulator 1-like"
FT /id="PRO_0000341567"
FT MOD_RES 27
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9H019"
FT MOD_RES 38
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 100
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 107
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H019"
FT MOD_RES 221
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 222
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 235
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H019"
FT MOD_RES 258
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H019"
FT MOD_RES 270
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H019"
FT VAR_SEQ 94..289
FT /note="VMQRNASVPNLRGSEERLLALKKPALPALSRTTELQDELSHLRSQIAKIVAA
FT DAASASLTPDFFSSGSSNVSSPLPCFGSSLHSTTSFVISDITEETEVEVPELPTVPLLC
FT SASPECCKPEHKTTCSSSEEDDCISLSKASSFADMMGILKDFHRIKQSQDLSRSLLKEE
FT DPAVLISEVLRRKFALKEEDISRKGN -> EIFFFHGLSPSRKEK (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_034340"
FT CONFLICT 33
FT /note="P -> L (in Ref. 1; BAE27152)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="S -> Y (in Ref. 1; BAE27152)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="E -> K (in Ref. 1; BAE27152)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 289 AA; 31726 MW; 3CA0B5A8F33699B5 CRC64;
MEANVTIPIW QNKPHGAARS VVRRIGTNLP LKPCPRASFE TLPNISDLCL KDVPPVPTLA
DIAWIAADEE ETYARVRSDT RPLRHTWKPS PLIVMQRNAS VPNLRGSEER LLALKKPALP
ALSRTTELQD ELSHLRSQIA KIVAADAASA SLTPDFFSSG SSNVSSPLPC FGSSLHSTTS
FVISDITEET EVEVPELPTV PLLCSASPEC CKPEHKTTCS SSEEDDCISL SKASSFADMM
GILKDFHRIK QSQDLSRSLL KEEDPAVLIS EVLRRKFALK EEDISRKGN
