MFR1_SCHPO
ID MFR1_SCHPO Reviewed; 421 AA.
AC O94423;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 149.
DE RecName: Full=Meiotic fizzy-related protein 1;
GN Name=mfr1; ORFNames=SPBC1198.12, SPBC660.02;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11493649; DOI=10.1242/jcs.114.11.2135;
RA Blanco M.A., Pelloquin L., Moreno S.;
RT "Fission yeast mfr1 activates APC and coordinates meiotic nuclear division
RT with sporulation.";
RL J. Cell Sci. 114:2135-2143(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP INTERACTION WITH MES1.
RX PubMed=15791259; DOI=10.1038/nature03406;
RA Izawa D., Goto M., Yamashita A., Yamano H., Yamamoto M.;
RT "Fission yeast Mes1p ensures the onset of meiosis II by blocking
RT degradation of cyclin Cdc13p.";
RL Nature 434:529-533(2005).
CC -!- FUNCTION: Meiosis-specific activator of the anaphase promoting
CC complex/cyclosome (APC/C). Involved in cdc13 degradation.
CC {ECO:0000269|PubMed:11493649}.
CC -!- SUBUNIT: Interacts with mes1. {ECO:0000269|PubMed:15791259}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11493649}.
CC -!- SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CU329671; CAB91187.1; -; Genomic_DNA.
DR PIR; T40614; T40614.
DR RefSeq; NP_595081.1; NM_001020987.1.
DR AlphaFoldDB; O94423; -.
DR SMR; O94423; -.
DR BioGRID; 276581; 20.
DR ComplexPortal; CPX-766; Anaphase-Promoting Complex variant 4.
DR ELM; O94423; -.
DR IntAct; O94423; 2.
DR STRING; 4896.SPBC1198.12.1; -.
DR iPTMnet; O94423; -.
DR PaxDb; O94423; -.
DR EnsemblFungi; SPBC1198.12.1; SPBC1198.12.1:pep; SPBC1198.12.
DR GeneID; 2540043; -.
DR KEGG; spo:SPBC1198.12; -.
DR PomBase; SPBC1198.12; mfr1.
DR VEuPathDB; FungiDB:SPBC1198.12; -.
DR eggNOG; KOG0305; Eukaryota.
DR HOGENOM; CLU_014831_4_2_1; -.
DR InParanoid; O94423; -.
DR OMA; MDQDYES; -.
DR PhylomeDB; O94423; -.
DR PRO; PR:O94423; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0010997; F:anaphase-promoting complex binding; IPI:PomBase.
DR GO; GO:1990757; F:ubiquitin ligase activator activity; IDA:PomBase.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IDA:PomBase.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:1905786; P:positive regulation of anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0075296; P:positive regulation of ascospore formation; IMP:PomBase.
DR GO; GO:0051446; P:positive regulation of meiotic cell cycle; IMP:PomBase.
DR GO; GO:1905191; P:positive regulation of metaphase/anaphase transition of meiosis II; IMP:PomBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR033010; Cdc20/Fizzy.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19918; PTHR19918; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Meiosis; Nucleus; Reference proteome; Repeat; Sporulation; WD repeat.
FT CHAIN 1..421
FT /note="Meiotic fizzy-related protein 1"
FT /id="PRO_0000051077"
FT REPEAT 123..160
FT /note="WD 1"
FT REPEAT 164..203
FT /note="WD 2"
FT REPEAT 206..246
FT /note="WD 3"
FT REPEAT 247..286
FT /note="WD 4"
FT REPEAT 289..331
FT /note="WD 5"
FT REPEAT 333..374
FT /note="WD 6"
FT REPEAT 377..416
FT /note="WD 7"
FT REGION 79..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 85..101
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 421 AA; 47237 MW; 9B9D86FF08DF97D5 CRC64;
MGDRFIPIRN VSNEFNFSFQ SFKECVLSHG SNLRRKTSGT IQRQFMELLS MELFGSQASR
SRAFYYGEDK RKIEKKMLDT PDRKSYSLSP ISPQSQDMLR QPQKPKRAFP KTPYKILDAP
YLKNDFYLNL LDWGQSNVLA VGLASSIYLW SAASGKVVQL HDFGATNHVT SVLWTGKGTQ
LAVGTDSGVI YIWDIESTKS VRSLKGHSER VAALAWNDNT LTSGGKDEVI LHHDLRAPGC
CAEMMKVHEQ EICGLQWDRS LGQLASGGND NNLFVWDYRS SRPLHKFEEH TAAVKAIGWS
PHQRGILASG GGTIDRCLTI HNTLTGRLQN KLDTGSQVCN MAWSKTSNEI VTTHGFAKNQ
VSLWKYPSLK NIANLTAHTN RVLYLSMSPD GQSIVTGAGD ETLRFWKLFN KKPKEESTLI
R