ID   MFR5_PHOSM              Reviewed;         523 AA.
AC   A0A3G1DIQ9;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   13-FEB-2019, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=MFS-type transporter R5 {ECO:0000303|PubMed:27056201};
DE   AltName: Full=Squalestatin S1 biosynthesis cluster protein R5 {ECO:0000303|PubMed:27056201};
GN   Name=R5 {ECO:0000303|PubMed:27056201};
OS   Phoma sp. (strain ATCC 20986 / MF5453).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Didymellaceae; Phoma.
OX   NCBI_TaxID=1828523;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=27056201; DOI=10.1039/c6cc02130a;
RA   Bonsch B., Belt V., Bartel C., Duensing N., Koziol M., Lazarus C.M.,
RA   Bailey A.M., Simpson T.J., Cox R.J.;
RT   "Identification of genes encoding squalestatin S1 biosynthesis and in vitro
RT   production of new squalestatin analogues.";
RL   Chem. Commun. (Camb.) 52:6777-6780(2016).
CC   -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates
CC       the biosynthesis of squalestatin S1 (SQS1, also known as zaragozic acid
CC       A), a heavily oxidized fungal polyketide that offers potent cholesterol
CC       lowering activity by targeting squalene synthase (SS).
CC       {ECO:0000305|PubMed:27056201}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; KU946987; AMY15073.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3G1DIQ9; -.
DR   SMR; A0A3G1DIQ9; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
DR   PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..523
FT                   /note="MFS-type transporter R5"
FT                   /id="PRO_0000447832"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        291..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        319..339
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        408..428
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        443..463
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        470..490
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          19..42
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        22..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        35
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        94
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        143
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        235
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        250
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   523 AA;  56566 MW;  674077EDB9B95A27 CRC64;
     MNAATSITQA PVADVELKQL NEATAQRESA TNNPNDSSSI DERPIHSALS ESQRSALLAV
     ASFAAAISPA STTTYYPAIT TLARDLDVSI TQINLSISVY QIFQGLAPTV AAALSDRYGR
     RPVYLGCLFI NIAANLGLAR QENYTSLMVL RCLQSSSSSG TVALGQAVMD DLVTSEERGK
     YMAYLTLGLV MGPALGPLIG GLLSQYLGWR AIFWFLMILG GFFFVLTFTF FRETNRSIVG
     DGSVPPPKWN RSLVQILRKD KLVPNRESLQ KKRIGVNPLA SVQILCNKEN FIVCMYGALL
     FGGYASVISI FATQLEERYG YSQVQVGLCY LPFGVGSILS RWTAGKMIDW NFKREAEKQG
     LKIVKNRQQD LSQYDIEKAR LTISFPMIFA TCGFVVAYGW LMQYNTHVAS VLVIVFLIAN
     VFTGVLIANS ALLNDLNPGN GAALGAAMNL TRCLMGAGGV AAVTPLINKI GIGYTATATA
     GVWLAVLPAL YVVYSKGYTW RKAALRSSAQ GRGEGSRVAS PSP