ID   MFRE_WOLSU              Reviewed;         284 AA.
AC   Q7M825;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=8-methylmenaquinol:fumarate reductase membrane anchor subunit {ECO:0000303|PubMed:19170876};
DE            Short=MFR membrane anchor subunit {ECO:0000303|PubMed:19170876};
DE            EC=1.3.5.- {ECO:0000269|PubMed:19170876};
GN   Name=sdhE {ECO:0000303|PubMed:19170876};
GN   Synonyms=sdhC {ECO:0000312|EMBL:CAE10931.1};
GN   OrderedLocusNames=WS1922 {ECO:0000312|EMBL:CAE10931.1};
OS   Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC
OS   11488 / FDC 602W) (Vibrio succinogenes).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Wolinella.
OX   NCBI_TaxID=273121;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29543 / DSM 1740 / CCUG 13145 / JCM 31913 / LMG 7466 / NCTC
RC   11488 / FDC 602W;
RX   PubMed=14500908; DOI=10.1073/pnas.1932838100;
RA   Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O.,
RA   Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B.,
RA   Meyer F., Lederer H., Schuster S.C.;
RT   "Complete genome sequence and analysis of Wolinella succinogenes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, SUBUNIT, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 29543 / DSM 1740 / CCUG 13145 / JCM 31913 / LMG 7466 / NCTC
RC   11488 / FDC 602W;
RX   PubMed=19170876; DOI=10.1111/j.1365-2958.2008.06581.x;
RA   Juhnke H.D., Hiltscher H., Nasiri H.R., Schwalbe H., Lancaster C.R.;
RT   "Production, characterization and determination of the real catalytic
RT   properties of the putative 'succinate dehydrogenase' from Wolinella
RT   succinogenes.";
RL   Mol. Microbiol. 71:1088-1101(2009).
CC   -!- FUNCTION: Membrane anchor subunit of 8-methylmenaquinol:fumarate
CC       reductase (MFR), that catalyzes the reduction of fumarate using 8-
CC       methylmenaquinol-6 as electron donor. The complex shows no succinate
CC       oxidation activity. Is involved in anaerobic metabolism. SdhE likely
CC       contains the quinol/quinone binding site.
CC       {ECO:0000269|PubMed:19170876}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=8-methylmenaquinone-6 + succinate = 8-methylmenaquinol-6 +
CC         fumarate; Xref=Rhea:RHEA:51848, ChEBI:CHEBI:29806, ChEBI:CHEBI:30031,
CC         ChEBI:CHEBI:134356, ChEBI:CHEBI:134357;
CC         Evidence={ECO:0000269|PubMed:19170876};
CC   -!- SUBUNIT: The MFR complex is composed of three subunits: a flavoprotein
CC       (SdhA), an iron-sulfur protein (SdhB), and one hydrophobic anchor
CC       protein (SdhE). {ECO:0000269|PubMed:19170876}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:19170876}. Cell
CC       membrane {ECO:0000269|PubMed:19170876}; Peripheral membrane protein
CC       {ECO:0000305|PubMed:19170876}; Periplasmic side
CC       {ECO:0000269|PubMed:19170876}. Note=Membrane association is most likely
CC       mediated via amphipathic helices. {ECO:0000305|PubMed:19170876}.
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DR   EMBL; BX571662; CAE10931.1; -; Genomic_DNA.
DR   RefSeq; WP_011139714.1; NC_005090.1.
DR   AlphaFoldDB; Q7M825; -.
DR   SMR; Q7M825; -.
DR   STRING; 273121.WS1922; -.
DR   EnsemblBacteria; CAE10931; CAE10931; WS1922.
DR   KEGG; wsu:WS1922; -.
DR   eggNOG; COG2048; Bacteria.
DR   HOGENOM; CLU_052147_1_0_7; -.
DR   OMA; SCCGASH; -.
DR   OrthoDB; 647535at2; -.
DR   Proteomes; UP000000422; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004017; Cys_rich_dom.
DR   Pfam; PF02754; CCG; 2.
PE   1: Evidence at protein level;
KW   Cell membrane; Electron transport; Membrane; Oxidoreductase; Periplasm;
KW   Reference proteome; Transport.
FT   CHAIN           1..284
FT                   /note="8-methylmenaquinol:fumarate reductase membrane
FT                   anchor subunit"
FT                   /id="PRO_0000437542"
SQ   SEQUENCE   284 AA;  30655 MW;  8FCC234FF78C0699 CRC64;
     MQKEFAFFPG CVLSQAAIES KKSIEAIAPV LGIKLREIEG WSCCGASQAQ CVDPLATLVA
     NARNLALAEQ MNLPVLTTCS TCLLMLTRAK AELDRGAKDQ INSFLAKGNM SYQGTSEVTS
     LLWVLAQNVE ELKSKVKKPL SNLKVAVFYG CHSLRPEKDL GFESSTNPTS FETIVKALGA
     QVVPFEKRLN CCGFHAVYPA ESSAMKMTSG IINTAAKSEA HCVVTPCPLC QMQLDIYQED
     AQKIAKSKER VPVLHLSQLV GLALGIPAKE LGLNHNVIDA TKLG