ID   MFRN2_DANRE             Reviewed;         376 AA.
AC   Q7T292;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Mitoferrin-2;
DE   AltName: Full=Mitochondrial iron transporter 2;
DE   AltName: Full=Solute carrier family 25 member 28;
GN   Name=slc25a28; Synonyms=mfrn2; ORFNames=zgc:64212;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=16511496; DOI=10.1038/nature04512;
RA   Shaw G.C., Cope J.J., Li L., Corson K., Hersey C., Ackermann G.E.,
RA   Gwynn B., Lambert A.J., Wingert R.A., Traver D., Trede N.S., Barut B.A.,
RA   Zhou Y., Minet E., Donovan A., Brownlie A., Balzan R., Weiss M.J.,
RA   Peters L.L., Kaplan J., Zon L.I., Paw B.H.;
RT   "Mitoferrin is essential for erythroid iron assimilation.";
RL   Nature 440:96-100(2006).
CC   -!- FUNCTION: Mitochondrial iron transporter that mediates iron uptake.
CC       Probably required for heme synthesis of hemoproteins and Fe-S cluster
CC       assembly in non-erythroid cells. The iron delivered into the
CC       mitochondria, presumably as Fe(2+), is then probably delivered to
CC       ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX
CC       to make heme (Probable). {ECO:0000305|PubMed:16511496}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels throughout the
CC       whole embryo at 24 hpf. {ECO:0000269|PubMed:16511496}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
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DR   EMBL; BC054641; AAH54641.1; -; mRNA.
DR   AlphaFoldDB; Q7T292; -.
DR   SMR; Q7T292; -.
DR   STRING; 7955.ENSDARP00000069436; -.
DR   PaxDb; Q7T292; -.
DR   ZFIN; ZDB-GENE-040426-2125; slc25a28.
DR   eggNOG; KOG0760; Eukaryota.
DR   HOGENOM; CLU_015166_3_1_1; -.
DR   InParanoid; Q7T292; -.
DR   PhylomeDB; Q7T292; -.
DR   TreeFam; TF314118; -.
DR   PRO; PR:Q7T292; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031966; C:mitochondrial membrane; IBA:GO_Central.
DR   GO; GO:0015093; F:ferrous iron transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0048250; P:iron import into the mitochondrion; IBA:GO_Central.
DR   GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.40.10; -; 2.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   Pfam; PF00153; Mito_carr; 3.
DR   SUPFAM; SSF103506; SSF103506; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   2: Evidence at transcript level;
KW   Ion transport; Iron; Iron transport; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..376
FT                   /note="Mitoferrin-2"
FT                   /id="PRO_0000235257"
FT   TRANSMEM        77..96
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..157
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..194
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..251
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        266..285
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..353
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          75..163
FT                   /note="Solcar 1"
FT   REPEAT          173..257
FT                   /note="Solcar 2"
FT   REPEAT          264..359
FT                   /note="Solcar 3"
SQ   SEQUENCE   376 AA;  41523 MW;  C3BDE7DC45BFE0E2 CRC64;
     MTADTSSGDA AVAGASAGAE IHWFGGRFWG VSESLVGTLT PRISGEPDLH VGHFYGSQEA
     SDLSEPDYEG LPQGASTSTH MLAGAVAGIM EHCLMFPIDC VKTRMQSLQP EPAARYRNVM
     DALWRIMRTE GIWRPIRGLN ITAVGAGPAH ALYFACYERL KKVLSDIIHP GANSHLANGA
     AGCVATLLHD AAMNPTEVVK QRMQMYNSPY RSVLDCMRCV WQREGALAFY RSYTTQLTMN
     VPFQALHFMT YEYLQELLNP QRHYNPSSHM VSGALAGAIA AAATTPLDVC KTLLNTQESL
     AVDSVSRSGR HITGLGHAFR TVYRLGGLPA YFKGVQARVI YQMPSTAISW SVYEFFKYMI
     TKHQHEKRRI QRDAEK