MFRN_DROME
ID MFRN_DROME Reviewed; 379 AA.
AC Q9VAY3; Q9NHY6;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Mitoferrin;
DE Short=dmfrn;
GN Name=mfrn; ORFNames=CG4963;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Canton-S; TISSUE=Embryo;
RX PubMed=9275195; DOI=10.1073/pnas.94.18.9746;
RA Schmid K.J., Tautz D.;
RT "A screen for fast evolving genes from Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:9746-9750(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19453295; DOI=10.1042/bj20082231;
RA Metzendorf C., Wu W., Lind M.I.;
RT "Overexpression of Drosophila mitoferrin in l(2)mbn cells results in
RT dysregulation of Fer1HCH expression.";
RL Biochem. J. 421:463-471(2009).
CC -!- FUNCTION: Mitochondrial iron transporter that mediates iron uptake.
CC Probably required for heme synthesis of hemoproteins and Fe-S cluster
CC assembly. {ECO:0000269|PubMed:19453295}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:19453295}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19453295}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF73387.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF217402; AAF73387.1; ALT_FRAME; mRNA.
DR EMBL; AE014297; AAF56764.1; -; Genomic_DNA.
DR EMBL; AY060268; AAL25307.1; -; mRNA.
DR RefSeq; NP_651600.1; NM_143343.3.
DR AlphaFoldDB; Q9VAY3; -.
DR SMR; Q9VAY3; -.
DR BioGRID; 68234; 10.
DR IntAct; Q9VAY3; 1.
DR STRING; 7227.FBpp0084635; -.
DR PaxDb; Q9VAY3; -.
DR PRIDE; Q9VAY3; -.
DR DNASU; 43353; -.
DR EnsemblMetazoa; FBtr0085266; FBpp0084635; FBgn0039561.
DR GeneID; 43353; -.
DR KEGG; dme:Dmel_CG4963; -.
DR UCSC; CG4963-RA; d. melanogaster.
DR CTD; 43353; -.
DR FlyBase; FBgn0039561; mfrn.
DR VEuPathDB; VectorBase:FBgn0039561; -.
DR eggNOG; KOG0760; Eukaryota.
DR GeneTree; ENSGT00940000169345; -.
DR HOGENOM; CLU_015166_3_1_1; -.
DR InParanoid; Q9VAY3; -.
DR OMA; KVMNPSQ; -.
DR OrthoDB; 1056670at2759; -.
DR PhylomeDB; Q9VAY3; -.
DR BioGRID-ORCS; 43353; 1 hit in 3 CRISPR screens.
DR ChiTaRS; mfrn; fly.
DR GenomeRNAi; 43353; -.
DR PRO; PR:Q9VAY3; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039561; Expressed in adult Malpighian tubule (Drosophila) and 25 other tissues.
DR Genevisible; Q9VAY3; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
DR GO; GO:0031966; C:mitochondrial membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0015093; F:ferrous iron transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0048250; P:iron import into the mitochondrion; IDA:UniProtKB.
DR GO; GO:0060586; P:multicellular organismal iron ion homeostasis; IMP:FlyBase.
DR GO; GO:0048515; P:spermatid differentiation; IMP:FlyBase.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Ion transport; Iron; Iron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..379
FT /note="Mitoferrin"
FT /id="PRO_0000235262"
FT TRANSMEM 14..33
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..92
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..124
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..183
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..217
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..275
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 12..98
FT /note="Solcar 1"
FT REPEAT 105..189
FT /note="Solcar 2"
FT REPEAT 196..281
FT /note="Solcar 3"
FT REGION 357..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 84
FT /note="A -> T (in Ref. 1; AAF73387)"
FT /evidence="ECO:0000305"
FT CONFLICT 312
FT /note="D -> E (in Ref. 1; AAF73387)"
FT /evidence="ECO:0000305"
FT CONFLICT 334
FT /note="S -> C (in Ref. 1; AAF73387)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="V -> F (in Ref. 1; AAF73387)"
FT /evidence="ECO:0000305"
FT CONFLICT 376
FT /note="G -> A (in Ref. 1; AAF73387)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 379 AA; 41795 MW; F6A20BABA35F5C2E CRC64;
MNIDDYESLP TTSVGVNMTA GAIAGVLEHV VMYPLDSVKT RMQSLSPPTK NMNIVSTLRT
MITREGLLRP IRGASAVVLG AGPAHSLYFA AYEMTKELTA KFTSVRNLNY VISGAVATLI
HDAISSPTDV IKQRMQMYNS PYTSVVSCVR DIYKREGFKA FYRAYGTQLV MNLPYQTIHF
TTYEFFQNKM NLERKYNPPV HMAAGAAAGA CAAAVTTPLD VIKTLLNTQE TGLTRGMIEA
SRKIYHMAGP LGFFRGTTAR VLYSMPATAI CWSTYEFFKF YLCGLDADQY KSSITGSSEP
RKADYVLPRT TDEEQIDQER EAAKEKDTTA TLHSAPTSVN ASGAIKTVCE LSTRPAGPTI
NLHTRHTDVK SPYERGFST
