MFS19_ALTAL
ID MFS19_ALTAL Reviewed; 489 AA.
AC A0A4Q4NJ90;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 31-JUL-2019, sequence version 1.
DT 25-MAY-2022, entry version 11.
DE RecName: Full=MFS-type transporter MFS19 {ECO:0000303|PubMed:28060864};
GN Name=MFS19 {ECO:0000303|PubMed:28060864}; ORFNames=AA0117_g5322;
OS Alternaria alternata (Alternaria rot fungus) (Torula alternata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC Alternaria sect. Alternaria; Alternaria alternata complex.
OX NCBI_TaxID=5599;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FERA 1177;
RX PubMed=32038562; DOI=10.3389/fmicb.2019.03124;
RA Armitage A.D., Cockerton H.M., Sreenivasaprasad S., Woodhall J., Lane C.R.,
RA Harrison R.J., Clarkson J.P.;
RT "Genomics evolutionary history and diagnostics of the Alternaria alternata
RT species group including apple and asian pear pathotypes.";
RL Front. Microbiol. 10:3124-3124(2019).
RN [2]
RP INDUCTION.
RX PubMed=20978890; DOI=10.1007/s00284-010-9795-y;
RA Lin C.H., Yang S.L., Chung K.R.;
RT "Cellular responses required for oxidative stress tolerance, colonization,
RT and lesion formation by the necrotrophic fungus Alternaria alternata in
RT citrus.";
RL Curr. Microbiol. 62:807-815(2011).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=28060864; DOI=10.1371/journal.pone.0169103;
RA Chen L.H., Tsai H.C., Yu P.L., Chung K.R.;
RT "A Major Facilitator Superfamily Transporter-Mediated Resistance to
RT Oxidative Stress and Fungicides Requires Yap1, Skn7, and MAP Kinases in the
RT Citrus Fungal Pathogen Alternaria alternata.";
RL PLoS ONE 12:e0169103-e0169103(2017).
CC -!- FUNCTION: MFS-type efflux pump involved in the modulation
CC susceptibility to various compounds including cumyl hydroperoxide,
CC potassium superoxide, many singlet oxygen-generating compounds (eosin
CC Y, rose Bengal, hematoporphyrin, methylene blue, and cercosporin), and
CC the cell wall biosynthesis inhibitor Congo red (PubMed:28060864).
CC Involved in oxidative stress tolerance, colonization, and lesion
CC formation (PubMed:28060864). {ECO:0000269|PubMed:28060864}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:28060864};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Expression is regulated by the Yap1 transcription activator,
CC the Hog1 and Fus3 mitogen-activated protein (MAP) kinases, the two
CC component histidine kinase, and the Skn7 response regulator.
CC {ECO:0000269|PubMed:20978890, ECO:0000269|PubMed:28060864}.
CC -!- DISRUPTION PHENOTYPE: Displays profound hypersensitivities to cumyl
CC hydroperoxide, potassium superoxide, many singlet oxygen-generating
CC compounds (eosin Y, rose Bengal, hematoporphyrin, methylene blue, and
CC cercosporin), and the cell wall biosynthesis inhibitor, Congo red
CC (PubMed:28060864). Also increases sensitivity to copper ions,
CC clotrimazole, fludioxonil, and kocide fungicides, 2-chloro-5-
CC hydroxypyridine (CHP), and 2,3,5-triiodobenzoic acid (TIBA)
CC (PubMed:28060864). Leads to smaller necrotic lesions on leaves of a
CC susceptible citrus cultivar (PubMed:28060864).
CC {ECO:0000269|PubMed:28060864}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; PDXD01000010; RYN76906.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4Q4NJ90; -.
DR Proteomes; UP000291422; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Transmembrane; Transmembrane helix;
KW Transport; Virulence.
FT CHAIN 1..489
FT /note="MFS-type transporter MFS19"
FT /id="PRO_0000452754"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 454..474
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 489 AA; 53131 MW; AF1530465A854246 CRC64;
MAHSTAGDRD PEVGSEQHSS IAQLHTESMS DPWGDSNSPE NPLNWPAPKK NFHVAIVSIF
TLTANLAATM FAPGAPQLAK EFNITNSTVE AMTVSLYVLG FAFGPLLLAP LSELYGRLII
YNACNAVYIA FTVGCAFSTN VSMFLVFRFL CGCAASGPMS IGGGTVADIT PQEERGKAMA
LFAMGPLLGP VLGPIIGGYV SQYTNWRWTF RIILIMSGII GLATMFFMRE TNAAVLLRRK
AKRSPKDAEG MELELDKTKK ETPSQVLVRA ITRPFKMLLF SPIVLLISLY TGVLFGLIFL
LFTTFPTVFQ GVYGFDEGTS GLAYLGLGIG MFLGLVVFSI LSDKLLGQKQ GGTVSKPEQR
LILMKWFGPI TPLGCFMYGW SAYHHVHWIV PILGTSIIGF GSLFVVIPGQ IYLVDSFGAE
AAASALAANL LVRSPFGAFL GLVAAPLYDR LSLGWGNSVL GFITLAFTPV PWLFYRYGET
LRTRFVVKL
