MFS4B_HUMAN
ID MFS4B_HUMAN Reviewed; 518 AA.
AC Q5TF39; A8K9M0; Q8IYB6; Q96PW9; Q9P0D6;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Sodium-dependent glucose transporter 1 {ECO:0000250|UniProtKB:Q80T22};
DE AltName: Full=Major facilitator superfamily domain-containing protein 4B {ECO:0000312|HGNC:HGNC:21053};
GN Name=MFSD4B {ECO:0000312|HGNC:HGNC:21053}; Synonyms=KIAA1919, NAGLT1;
GN ORFNames=HSPC100;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PRO-93.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-518.
RC TISSUE=Brain;
RX PubMed=11572484; DOI=10.1093/dnares/8.4.179;
RA Nagase T., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XXI. The
RT complete sequences of 60 new cDNA clones from brain which code for large
RT proteins.";
RL DNA Res. 8:179-187(2001).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 423-518.
RC TISSUE=Umbilical cord blood;
RA Zhang Q.-H., Ye M., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L.,
RA Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z.;
RT "Human partial CDS cloned from CD34+ stem cells.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May function as a sodium-dependent glucose transporter.
CC Potential channels for urea in the inner medulla of kidney.
CC {ECO:0000250|UniProtKB:Q80T22}.
CC -!- INTERACTION:
CC Q5TF39; O43169: CYB5B; NbExp=3; IntAct=EBI-11922631, EBI-1058710;
CC Q5TF39; Q96PD2-2: DCBLD2; NbExp=3; IntAct=EBI-11922631, EBI-12135455;
CC Q5TF39; Q6ZSS7: MFSD6; NbExp=3; IntAct=EBI-11922631, EBI-2858252;
CC Q5TF39; Q6UX27-3: VSTM1; NbExp=3; IntAct=EBI-11922631, EBI-12190699;
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000250|UniProtKB:Q80T22}; Multi-pass membrane protein
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; AK292735; BAF85424.1; -; mRNA.
DR EMBL; AL080317; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471051; EAW48296.1; -; Genomic_DNA.
DR EMBL; BC036115; AAH36115.1; -; mRNA.
DR EMBL; AB067506; BAB67812.1; -; mRNA.
DR EMBL; AF161363; AAF28923.1; -; mRNA.
DR CCDS; CCDS5090.1; -.
DR RefSeq; NP_699200.2; NM_153369.2.
DR AlphaFoldDB; Q5TF39; -.
DR SMR; Q5TF39; -.
DR BioGRID; 124873; 19.
DR IntAct; Q5TF39; 10.
DR STRING; 9606.ENSP00000357840; -.
DR TCDB; 2.A.1.7.27; the major facilitator superfamily (mfs).
DR iPTMnet; Q5TF39; -.
DR PhosphoSitePlus; Q5TF39; -.
DR BioMuta; MFSD4B; -.
DR DMDM; 74746358; -.
DR EPD; Q5TF39; -.
DR jPOST; Q5TF39; -.
DR MassIVE; Q5TF39; -.
DR MaxQB; Q5TF39; -.
DR PaxDb; Q5TF39; -.
DR PeptideAtlas; Q5TF39; -.
DR PRIDE; Q5TF39; -.
DR ProteomicsDB; 65070; -.
DR Antibodypedia; 32375; 27 antibodies from 15 providers.
DR DNASU; 91749; -.
DR Ensembl; ENST00000671876.2; ENSP00000500934.1; ENSG00000173214.7.
DR Ensembl; ENST00000671883.1; ENSP00000499903.1; ENSG00000173214.7.
DR Ensembl; ENST00000672191.1; ENSP00000500175.1; ENSG00000173214.7.
DR Ensembl; ENST00000672750.1; ENSP00000500686.1; ENSG00000173214.7.
DR GeneID; 91749; -.
DR KEGG; hsa:91749; -.
DR MANE-Select; ENST00000671876.2; ENSP00000500934.1; NM_153369.4; NP_699200.2.
DR UCSC; uc003puv.5; human.
DR CTD; 91749; -.
DR DisGeNET; 91749; -.
DR GeneCards; MFSD4B; -.
DR HGNC; HGNC:21053; MFSD4B.
DR HPA; ENSG00000173214; Low tissue specificity.
DR MIM; 617331; gene.
DR neXtProt; NX_Q5TF39; -.
DR OpenTargets; ENSG00000173214; -.
DR PharmGKB; PA128394744; -.
DR VEuPathDB; HostDB:ENSG00000173214; -.
DR eggNOG; ENOG502R5UW; Eukaryota.
DR GeneTree; ENSGT00530000063320; -.
DR HOGENOM; CLU_028923_1_0_1; -.
DR InParanoid; Q5TF39; -.
DR OMA; EPTAEIY; -.
DR OrthoDB; 1122917at2759; -.
DR PhylomeDB; Q5TF39; -.
DR TreeFam; TF314613; -.
DR PathwayCommons; Q5TF39; -.
DR Reactome; R-HSA-189200; Cellular hexose transport.
DR SignaLink; Q5TF39; -.
DR BioGRID-ORCS; 91749; 6 hits in 1065 CRISPR screens.
DR ChiTaRS; MFSD4B; human.
DR GenomeRNAi; 91749; -.
DR Pharos; Q5TF39; Tdark.
DR PRO; PR:Q5TF39; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q5TF39; protein.
DR Bgee; ENSG00000173214; Expressed in renal medulla and 183 other tissues.
DR ExpressionAtlas; Q5TF39; baseline and differential.
DR Genevisible; Q5TF39; HS.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005355; F:glucose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium transport; Sugar transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..518
FT /note="Sodium-dependent glucose transporter 1"
FT /id="PRO_0000294510"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 414..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 93
FT /note="S -> P (in dbSNP:rs17853558)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_033192"
SQ SEQUENCE 518 AA; 56218 MW; FCB02A5E428BD0F5 CRC64;
MLCASFLGLG LSVAIVGPTF QDLATNVNRN ISSLSFIFVG RALGYLSGSV IGGFLVDVMN
YFLLLGISMS ATTVGLYLVP FCKTAILLTV MMSIFGVSIG ILDTGGNVLI LAIWGDKGAP
HMQALHFSFA LGAFLAPLLA KLALGPTASA ENHTESDFHP ALNQSSDADS EALFGVPNDK
NLLWAYAVIG TYMFLVSVIF FCLFLKNSSK QEKARASAET FRRAKYHNAL LCLLFLFFFF
YVGAEVTYGS YVFSFATTHA GMKESEAAGL NSIFWGTFAA CRGLAIFFAT CLQPGTMIVL
SNIGSLTSSL FLVLFDKNPI CLWIATSVYG ASMATTFPSG VSWIEQYTTI HGKSAAFFVI
GASLGEMAIP AVIGILQGKY PDLPVVLYTS LGASIATGIL FPVLYKLATS PLDRQRKEDR
KSEDQKALLS SSGLNEYEEE NEEEDAEKWN EMDFEMIETN DTMRHSIIET SRSSLTEPTA
EVYNQYPSNA LVFESSPFNT GSAHVKHLPE TRTKGTNV
