MFS54_ALTAL
ID MFS54_ALTAL Reviewed; 538 AA.
AC A0A4Q4NMP3;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 31-JUL-2019, sequence version 1.
DT 25-MAY-2022, entry version 12.
DE RecName: Full=MFS-type transporter MFS54 {ECO:0000303|PubMed:30279684};
GN Name=MFS54 {ECO:0000303|PubMed:30279684}; ORFNames=AA0117_g3624;
OS Alternaria alternata (Alternaria rot fungus) (Torula alternata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC Alternaria sect. Alternaria; Alternaria alternata complex.
OX NCBI_TaxID=5599;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FERA 1177;
RX PubMed=32038562; DOI=10.3389/fmicb.2019.03124;
RA Armitage A.D., Cockerton H.M., Sreenivasaprasad S., Woodhall J., Lane C.R.,
RA Harrison R.J., Clarkson J.P.;
RT "Genomics evolutionary history and diagnostics of the Alternaria alternata
RT species group including apple and asian pear pathotypes.";
RL Front. Microbiol. 10:3124-3124(2019).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=30279684; DOI=10.3389/fmicb.2018.02229;
RA Lin H.C., Yu P.L., Chen L.H., Tsai H.C., Chung K.R.;
RT "A Major Facilitator Superfamily Transporter Regulated by the Stress-
RT Responsive Transcription Factor Yap1 Is Required for Resistance to
RT Fungicides, Xenobiotics, and Oxidants and Full Virulence in Alternaria
RT alternata.";
RL Front. Microbiol. 9:2229-2229(2018).
CC -!- FUNCTION: MFS-type efflux pump involved in the modulation
CC susceptibility to various compounds including the xenobiotics 2,3,5-
CC triiodobenzoic acid (TIBA) and 2-chloro-5-hydroxypyridine (CHP),
CC CuCl(2) several fungicides (clotrimazole, fludioxonil, vinclozolin, and
CC iprodione), potassium superoxide KO(2), and the singlet oxygen-
CC generating compound hematoporphyrin. {ECO:0000269|PubMed:30279684}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:30279684};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Expression is down-regulated in the presence of TIBA or CHP,
CC and induced in the presence of H(2)O(2), clotrimazole, or vinclozolin
CC (PubMed:30279684). Expression is regulated by the stress-responsive
CC transcription regulator Yap1 but not the Hog1 MAP kinase
CC (PubMed:30279684). {ECO:0000269|PubMed:30279684}.
CC -!- DISRUPTION PHENOTYPE: Leads to a decreased production of conidia and a
CC reduced radial growth by about 20% on potato dextrose agar plates
CC (PubMed:30279684). Leads also to increased sensitivity to the
CC xenobiotics 2,3,5-triiodobenzoic acid (TIBA) and 2-chloro-5-
CC hydroxypyridine (CHP), CuCl(2) and several fungicides including
CC clotrimazole, fludioxonil, vinclozolin, and iprodione
CC (PubMed:30279684). Increases also the sensitivity to potassium
CC superoxide KO(2) and the singlet oxygen-generating compound
CC hematoporphyrin (PubMed:30279684). Does not affect the sensitivity to
CC H(2)O(2), singlet oxygen-generating compounds rose Bengal and eosin Y,
CC or to the cell wall disturbing compound Congo red (PubMed:30279684).
CC {ECO:0000269|PubMed:30279684}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; PDXD01000005; RYN79724.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4Q4NMP3; -.
DR SMR; A0A4Q4NMP3; -.
DR Proteomes; UP000291422; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..538
FT /note="MFS-type transporter MFS54"
FT /id="PRO_0000452753"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 507..527
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 538 AA; 57832 MW; DB0C920D9C6234E1 CRC64;
MKEGVVTPPD SEKDLQNVEG SLNTEEEKAE EAHIQLSKGR FVLVLVGLVL AIFLASLDFT
IISTAIPKIT DEFHSLQDIG WYGSAFFITT AATTAHWGRL YTFFPLKWTY LTSIFFFELG
SVICGAAPSS TALIIGRAIC GIGAAGLFSG SYTIIAVLVP SADRPKYSGL MGASYGLASV
VGPLIGGAFT SHVSWRWCFY INLPVGGVSC LFILLFFANE PPKSRPDWRG MLRQLDMLGL
VLIVGAITCF ILALQWGGVS KAWDSGAVIG TLVAFVVCMI LFVIEQWWLG ENAMVHSVMI
KRRTIWVGSM FSFLINSAFL VTFYYLPIYF QSVQGVSAST SGVRTVPFIL AVTFCVVIVG
QIITKTGYAF PWMIVGAAIT TIGSGMIYTF DTHSPAGKWI GYQILCGIGV GVSFQVPVML
IQATTKDADV PLATATLLFI QTLGGAFGVS SAQAAFQNTL LKQLAITAPG LNPQIVLDAG
ASELKRVIPE QFLQGVLEAY VSGFRECLIV GIAFGGAAFL AAFGFRFTNI KRTAAAEA
