MFS55_MYCS2
ID MFS55_MYCS2 Reviewed; 560 AA.
AC A0QWU7;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Probable triacylglyceride transporter MSMEG_3069/MSMEI_2992 {ECO:0000250|UniProtKB:P9WJY3};
DE AltName: Full=MFS-type drug efflux transporter P55 {ECO:0000303|PubMed:18156250};
GN OrderedLocusNames=MSMEG_3069, MSMEI_2992;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, ACTIVITY REGULATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18156250; DOI=10.1128/jb.01046-07;
RA Farrow M.F., Rubin E.J.;
RT "Function of a mycobacterial major facilitator superfamily pump requires a
RT membrane-associated lipoprotein.";
RL J. Bacteriol. 190:1783-1791(2008).
CC -!- FUNCTION: In association with lipoprotein LprG probably transports
CC triacyglycerides (TAG) across the inner cell membrane into the
CC periplasm; TAG probably regulates lipid metabolism and growth
CC regulation (By similarity). Confers resistance to ethidium bromide,
CC possibly acting as an efflux pump, requires LprG lipoprotein for normal
CC function (PubMed:18156250). Export of ethidium bromide can be
CC complemented by the equivalent operon from M.tuberculosis (lprG-
CC Rv1410c) (PubMed:18156250). {ECO:0000250|UniProtKB:P9WJY3,
CC ECO:0000269|PubMed:18156250}.
CC -!- ACTIVITY REGULATION: Inhibited by reserpine (PubMed:18156250).
CC {ECO:0000269|PubMed:18156250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Double deletions of lprG-MSMEG_3069/MSMEI_2992
CC operon are more sensitive to ethidium bromide; restoration of wild-type
CC growth is conferred by the M.tuberculosis operonells display abnormal
CC colony morphology and are defective for sliding motility under cabon-
CC limiting conditions (PubMed:18156250). {ECO:0000269|PubMed:18156250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; CP000480; ABK73093.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP39456.1; -; Genomic_DNA.
DR RefSeq; WP_011728795.1; NZ_CP009494.1.
DR RefSeq; YP_887385.1; NC_008596.1.
DR AlphaFoldDB; A0QWU7; -.
DR SMR; A0QWU7; -.
DR STRING; 246196.MSMEI_2992; -.
DR PRIDE; A0QWU7; -.
DR EnsemblBacteria; ABK73093; ABK73093; MSMEG_3069.
DR EnsemblBacteria; AFP39456; AFP39456; MSMEI_2992.
DR KEGG; msg:MSMEI_2992; -.
DR KEGG; msm:MSMEG_3069; -.
DR PATRIC; fig|246196.19.peg.3030; -.
DR eggNOG; COG0477; Bacteria.
DR OMA; AGYWMTP; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Lipid transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..560
FT /note="Probable triacylglyceride transporter
FT MSMEG_3069/MSMEI_2992"
FT /id="PRO_0000438415"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 519..560
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 560 AA; 58286 MW; 7F3724F1626A6AD8 CRC64;
MSSRGNRNIA ISAGSLAVLL GALDTYVVIT IIVDIMADVG IAINQIQQVT PIITGYLLGY
IAAMPLLGRA SDRFGRKMLI QVGLAGFAVG SVVTALSSDL TMLVIGRIIQ GSASGALLPV
TLALAADLWS ARSRASVLGG VGAAQELGAV LGPMYGIALV WLFNHWQAVF WVNVPLAVIA
MVMIHFSLPA RQQVDEPERV DVIGGVLLAI ALGLTVVGLY NPEPDGKQVL PSWGLPVLAG
ALVAAVAFFA WEKVAKTRLI DPAGVRFRPF LAALAASLCA GAALMVTLVN VELFGQGVLG
QDQDHAAFLL LRFLIALPIG ALIGGWLATR IGDRLVVLIG LLIAAGGFVL ISHWSVDVLA
DRHNLGLFTL PVLDTDLAIV GLGLGLVIGP LTSATLRAVP AAEHGIASAA VVVARMIGML
IGIAALGAWG FYRFNQHLAT LAARAAGDAG SPMSLAERLT AQAVRYREAY VMMYGDIFLS
AAVVCVIGAL LGLLISGKHE HAEEFEPAYA PTYGGGGAID PYDAGDADDA PTEMLDLPTQ
VLSAPPSDPG DERPGRHRAP
