ID   MFSD1_BOVIN             Reviewed;         468 AA.
AC   Q1JQC1;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Major facilitator superfamily domain-containing protein 1;
GN   Name=MFSD1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Lysosomal transporter which is essential for liver
CC       homeostasis. Required to maintain stability and lysosomal localization
CC       of GLMP. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SUBUNIT: Homodimer. Interacts with lysosomal protein GLMP (via lumenal
CC       domain); the interaction starts while both proteins are still in the
CC       endoplasmic reticulum and is required for stability and lysosomal
CC       localization of MFSD1. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q9DC37};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- DOMAIN: The dileucine internalization motif is required for lysosomal
CC       localization. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- PTM: Not N-glycosylated. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC116065; AAI16066.1; -; mRNA.
DR   RefSeq; NP_001068819.1; NM_001075351.1.
DR   AlphaFoldDB; Q1JQC1; -.
DR   SMR; Q1JQC1; -.
DR   STRING; 9913.ENSBTAP00000012206; -.
DR   PaxDb; Q1JQC1; -.
DR   GeneID; 508337; -.
DR   KEGG; bta:508337; -.
DR   CTD; 64747; -.
DR   eggNOG; KOG4686; Eukaryota.
DR   HOGENOM; CLU_024694_3_1_1; -.
DR   InParanoid; Q1JQC1; -.
DR   OrthoDB; 941048at2759; -.
DR   TreeFam; TF323603; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0061462; P:protein localization to lysosome; ISS:UniProtKB.
DR   GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Lysosome; Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..468
FT                   /note="Major facilitator superfamily domain-containing
FT                   protein 1"
FT                   /id="PRO_0000273381"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        173..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        270..290
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        307..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        364..384
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..415
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        421..441
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           11..12
FT                   /note="Dileucine internalization motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q9DC37"
SQ   SEQUENCE   468 AA;  51538 MW;  70EB5B02C797B84D CRC64;
     MEDEEEEARA LLPGGSDEAG RETRAPPAAS GALQALCDPS HLAHRLVVLL LMCFLGFGSY
     FCYDNPAALQ IQVKRDMQVN TTKFMLLYAW YSWPNVVLCF FGGFLIDRVF GIRWGTIIFS
     CFVCIGQVVF ALGGIFNAFW LMELGRFVFG IGGESLAVAQ NTYAVSWFKG KELNLVFGLQ
     LSMARIGSTV NMNLMGWLYS KVEASLGSAG HTTLGVTLMI GGITCILSLV CALALAYLDQ
     RAERILHKEQ GKTGEVIKLT DVKDFSLPLW LIFIICVCYY VAIFPFIGLV KVFFTEKFGF
     SSQAASAINS VVYVISAPMS PIFGLLVDKT GKNIIWVLCA VVTTLASHIM LAFTLWNPWI
     AMCLLGLSYS LLACALWPMV AFVVPEHQLG TAYGFMQSIQ NLGLAVISII AGMILDTRGY
     LFLEVFFIAC VSLSLLSVVL LYMVNHAQGG NLNYSAKKRE EMKLSHEE