ID   MFSD1_DANRE             Reviewed;         461 AA.
AC   Q32LQ6;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Major facilitator superfamily domain-containing protein 1;
GN   Name=mfsd1; ORFNames=zgc:123187;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Lysosomal transporter which is essential for liver
CC       homeostasis. Required to maintain stability and lysosomal localization
CC       of GLMP. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q9DC37};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- DOMAIN: The dileucine internalization motif is required for lysosomal
CC       localization. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- PTM: Not N-glycosylated. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC109468; AAI09469.1; -; mRNA.
DR   RefSeq; NP_001032503.1; NM_001037426.1.
DR   AlphaFoldDB; Q32LQ6; -.
DR   SMR; Q32LQ6; -.
DR   GeneID; 641486; -.
DR   KEGG; dre:641486; -.
DR   CTD; 64747; -.
DR   ZFIN; ZDB-GENE-051120-153; mfsd1.
DR   InParanoid; Q32LQ6; -.
DR   OrthoDB; 941048at2759; -.
DR   PhylomeDB; Q32LQ6; -.
DR   PRO; PR:Q32LQ6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0061462; P:protein localization to lysosome; ISS:UniProtKB.
DR   GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Lysosome; Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..461
FT                   /note="Major facilitator superfamily domain-containing
FT                   protein 1"
FT                   /id="PRO_0000273386"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        302..322
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        359..379
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        390..410
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        416..436
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           10..11
FT                   /note="Dileucine internalization motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q9DC37"
SQ   SEQUENCE   461 AA;  50995 MW;  4350979028374CCD CRC64;
     MADREEHQGL LDGDRDEDEG DKAPSVHLNP ICDPNHLLHR ILVLIFMCFL GFGSYFCYDN
     PAALQSQVIQ DMNLNTASFM QLYAWYSWPN VVLCFLGGFL LDRVFGIRLG TVIFSLFVLV
     GQIIFAAGAL ANHFWLMNVG RFVFGIGGES LAVAQNTYAV NWFKGKELNL VFGLQLSMAR
     LGSTVNMNVI GWVYGRIQMS MGSAGPTTLG ITLMIAASTC LFSLICALVL GFLDKRAERI
     LNKEQGKTGE VIKLTDVKDF SVSLWLIFII CVAYYVAIFP FIGLGQVFFI EKFGFTPVQA
     RAINSVVYII SAPASPLLGF LVDKTGRNVM WVMLAVITTL LSHMMLAFTF WNPWIAMSLL
     GVSYSLLACA LWPMVAFVVP EHQLGTAYGF MQSIQNLGLA VMSIAAGSIL DVRGYLFLEV
     FFIACLCMAL LAVVLLYLFD YYKEGGLNLS AATRSKRAKP E