ID   MFSD1_PONAB             Reviewed;         465 AA.
AC   Q5R8G5;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Major facilitator superfamily domain-containing protein 1;
GN   Name=MFSD1;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Lysosomal transporter which is essential for liver
CC       homeostasis. Required to maintain stability and lysosomal localization
CC       of GLMP. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SUBUNIT: Homodimer. Interacts with lysosomal protein GLMP (via lumenal
CC       domain); the interaction starts while both proteins are still in the
CC       endoplasmic reticulum and is required for stability and lysosomal
CC       localization of MFSD1. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q9DC37};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- DOMAIN: The dileucine internalization motif is required for lysosomal
CC       localization. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- PTM: Not N-glycosylated. {ECO:0000250|UniProtKB:Q9DC37}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; CR859787; CAH91945.1; -; mRNA.
DR   RefSeq; NP_001127485.1; NM_001134013.1.
DR   AlphaFoldDB; Q5R8G5; -.
DR   SMR; Q5R8G5; -.
DR   STRING; 9601.ENSPPYP00000015931; -.
DR   GeneID; 100174559; -.
DR   KEGG; pon:100174559; -.
DR   CTD; 64747; -.
DR   eggNOG; KOG4686; Eukaryota.
DR   InParanoid; Q5R8G5; -.
DR   OrthoDB; 941048at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0061462; P:protein localization to lysosome; ISS:UniProtKB.
DR   GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Lysosome; Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..465
FT                   /note="Major facilitator superfamily domain-containing
FT                   protein 1"
FT                   /id="PRO_0000273384"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        361..381
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..412
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        418..438
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           11..12
FT                   /note="Dileucine internalization motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q9DC37"
SQ   SEQUENCE   465 AA;  51187 MW;  BC35343A12630FD5 CRC64;
     MEEEDEEARA LLAGGPDEAD RGAPAAPGAL PALCDPSRLV HRLLVLLLMC FLGFGSYFCY
     DNPAALQTQV KRDMQVNTTK FMLLYAWYSW SNVVLCFFGG FLIDRVFGIR WGTIIFSCFV
     CIGQVVFALG GIFNAFWLME FGRFVFGIGG ESLAVAQNTY AVSWFKGKEL NLVFGLQLSM
     ARIGSTVNMN LMGWLYSKIE ALLGSAGHTT LGITLMIGGV TCILSLICAL ALAYLDQRAE
     RILHKEQGKT GEVIKLTDVK DFSLPLWLIF IICVCYYVAV FPFIGLGKVF FTEKFGFSSQ
     AASAINSVVY VISAPMSPVF GLLVDKTGKN IIWVLCAVAA TLVSHMMLAF TMWNPWIAMC
     LLGLSYSLLA CALWPMVAFV VPEHQLGTAH GFMQSIQNLG LAIISIIAGM ILDSRGYLFL
     EVFFIACVSL SLLSVVLLYL VNRAQGGNLN YSARQREEIK FSHTE