MFSD6_MOUSE
ID MFSD6_MOUSE Reviewed; 775 AA.
AC Q8CBH5; Q8C8K4; Q8VIG4;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Major facilitator superfamily domain-containing protein 6;
DE AltName: Full=Macrophage MHC class I receptor 2;
GN Name=Mfsd6; Synonyms=Mmr2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 14-775 (ISOFORM 1), AND FUNCTION.
RC STRAIN=C57BL/6J; TISSUE=Macrophage;
RX PubMed=17010536; DOI=10.1016/j.gene.2006.07.004;
RA Tashiro-Yamaji J., Kubota T., Yoshida R.;
RT "Macrophage MHC receptor 2: a novel receptor on allograft (H-2D(d)K(d))-
RT induced macrophage (H-2D(b)K(b)) recognizing an MHC class I molecule, H-
RT 2K(d), in mice.";
RL Gene 384:1-8(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 183-775 (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: MHC class I receptor. Binds only to H-2 class I
CC histocompatibility antigen, K-D alpha chain (H-2K(D)).
CC {ECO:0000269|PubMed:17010536}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CBH5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CBH5-2; Sequence=VSP_031836;
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. MFSD6 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH17534.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC32900.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC32900.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAF36946.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK035995; BAC29272.1; -; mRNA.
DR EMBL; AK046866; BAC32900.1; ALT_SEQ; mRNA.
DR EMBL; AB247936; BAF36946.1; ALT_INIT; mRNA.
DR EMBL; BC017534; AAH17534.1; ALT_INIT; mRNA.
DR CCDS; CCDS14946.1; -. [Q8CBH5-1]
DR RefSeq; NP_835182.1; NM_178081.4. [Q8CBH5-1]
DR AlphaFoldDB; Q8CBH5; -.
DR STRING; 10090.ENSMUSP00000122881; -.
DR TCDB; 2.A.1.65.6; the major facilitator superfamily (mfs).
DR iPTMnet; Q8CBH5; -.
DR SwissPalm; Q8CBH5; -.
DR EPD; Q8CBH5; -.
DR jPOST; Q8CBH5; -.
DR MaxQB; Q8CBH5; -.
DR PaxDb; Q8CBH5; -.
DR PeptideAtlas; Q8CBH5; -.
DR PRIDE; Q8CBH5; -.
DR ProteomicsDB; 293466; -. [Q8CBH5-1]
DR ProteomicsDB; 293467; -. [Q8CBH5-2]
DR Antibodypedia; 19836; 108 antibodies from 22 providers.
DR Ensembl; ENSMUST00000087701; ENSMUSP00000084991; ENSMUSG00000041439. [Q8CBH5-1]
DR GeneID; 98682; -.
DR KEGG; mmu:98682; -.
DR UCSC; uc007aym.1; mouse. [Q8CBH5-1]
DR CTD; 54842; -.
DR MGI; MGI:1922925; Mfsd6.
DR VEuPathDB; HostDB:ENSMUSG00000041439; -.
DR eggNOG; KOG3762; Eukaryota.
DR GeneTree; ENSGT00530000063599; -.
DR InParanoid; Q8CBH5; -.
DR BioGRID-ORCS; 98682; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Mfsd6; mouse.
DR PRO; PR:Q8CBH5; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8CBH5; protein.
DR Bgee; ENSMUSG00000041439; Expressed in motor neuron and 236 other tissues.
DR ExpressionAtlas; Q8CBH5; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0042288; F:MHC class I protein binding; IPI:MGI.
DR GO; GO:0032393; F:MHC class I receptor activity; IPI:MGI.
DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0042590; P:antigen processing and presentation of exogenous peptide antigen via MHC class I; IDA:MGI.
DR Gene3D; 1.20.1250.20; -; 3.
DR InterPro; IPR024989; MFS_assoc_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF12832; MFS_1_like; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Adaptive immunity; Alternative splicing; Immunity; Membrane;
KW Phosphoprotein; Receptor; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..775
FT /note="Major facilitator superfamily domain-containing
FT protein 6"
FT /id="PRO_0000321941"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 510..530
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 547..567
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 582..602
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 608..628
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 28..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 11
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT VAR_SEQ 728..775
FT /note="VTLCWASVASAPLLPPCSSKHMGNRKTGMLAKDISGLRSLCHSVYQVA ->
FT GTSENREASPAGGAQRAPRETHSASPRNQPSPDTAASQTQSSPAHPS (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031836"
SQ SEQUENCE 775 AA; 86076 MW; AD85C3E94AF135E9 CRC64;
MAADDKVAIL TDDEEEQKRK YVLADPFNGI CREPEPPSNE TPSSTETSAI PEEEIDWIEK
HCVKVNNDLL ISKVFYFFFY SAYGSLYPLL PVYYKQLGMS PSQSGLLVGI RYFIEFCSAP
FWGVVADRFR KGKIVLLFSL LCWVLFNLGI GFVKPATLRC LPKIPPTAHP TNVSHPVTVL
PMNSSTVAFF STPPKLLQKR DVQLSETEPN ISDIDLVSTA LTLTSEPTRR PQTEAITHPV
TGLILNTSTV TLPPTGNVTR ETTIAVVTTT KSLPSDQVTL VYDQQEVEAI FLIILVVVII
GEFFSASSVT IVDTVTLQYL GKHRDRYGLQ RMWGSLGWGL AMLSVGIGID YTHIDVLIDG
KGCKPPEYRN YQIVFIVFGV LMTMALIVAT QFRFRYNHFN NSDGKGKEVE IPQVERDNST
ESSEETPTAA THSQAFNFWD LIKLLCSVQY GSVLFVAWFM GFGYGFVFTF LYWHLEDLNG
TTTLFGVCSV LSHVSELTAY FFSHKLIELI GHIRVLYIGL ACNTARYIYI SYLENAWTVL
PMEVLQGVTH AAIWAACISY LSAAVPPELR TSAQGILQGL HLGLGRGCGA MIGGVLVNYF
GAAATFRGIG MACLVILLLF ALIQWLAVPD EEEDKTMLAE RIPVPSSPVP IATIDLVQQQ
TEDVMPRVEA RLPPKKTKHQ EEQEDVNKPA WGVSSSPWVT FVYALYQVKE LIQLTRESRA
SEIQPLQVTL CWASVASAPL LPPCSSKHMG NRKTGMLAKD ISGLRSLCHS VYQVA
