ID   MFSD8_DANRE             Reviewed;         504 AA.
AC   Q0VA82;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Major facilitator superfamily domain-containing protein 8;
GN   Name=mfsd8; ORFNames=zgc:136865;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May be a carrier that transport small solutes by using
CC       chemiosmotic ion gradients. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}. Note=Sorting to lysosomes involves
CC       tyrosine- and/or dileucine-based motifs. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC121197; AAI21198.1; -; mRNA.
DR   RefSeq; NP_001038513.1; NM_001045048.1.
DR   AlphaFoldDB; Q0VA82; -.
DR   SMR; Q0VA82; -.
DR   STRING; 7955.ENSDARP00000050073; -.
DR   PaxDb; Q0VA82; -.
DR   PRIDE; Q0VA82; -.
DR   Ensembl; ENSDART00000168935; ENSDARP00000140706; ENSDARG00000103235.
DR   Ensembl; ENSDART00000184266; ENSDARP00000152621; ENSDARG00000115968.
DR   GeneID; 564342; -.
DR   KEGG; dre:564342; -.
DR   CTD; 256471; -.
DR   ZFIN; ZDB-GENE-060818-29; mfsd8.
DR   eggNOG; KOG2325; Eukaryota.
DR   GeneTree; ENSGT00530000063854; -.
DR   HOGENOM; CLU_027024_2_0_1; -.
DR   InParanoid; Q0VA82; -.
DR   OMA; AWSNDEA; -.
DR   OrthoDB; 685352at2759; -.
DR   PhylomeDB; Q0VA82; -.
DR   PRO; PR:Q0VA82; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 12.
DR   Bgee; ENSDARG00000103235; Expressed in mature ovarian follicle and 20 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Lysosome; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..504
FT                   /note="Major facilitator superfamily domain-containing
FT                   protein 8"
FT                   /id="PRO_0000311234"
FT   TOPO_DOM        1..32
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        54..66
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        88..97
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        119..123
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        145..169
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        191..203
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        204..224
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        225..258
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        259..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        280..302
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        303..323
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        324..330
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        352..468
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        469..489
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        490..504
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOTIF           12..13
FT                   /note="Dileucine internalization motif"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        364
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   504 AA;  55486 MW;  BEBDBE6F3E94AFC0 CRC64;
     MPLEESGENT PLLRGEIISD GDTDRSRWRS IRVMYFTMFL SSVGFTIVIT SIWPYLKKID
     ESADASFLGW VVAAYSLGQM VASPFFGLWS NHRPRREPLV CSIFINVSAN IYYSYVYLPP
     SHNQIHMLLA RTFVGIGAGN VAVVRSYVAG ATSLKERTSA MANMSACQAL GFILGPALQA
     VLSFIGETGV SVDVIKLQVN MYTAPALLAA CFGVINILLV ILVLREHSVD DHGNRIRPIN
     YTPEERVDVP PEVEGDIDHI AVFTSNVLFF IILFIFAVFE TISTPLSMDM FAWTRKEAVL
     YNGIILAAIG FESILVFLVV KVVSARVGDR PLLLGGLILI FVGFFMLLPW GNQYPKIQWT
     DINNNTIPPI QLAPTPASNS SLEPIGCPSE QTWCLFTPVI HLAQYITSDI LIGVGYPTCN
     VMSYTLYSKI LGPKPQGVYM GWLTASGSGA RTLGPVFVSH FYTILGPRWA FSAICGIVLA
     AIVMLSAMYK RLVAFSIRHG RIAE