MFSEP_MYCS2
ID MFSEP_MYCS2 Reviewed; 429 AA.
AC A0QYL8;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=MFS-type efflux pump MSMEG_3705 {ECO:0000305};
GN OrderedLocusNames=MSMEG_3705 {ECO:0000312|EMBL:ABK72667.1},
GN MSMEI_3617 {ECO:0000312|EMBL:AFP40080.1};
GN ORFNames=LJ00_18410 {ECO:0000312|EMBL:AIU08832.1};
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=25657281; DOI=10.1128/genomea.01520-14;
RA Mohan A., Padiadpu J., Baloni P., Chandra N.;
RT "Complete genome sequences of a Mycobacterium smegmatis laboratory strain
RT (MC2 155) and isoniazid-resistant (4XR1/R2) mutant strains.";
RL Genome Announc. 3:E01520-E01520(2015).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=25697715; DOI=10.1007/s00284-015-0783-0;
RA Zhang Z., Wang R., Xie J.;
RT "Mycobacterium smegmatis MSMEG_3705 encodes a selective major facilitator
RT superfamily efflux pump with multiple roles.";
RL Curr. Microbiol. 70:801-809(2015).
CC -!- FUNCTION: Probably plays a role in bacterial growth and resistance to
CC antibiotics. {ECO:0000269|PubMed:25697715}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant is more sensitive to capreomycin,
CC more resistant to rifampicin, and accumulates more ethidium bromide in
CC the cell than the wild type. {ECO:0000269|PubMed:25697715}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; CP000480; ABK72667.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP40080.1; -; Genomic_DNA.
DR EMBL; CP009494; AIU08832.1; -; Genomic_DNA.
DR RefSeq; WP_011729275.1; NZ_SIJM01000041.1.
DR RefSeq; YP_888006.1; NC_008596.1.
DR AlphaFoldDB; A0QYL8; -.
DR SMR; A0QYL8; -.
DR STRING; 246196.MSMEI_3617; -.
DR TCDB; 2.A.1.49.10; the major facilitator superfamily (mfs).
DR EnsemblBacteria; ABK72667; ABK72667; MSMEG_3705.
DR EnsemblBacteria; AFP40080; AFP40080; MSMEI_3617.
DR GeneID; 66735084; -.
DR KEGG; msb:LJ00_18410; -.
DR KEGG; msg:MSMEI_3617; -.
DR KEGG; msm:MSMEG_3705; -.
DR PATRIC; fig|246196.19.peg.3652; -.
DR eggNOG; COG2814; Bacteria.
DR HOGENOM; CLU_001265_5_12_11; -.
DR OMA; WYVVVIC; -.
DR OrthoDB; 1345431at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR CDD; cd17328; MFS_spinster_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR044770; MFS_spinster-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR23505; PTHR23505; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00457; NA_SOLUT_SYMP_2; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..429
FT /note="MFS-type efflux pump MSMEG_3705"
FT /id="PRO_0000432862"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 429 AA; 44969 MW; CA9AB3C839FA6A80 CRC64;
MSAPQAAIDT DHADRHGPRR AWAAVGVLAL VGTLNYVDRF LPSVLAEPIK HDLELSDTAI
GVINGFGFLI VYAVMGIAVA RVADRGAFGA VVAGCLTLWG TMTMLGGAVQ SGFQLALTRV
GVAIGEAGST PAAHAYVARN FVPQRRSAPL AVITIAIPLA STASLLGGGL LAQSLGWRTA
FVIMGAVSVV LAPLVLLVVG VRQSLPAAPA VVDKTAGGWW NLLRKPSFLI VVAGTAFISA
AGYSLTTFSP AFLMRTRGMS LGEVGVEYGL ATGAIGVLGL LIVGRLADRL AERDPRWLLW
IVVTLTLVLL PASVLAFVVE DRMLCVLFLA LSYAIGTSYL APSIAAIQRL VLPEQRATAS
AMFLFFNAVF GSVGPFVVGM LSDSLTDDLG AQALGRALLL LVAAMQLVGA ICYWLASARY
RRDIIEEAR
