MFS_LEPMJ
ID MFS_LEPMJ Reviewed; 628 AA.
AC E5AE35;
DT 10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 1.
DT 25-MAY-2022, entry version 41.
DE RecName: Full=Phomenoic acid biosynthesis cluster MFS-type transporter {ECO:0000303|PubMed:23396262};
GN Name=MFS {ECO:0000303|PubMed:23396262}; ORFNames=LEMA_P002610.1;
OS Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8)
OS (Blackleg fungus) (Phoma lingam).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Leptosphaeriaceae;
OC Leptosphaeria; Leptosphaeria maculans species complex.
OX NCBI_TaxID=985895;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8;
RX PubMed=21326234; DOI=10.1038/ncomms1189;
RA Rouxel T., Grandaubert J., Hane J.K., Hoede C., van de Wouw A.P.,
RA Couloux A., Dominguez V., Anthouard V., Bally P., Bourras S.,
RA Cozijnsen A.J., Ciuffetti L.M., Degrave A., Dilmaghani A., Duret L.,
RA Fudal I., Goodwin S.B., Gout L., Glaser N., Linglin J., Kema G.H.J.,
RA Lapalu N., Lawrence C.B., May K., Meyer M., Ollivier B., Poulain J.,
RA Schoch C.L., Simon A., Spatafora J.W., Stachowiak A., Turgeon B.G.,
RA Tyler B.M., Vincent D., Weissenbach J., Amselem J., Quesneville H.,
RA Oliver R.P., Wincker P., Balesdent M.-H., Howlett B.J.;
RT "Effector diversification within compartments of the Leptosphaeria maculans
RT genome affected by Repeat-Induced Point mutations.";
RL Nat. Commun. 2:202-202(2011).
RN [2]
RP IDENTIFICATION, FUNCTION, AND INDUCTION.
RX PubMed=23396262; DOI=10.1016/j.fgb.2013.01.008;
RA Elliott C.E., Callahan D.L., Schwenk D., Nett M., Hoffmeister D.,
RA Howlett B.J.;
RT "A gene cluster responsible for biosynthesis of phomenoic acid in the plant
RT pathogenic fungus, Leptosphaeria maculans.";
RL Fungal Genet. Biol. 53:50-58(2013).
CC -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates
CC the biosynthesis of phomenoic acid, a long chain aliphatic carboxylic
CC acid that does not appear to be essential for pathogenicity but may
CC play a role in allowing to outcompete other fungi in the environmental
CC niche via its antifungal properties (PubMed:23396262). Is probably
CC involved in the efflux of phomenoic acid (Probable).
CC {ECO:0000269|PubMed:23396262, ECO:0000305|PubMed:23396262}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expression is positively regulated by the phomenoic acid
CC biosynthesis cluster-specific transcription regulator C6TF.
CC {ECO:0000269|PubMed:23396262}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
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DR EMBL; FP929139; CBY01474.1; -; Genomic_DNA.
DR RefSeq; XP_003844953.1; XM_003844905.1.
DR AlphaFoldDB; E5AE35; -.
DR SMR; E5AE35; -.
DR EnsemblFungi; CBY01474; CBY01474; LEMA_P002610.1.
DR GeneID; 13290705; -.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_000960_22_1_1; -.
DR InParanoid; E5AE35; -.
DR OMA; GGNGMYL; -.
DR OrthoDB; 503593at2759; -.
DR Proteomes; UP000002668; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..628
FT /note="Phomenoic acid biosynthesis cluster MFS-type
FT transporter"
FT /id="PRO_0000446537"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 595..615
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 628 AA; 67838 MW; 9710B16036B48240 CRC64;
MSTLCAFPVP YSTIEVLNIV LPYRTYSARL LYLHAPLGNH LPFCHRSYNV LHLRQDCDSH
MYSAGNDVRL SQEAVTMSDQ RSHDTTADQS ARDASSLDTR KIHGFPWFLL VISVLSSIFL
YALDNTIVAD IIPAIANDFS SINDLGWLSV GFVIGGVAVI MPLGKLYGIL NTKWLYITSV
VLFMAASAVC GAAPDMNAEI VGRVFAGAGG IGMYIGTMIL LSINTSDQER PAYLSLVGLV
WGIGTVLGPV IGGSFEKVDW RWAFYLNLVI GALLFPVWFF LLPSFHPAKH LSLNKRLSQF
DFVGAILSIG AFVTTIMPIN FGGTTYEWNS GTIIALFVVS GVLWIAFAVQ QSLTLFTTPA
DRMFPVQFLK NKEAMLLFIC AAAINSAVFV PIYFIPIYFQ FSRGDGALDS AIRLLPLIFL
LCATILVNGK LMSRFGYYMP WYLVGGVFCL IANVFLCELH PVAAVLVRYT DYKTALLDAT
TSQSYIYGFE ALLGLGAGGS VQAGYAVIQT VVPATDLGYA VSFIMIAQIG GIALGLACAS
AVFINGATNS LRVLLPLLSD SELQSAISGT GGRFLETLDD NTRVQVIDAV VNNLAKAFIV
AYAGAAVSLV ASLGFSRKRV FLPTAAAA
