ID   MFTA_MYCTO              Reviewed;          29 AA.
AC   P9WJ80; P0DJC2; Q8VKG2;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2021, sequence version 2.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Mycofactocin precursor peptide {ECO:0000305};
GN   Name=mftA; OrderedLocusNames=MT0719.1;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Precursor peptide that leads to mycofactocin (MFT) after
CC       extensive post-translational modifications by enzymes encoded by
CC       adjacent genes. Mycofactocin acts as a redox cofactor of nicotinamide-
CC       dependent oxidoreductases encoded in the same locus.
CC       {ECO:0000250|UniProtKB:P0DUE9}.
CC   -!- PTM: The post-translational modifications that lead to mycofactocin
CC       involve oxidative decarboxylation of the C-terminal tyrosine residue
CC       catalyzed by MftC, introduction of a tyramine-valine cross-link,
CC       removal of the modified C-terminal dipeptide by MftE. The released
CC       dipeptide then undergoes oxidative deamination by MftD, glycosylation
CC       by MftF and methylation by an unknown enzyme.
CC       {ECO:0000250|UniProtKB:P0DUE9}.
CC   -!- MISCELLANEOUS: Mycofactocin is classified as a ribosomally synthesized
CC       and post-translationally modified peptide (RiPP). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the mycofactocin precursor peptide family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK44947.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK44947.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003403483.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WJ80; -.
DR   EnsemblBacteria; AAK44947; AAK44947; MT0719.1.
DR   GeneID; 45428029; -.
DR   KEGG; mtc:MT0719.1; -.
DR   PATRIC; fig|83331.31.peg.768; -.
DR   HOGENOM; CLU_2899408_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   InterPro; IPR023988; MftA.
DR   TIGRFAMs; TIGR03969; mycofactocin; 1.
PE   3: Inferred from homology;
FT   CHAIN           1..29
FT                   /note="Mycofactocin precursor peptide"
FT                   /id="PRO_0000427876"
FT   SITE            27..28
FT                   /note="Cleavage; by MftE"
FT                   /evidence="ECO:0000250|UniProtKB:P0DUE9"
SQ   SEQUENCE   29 AA;  3259 MW;  770600C0675FC646 CRC64;
     MDYETDTDTE LVTETLVEEV SIDGMCGVY