MFTA_MYCTU
ID MFTA_MYCTU Reviewed; 29 AA.
AC P9WJ81; P0DJC2; Q8VKG2;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2021, sequence version 2.
DT 25-MAY-2022, entry version 26.
DE RecName: Full=Mycofactocin precursor peptide {ECO:0000305};
GN Name=mftA {ECO:0000303|PubMed:21223593}; OrderedLocusNames=Rv0691A;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION, AND POSSIBLE FUNCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21223593; DOI=10.1186/1471-2164-12-21;
RA Haft D.H.;
RT "Bioinformatic evidence for a widely distributed, ribosomally produced
RT electron carrier precursor, its maturation proteins, and its
RT nicotinoprotein redox partners.";
RL BMC Genomics 12:21-21(2011).
CC -!- FUNCTION: Precursor peptide that leads to mycofactocin (MFT) after
CC extensive post-translational modifications by enzymes encoded by
CC adjacent genes. Mycofactocin acts as a redox cofactor of nicotinamide-
CC dependent oxidoreductases encoded in the same locus.
CC {ECO:0000250|UniProtKB:P0DUE9}.
CC -!- PTM: The post-translational modifications that lead to mycofactocin
CC involve oxidative decarboxylation of the C-terminal tyrosine residue
CC catalyzed by MftC, introduction of a tyramine-valine cross-link,
CC removal of the modified C-terminal dipeptide by MftE. The released
CC dipeptide then undergoes oxidative deamination by MftD, glycosylation
CC by MftF and methylation by an unknown enzyme.
CC {ECO:0000250|UniProtKB:P0DUE9}.
CC -!- MISCELLANEOUS: Mycofactocin is classified as a ribosomally synthesized
CC and post-translationally modified peptide (RiPP). {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the mycofactocin precursor peptide family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCP43435.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL123456; CCP43435.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_003403483.1; NZ_NVQJ01000007.1.
DR RefSeq; YP_007409348.1; NC_000962.3.
DR AlphaFoldDB; P9WJ81; -.
DR STRING; 83332.Rv0691A; -.
DR GeneID; 14515851; -.
DR GeneID; 45428029; -.
DR KEGG; mtu:Rv0691A; -.
DR PATRIC; fig|83332.111.peg.767; -.
DR TubercuList; Rv0691A; -.
DR eggNOG; ENOG5031WMY; Bacteria.
DR Proteomes; UP000001584; Chromosome.
DR InterPro; IPR023988; MftA.
DR TIGRFAMs; TIGR03969; mycofactocin; 1.
PE 3: Inferred from homology;
KW Reference proteome.
FT CHAIN 1..29
FT /note="Mycofactocin precursor peptide"
FT /id="PRO_0000415282"
FT SITE 27..28
FT /note="Cleavage; by MftE"
FT /evidence="ECO:0000250|UniProtKB:P0DUE9"
SQ SEQUENCE 29 AA; 3259 MW; 770600C0675FC646 CRC64;
MDYETDTDTE LVTETLVEEV SIDGMCGVY