ID   MFTC_MACFA              Reviewed;         315 AA.
AC   Q95J75;
DT   22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Mitochondrial folate transporter/carrier;
DE   AltName: Full=Solute carrier family 25 member 32;
GN   Name=SLC25A32; Synonyms=MFTC; ORFNames=QmoA-10785, QtrA-13024;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Medulla oblongata, and Temporal cortex;
RA   Osada N., Hida M., Kusuda J., Tanuma R., Iseki K., Hirai M., Terao K.,
RA   Suzuki Y., Sugano S., Hashimoto K.;
RT   "Isolation of full-length cDNA clones from macaque brain cDNA libraries.";
RL   Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transports folate across the inner membranes of mitochondria
CC       (By similarity). Can also transport FAD across the mitochondrial inner
CC       membrane (By similarity). {ECO:0000250|UniProtKB:Q9H2D1}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q6IZB5}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
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DR   EMBL; AB060884; BAB46890.1; -; mRNA.
DR   EMBL; AB062992; BAB60754.1; -; mRNA.
DR   RefSeq; XP_005563951.1; XM_005563894.2.
DR   AlphaFoldDB; Q95J75; -.
DR   SMR; Q95J75; -.
DR   STRING; 9541.XP_005563951.1; -.
DR   Ensembl; ENSMFAT00000018118; ENSMFAP00000043828; ENSMFAG00000039281.
DR   GeneID; 102144981; -.
DR   KEGG; mcf:102144981; -.
DR   CTD; 81034; -.
DR   VEuPathDB; HostDB:ENSMFAG00000039281; -.
DR   eggNOG; KOG0764; Eukaryota.
DR   GeneTree; ENSGT00920000149145; -.
DR   OMA; GLYFLWY; -.
DR   OrthoDB; 1080385at2759; -.
DR   Proteomes; UP000233100; Chromosome 8.
DR   Bgee; ENSMFAG00000039281; Expressed in liver and 13 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0008517; F:folic acid transmembrane transporter activity; IEA:Ensembl.
DR   GO; GO:1904947; P:folate import into mitochondrion; ISS:UniProtKB.
DR   GO; GO:1990548; P:mitochondrial FAD transmembrane transport; ISS:UniProtKB.
DR   GO; GO:0006839; P:mitochondrial transport; IEA:InterPro.
DR   Gene3D; 1.50.40.10; -; 1.
DR   InterPro; IPR002030; Mit_uncoupling_UCP-like.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   InterPro; IPR044712; SLC25A32-like.
DR   PANTHER; PTHR45683; PTHR45683; 1.
DR   Pfam; PF00153; Mito_carr; 3.
DR   PRINTS; PR00784; MTUNCOUPLING.
DR   SUPFAM; SSF103506; SSF103506; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..315
FT                   /note="Mitochondrial folate transporter/carrier"
FT                   /id="PRO_0000090642"
FT   TRANSMEM        26..43
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..106
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..203
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        227..243
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        281..300
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          20..109
FT                   /note="Solcar 1"
FT   REPEAT          118..209
FT                   /note="Solcar 2"
FT   REPEAT          222..306
FT                   /note="Solcar 3"
FT   SITE            91
FT                   /note="Important for substrate recognition"
FT                   /evidence="ECO:0000250|UniProtKB:Q6IZB5"
FT   SITE            142
FT                   /note="Important for substrate recognition"
FT                   /evidence="ECO:0000250|UniProtKB:Q6IZB5"
FT   SITE            249
FT                   /note="Important for substrate recognition"
FT                   /evidence="ECO:0000250|UniProtKB:Q6IZB5"
SQ   SEQUENCE   315 AA;  35416 MW;  00AE5B0A774590F8 CRC64;
     MTGQGHSASG SSAWSTVFRH VRYENLVAGV SGGVLSNLAL HPLDLVKIRF AVSDGLELRP
     KYNGILHCLT TIWKLDGLRG LYQGVTPNVW GAGLSWGLYF FFYNAIKSYK TEGRAERLEA
     TEYLVSAAEA GAMTLCITNP LWVTKTRLML QYDAVINSPH RQYKGMFDTL VKIYKYEGVR
     GLYKGFVPGL FGTSHGALQF MAYELLKLKY NQHINRLPEA QLSTVEYISV AALSKIFAVA
     ATYPYQVVRA RLQDQHMFYS GVIDVITKTW RKEGIGGFYK GIAPNLIRVT PACCITFVVY
     ENVSHFLLDL REKRK