ID   MFTF_MYCTU              Reviewed;         470 AA.
AC   P9WMX1; L0T4G0; P95042; Q7D9E7;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Pre-mycofactocin glycosyltransferase;
DE            EC=2.4.1.- {ECO:0000250|UniProtKB:A0QSC1};
GN   Name=mftF {ECO:0000303|PubMed:21223593}; OrderedLocusNames=Rv0696;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21223593; DOI=10.1186/1471-2164-12-21;
RA   Haft D.H.;
RT   "Bioinformatic evidence for a widely distributed, ribosomally produced
RT   electron carrier precursor, its maturation proteins, and its
RT   nicotinoprotein redox partners.";
RL   BMC Genomics 12:21-21(2011).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Involved in the biosynthesis of the enzyme cofactor
CC       mycofactocin (MFT). Acts as a glycosyltransferase that catalyzes the
CC       oligoglycosylation of pre-mycofactocin (PMFT), adding up to nine beta-
CC       1,4-linked glucose residues. {ECO:0000250|UniProtKB:A0QSC1}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43440.1; -; Genomic_DNA.
DR   PIR; C70641; C70641.
DR   RefSeq; NP_215210.1; NC_000962.3.
DR   RefSeq; WP_003403501.1; NZ_NVQJ01000007.1.
DR   AlphaFoldDB; P9WMX1; -.
DR   SMR; P9WMX1; -.
DR   STRING; 83332.Rv0696; -.
DR   PaxDb; P9WMX1; -.
DR   DNASU; 888307; -.
DR   GeneID; 888307; -.
DR   KEGG; mtu:Rv0696; -.
DR   TubercuList; Rv0696; -.
DR   eggNOG; COG1216; Bacteria.
DR   OMA; HVAHDHR; -.
DR   PhylomeDB; P9WMX1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR023981; MftF.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR43646:SF6; PTHR43646:SF6; 1.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
DR   TIGRFAMs; TIGR03965; mycofact_glyco; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Glycosyltransferase; Membrane; Reference proteome;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..470
FT                   /note="Pre-mycofactocin glycosyltransferase"
FT                   /id="PRO_0000415278"
FT   TRANSMEM        315..335
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   470 AA;  50667 MW;  D2D6AC3F43D77414 CRC64;
     MTATRLPDGF AVQVDRRVRV LGDGSALLGG SPTRLLRLAP AARGLLCDGR LKVRDEVSAE
     LARILLDATV AHPRPPSGPS HRDVTVVIPV RNNASGLRRL VTSLRGLRVI VVDDGSACPV
     ESDDFVGAHC DIEVLHHPHS KGPAAARNTG LAACTTDFVA FLDSDVTPRR GWLESLLGHF
     CDPTVALVAP RIVSLVEGEN PVARYEALHS SLDLGQREAP VLPHSTVSYV PSAAIVCRSS
     AIRDVGGFDE TMHSGEDVDL CWRLIEAGAR LRYEPIALVA HDHRTQLRDW IARKAFYGGS
     AAPLAVRHPD KTAPLVISGG ALMAWILMSI GTGLGRLASL VIAVLTGRRI ARAMRCAETS
     FLDVLAVATR GLWAAALQLA SAICRHYWPL ALLAAILSRR CRRVVLIAAV VDGVVDWLRR
     REGADDDAEP IGPLTYLVLK RVDDLAYGAG LWYGVVRERN IGALKPQIRT