MFT_ARATH
ID MFT_ARATH Reviewed; 173 AA.
AC Q9XFK7;
DT 05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Protein MOTHER of FT and TFL1;
GN Name=MFT; OrderedLocusNames=At1g18100;
GN ORFNames=E12A11, T10F20.11, T10F20.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Kardailsky I., Weigel D.;
RT "A new Arabidopsis member of the TFL1/FT gene family.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION.
RC STRAIN=cv. Columbia;
RX PubMed=10583960; DOI=10.1126/science.286.5446.1960;
RA Kobayashi Y., Kaya H., Goto K., Iwabuchi M., Araki T.;
RT "A pair of related genes with antagonistic roles in mediating flowering
RT signals.";
RL Science 286:1960-1962(1999).
RN [6]
RP FUNCTION, INDUCTION BY ABSCISIC ACID, AND DISRUPTION PHENOTYPE.
RX PubMed=20551347; DOI=10.1105/tpc.109.073072;
RA Xi W., Liu C., Hou X., Yu H.;
RT "MOTHER OF FT AND TFL1 regulates seed germination through a negative
RT feedback loop modulating ABA signaling in Arabidopsis.";
RL Plant Cell 22:1733-1748(2010).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=20935478; DOI=10.4161/psb.5.10.13161;
RA Xi W., Yu H.;
RT "MOTHER OF FT AND TFL1 regulates seed germination and fertility relevant to
RT the brassinosteroid signaling pathway.";
RL Plant Signal. Behav. 5:1315-1317(2010).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: May form complexes with phosphorylated ligands by interfering
CC with kinases and their effectors (By similarity). Regulates seed
CC germination via the abscisic acid (ABA) and gibberellic acid
CC (GA)signaling pathways. During seed germination, MFT expression is
CC directly repressed by ABI3 or promoted by ABI5 in the ABA signaling
CC pathway. Involved in a negative feedback regulation of ABA signaling.
CC Promotes embryo growth by direct repression of ABI5. In the GA
CC signaling pathway, MFT expression is promoted by the DELLA protein RGL2
CC during seed germination (PubMed:20551347). May regulate seed
CC germination and fertility through the brassinosteroid (BR) signaling
CC pathway (PubMed:20935478). {ECO:0000250|UniProtKB:P30086,
CC ECO:0000269|PubMed:20551347, ECO:0000269|PubMed:20935478}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in gametophytes and developing seeds.
CC {ECO:0000269|PubMed:20935478}.
CC -!- INDUCTION: By abscisic acid. {ECO:0000269|PubMed:20551347}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant seeds show hypersensitivity to ABA during seed
CC germination. {ECO:0000269|PubMed:20551347}.
CC -!- SIMILARITY: Belongs to the phosphatidylethanolamine-binding protein
CC family. {ECO:0000305}.
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DR EMBL; AF147721; AAD37380.1; -; mRNA.
DR EMBL; AC034107; AAF97827.1; -; Genomic_DNA.
DR EMBL; AC069551; AAF78390.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29676.1; -; Genomic_DNA.
DR EMBL; AF332406; AAG48769.1; -; mRNA.
DR RefSeq; NP_173250.1; NM_101672.4.
DR AlphaFoldDB; Q9XFK7; -.
DR SMR; Q9XFK7; -.
DR STRING; 3702.AT1G18100.1; -.
DR iPTMnet; Q9XFK7; -.
DR PaxDb; Q9XFK7; -.
DR PRIDE; Q9XFK7; -.
DR ProteomicsDB; 238357; -.
DR EnsemblPlants; AT1G18100.1; AT1G18100.1; AT1G18100.
DR GeneID; 838390; -.
DR Gramene; AT1G18100.1; AT1G18100.1; AT1G18100.
DR KEGG; ath:AT1G18100; -.
DR Araport; AT1G18100; -.
DR TAIR; locus:2194100; AT1G18100.
DR eggNOG; KOG3346; Eukaryota.
DR HOGENOM; CLU_043994_6_1_1; -.
DR InParanoid; Q9XFK7; -.
DR OMA; AVVEYPC; -.
DR OrthoDB; 1557122at2759; -.
DR PhylomeDB; Q9XFK7; -.
DR PRO; PR:Q9XFK7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9XFK7; baseline and differential.
DR Genevisible; Q9XFK7; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010030; P:positive regulation of seed germination; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR CDD; cd00866; PEBP_euk; 1.
DR Gene3D; 3.90.280.10; -; 1.
DR InterPro; IPR008914; PEBP.
DR InterPro; IPR036610; PEBP-like_sf.
DR InterPro; IPR035810; PEBP_euk.
DR InterPro; IPR001858; Phosphatidylethanolamine-bd_CS.
DR PANTHER; PTHR11362; PTHR11362; 1.
DR Pfam; PF01161; PBP; 1.
DR SUPFAM; SSF49777; SSF49777; 1.
DR PROSITE; PS01220; PBP; 1.
PE 1: Evidence at protein level;
KW Abscisic acid signaling pathway; Acetylation; Cytoplasm;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..173
FT /note="Protein MOTHER of FT and TFL1"
FT /id="PRO_0000204765"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 173 AA; 19134 MW; 3D548E21AD959A54 CRC64;
MAASVDPLVV GRVIGDVLDM FIPTANMSVY FGPKHITNGC EIKPSTAVNP PKVNISGHSD
ELYTLVMTDP DAPSPSEPNM REWVHWIVVD IPGGTNPSRG KEILPYMEPR PPVGIHRYIL
VLFRQNSPVG LMVQQPPSRA NFSTRMFAGH FDLGLPVATV YFNAQKEPAS RRR
