ID   MGDE_SYNY3              Reviewed;         410 AA.
AC   P74167;
DT   16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Monoglucosyldiacylglycerol epimerase {ECO:0000303|PubMed:25197079};
DE            Short=GlcDG epimerase {ECO:0000303|PubMed:25197079};
DE            EC=5.1.3.34 {ECO:0000269|PubMed:25197079};
DE   Flags: Precursor;
GN   Name=MgdE {ECO:0000303|PubMed:25197079}; OrderedLocusNames=sll1376;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=25197079; DOI=10.1073/pnas.1403708111;
RA   Awai K., Ohta H., Sato N.;
RT   "Oxygenic photosynthesis without galactolipids.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:13571-13575(2014).
CC   -!- FUNCTION: Involved in the biosynthesis of galactolipids found in the
CC       photosynthetic membranes. Catalyzes the isomerization of
CC       monoglucosyldiacylglycerol (GlcDG) to yield
CC       monogalactosyldiacylglycerol (MGDG). {ECO:0000269|PubMed:25197079}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = a 1,2-
CC         diacyl-3-O-(beta-D-galactosyl)-sn-glycerol; Xref=Rhea:RHEA:46928,
CC         ChEBI:CHEBI:17615, ChEBI:CHEBI:75799; EC=5.1.3.34;
CC         Evidence={ECO:0000269|PubMed:25197079};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: The mutant lacks both
CC       monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol
CC       (DGDG), and accumulates GlcDG. It possesses intact thylakoid membranes
CC       and shows normal maximal photosynthetic activity, albeit with reduced
CC       utilization of light energy. {ECO:0000269|PubMed:25197079}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; BA000022; BAA18256.1; -; Genomic_DNA.
DR   PIR; S75695; S75695.
DR   AlphaFoldDB; P74167; -.
DR   SMR; P74167; -.
DR   STRING; 1148.1653341; -.
DR   PaxDb; P74167; -.
DR   EnsemblBacteria; BAA18256; BAA18256; BAA18256.
DR   KEGG; syn:sll1376; -.
DR   eggNOG; COG0300; Bacteria.
DR   eggNOG; COG3000; Bacteria.
DR   InParanoid; P74167; -.
DR   OMA; WRHHFDD; -.
DR   BioCyc; MetaCyc:MON-19314; -.
DR   BRENDA; 5.1.3.34; 382.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016857; F:racemase and epimerase activity, acting on carbohydrates and derivatives; IDA:UniProtKB.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Glycerol metabolism; Isomerase; Lipid metabolism;
KW   Membrane; Photosynthesis; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..14
FT                   /evidence="ECO:0000255"
FT   CHAIN           15..410
FT                   /note="Monoglucosyldiacylglycerol epimerase"
FT                   /id="PRO_0000435691"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        320
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z4W1"
SQ   SEQUENCE   410 AA;  45956 MW;  E74EAA3FC8987E33 CRC64;
     MAMAWLMGLG LALASVLWVE LVRDCYHALA HVWSPLYRLH GWHHRVFRSD LSVVSTEIYQ
     KAHWYNDVPE ALVMLAFGIW PPLLTWMWQG FSQWPLILAA SAGMVYTLGF LLSAIARGVG
     LPNADEITDL THRPGPFLTP PAPWMVNRTY HWRHHFDDPN AYFCGTLTLV DKMLGTALSL
     KGKKIAVTGA SGGFGQALLQ ELHQQGAKAI AITSCGQEVT VIGAEKTIPI RTEQWCIGQE
     DQLKDLLAEI DVLVINHGVN VHQRRDGQAI QEAYEVNTFS ALRLMELFLA TVKTNRHIAT
     KEIWVNTSEA EVNPAFSPLY ELSKRALGDV VTLKRLDSPC VIRKLILGPF KSKLNPVGIM
     SAAWVARQVV KGVKRDSRNI IVTINPITFI AFPVKEFFVS LYFRCFTKKS