ID   MGDG2_ORYSJ             Reviewed;         469 AA.
AC   Q6UTZ2; Q0J6L4; Q69LQ8;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 2.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Probable monogalactosyldiacylglycerol synthase 2, chloroplastic;
DE            Short=OsMGD2;
DE            EC=2.4.1.46;
DE   Flags: Precursor;
GN   Name=MGD2; OrderedLocusNames=Os08g0299400, LOC_Os08g20420;
GN   ORFNames=OsJ_025729, OSJNBa0063H21.123, OSJNBa0070J19.22, P0045D08.104;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15037733; DOI=10.1105/tpc.019273;
RA   Wu J., Yamagata H., Hayashi-Tsugane M., Hijishita S., Fujisawa M.,
RA   Shibata M., Itoh Y., Nakamura M., Sakaguchi M., Yoshihara R., Kobayashi H.,
RA   Itoh K., Karasawa W., Yamamoto M., Saji S., Katagiri S., Kanamori H.,
RA   Namiki N., Katayose Y., Matsumoto T., Sasaki T.;
RT   "Composition and structure of the centromeric region of rice chromosome
RT   8.";
RL   Plant Cell 16:967-976(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=14716315; DOI=10.1038/ng1289;
RA   Nagaki K., Cheng Z., Ouyang S., Talbert P.B., Kim M., Jones K.M.,
RA   Henikoff S., Buell C.R., Jiang J.;
RT   "Sequencing of a rice centromere uncovers active genes.";
RL   Nat. Genet. 36:138-145(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 62-469.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [6]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 62-469.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Involved in the synthesis of the major structural component
CC       of photosynthetic membranes. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + UDP-alpha-D-galactose = a 1,2-
CC         diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + H(+) + UDP;
CC         Xref=Rhea:RHEA:14945, ChEBI:CHEBI:15378, ChEBI:CHEBI:17615,
CC         ChEBI:CHEBI:17815, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.46;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAQ56578.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAD30710.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAD31731.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP004458; BAD30710.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP005819; BAD31731.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY360394; AAQ56578.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CM000145; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP008214; BAF23401.1; -; Genomic_DNA.
DR   EMBL; AP014964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK064148; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015649135.1; XM_015793649.1.
DR   AlphaFoldDB; Q6UTZ2; -.
DR   SMR; Q6UTZ2; -.
DR   STRING; 4530.OS08T0299400-01; -.
DR   CAZy; GT28; Glycosyltransferase Family 28.
DR   PaxDb; Q6UTZ2; -.
DR   PRIDE; Q6UTZ2; -.
DR   GeneID; 4345212; -.
DR   KEGG; osa:4345212; -.
DR   eggNOG; ENOG502QPXV; Eukaryota.
DR   HOGENOM; CLU_028367_3_1_1; -.
DR   InParanoid; Q6UTZ2; -.
DR   OrthoDB; 628561at2759; -.
DR   Proteomes; UP000000763; Chromosome 8.
DR   Proteomes; UP000007752; Chromosome 8.
DR   Proteomes; UP000059680; Chromosome 8.
DR   Genevisible; Q6UTZ2; OS.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046509; F:1,2-diacylglycerol 3-beta-galactosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR   InterPro; IPR009695; Diacylglyc_glucosyltr_N.
DR   InterPro; IPR007235; Glyco_trans_28_C.
DR   Pfam; PF04101; Glyco_tran_28_C; 1.
DR   Pfam; PF06925; MGDG_synth; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Glycosyltransferase; Membrane; Plastid; Reference proteome;
KW   Transferase; Transit peptide.
FT   TRANSIT         1..42
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           43..469
FT                   /note="Probable monogalactosyldiacylglycerol synthase 2,
FT                   chloroplastic"
FT                   /id="PRO_0000349429"
SQ   SEQUENCE   469 AA;  52123 MW;  391BEC5A85A476FF CRC64;
     MVISVATPRR SIRDAVLGGV LGAGGRQLYQ PLRCAFYDGA AGGGLTAALS EDGAEGGVPL
     PCGRKTAAAK NVLILMSDTG GGHRASAEAL RDAFRLEFGD AYQVFVRDLG KEYGGWPLND
     MERSYKFMIR HVRLWKVAFH GTSPRWVHGM YLAALAYFYA NEVVAGIMRY NPDIIISVHP
     LMQHIPLWVL KWQSLHPKVP FVTVITDLNT CHPTWFHHGV TRCYCPSAEV AKRALLRGLE
     PSQIRVYGLP IRPSFCRAVL DKDELRKELD MDPDLPAVLL MGGGEGMGPV EETARALSDE
     LYDRRRRRPV GQIVVICGRN QVLRSTLQSS RWNVPVKIRG FEKQMEKWMG ACDCIITKAG
     PGTIAEALIR GLPIILNDFI PGQEVGNVPY VVDNGAGVFS KDPREAARQV ARWFTTHTNE
     LRRYSLNALK LAQPEAVFDI VKDIHKLQQQ PATVTRIPYS LTSSFSYSI