ID   MGDG_CUCSA              Reviewed;         525 AA.
AC   P93115;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Monogalactosyldiacylglycerol synthase, chloroplastic;
DE            Short=CsMGD1;
DE            EC=2.4.1.46;
DE   Flags: Precursor;
OS   Cucumis sativus (Cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3659;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND PROTEIN SEQUENCE OF 104-108;
RP   137-148; 197-199; 203-228; 265-275; 285-298; 366-383; 389-397; 406-410 AND
RP   506-525.
RC   STRAIN=cv. Aonagajibai;
RX   PubMed=8990209; DOI=10.1073/pnas.94.1.333;
RA   Shimojima M., Ohta H., Iwamatsu A., Masuda T., Shioi Y., Takamiya K.;
RT   "Cloning of the gene for monogalactosyldiacylglycerol synthase and its
RT   evolutionary origin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:333-337(1997).
RN   [2]
RP   INDUCTION BY LIGHT AND CYTOKININ.
RX   PubMed=11171189; DOI=10.1042/bst0280738;
RA   Yamaryo Y., Kanai D., Awai K., Masuda T., Shimada H., Takamiya K., Ohta H.;
RT   "Transcriptional regulation by light and phytohormones of the MGD gene in
RT   cucumber.";
RL   Biochem. Soc. Trans. 28:738-740(2000).
RN   [3]
RP   INDUCTION BY LIGHT AND CYTOKININ.
RX   PubMed=12941877; DOI=10.1093/pcp/pcg110;
RA   Yamaryo Y., Kanai D., Awai K., Shimojima M., Masuda T., Shimada H.,
RA   Takamiya K., Ohta H.;
RT   "Light and cytokinin play a co-operative role in MGDG synthesis in greening
RT   cucumber cotyledons.";
RL   Plant Cell Physiol. 44:844-855(2003).
CC   -!- FUNCTION: Involved in the synthesis of the major structural component
CC       of photosynthetic membranes. {ECO:0000269|PubMed:8990209}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + UDP-alpha-D-galactose = a 1,2-
CC         diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + H(+) + UDP;
CC         Xref=Rhea:RHEA:14945, ChEBI:CHEBI:15378, ChEBI:CHEBI:17615,
CC         ChEBI:CHEBI:17815, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.46;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305}.
CC   -!- INDUCTION: By light and cytokinin. {ECO:0000269|PubMed:11171189,
CC       ECO:0000269|PubMed:12941877}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC       {ECO:0000305}.
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DR   EMBL; U62622; AAC49624.1; -; mRNA.
DR   PIR; T10478; T10478.
DR   RefSeq; NP_001292699.1; NM_001305770.1.
DR   AlphaFoldDB; P93115; -.
DR   SMR; P93115; -.
DR   STRING; 3659.XP_004138694.1; -.
DR   CAZy; GT28; Glycosyltransferase Family 28.
DR   GeneID; 101209030; -.
DR   KEGG; csv:101209030; -.
DR   eggNOG; ENOG502QPXV; Eukaryota.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046509; F:1,2-diacylglycerol 3-beta-galactosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR   InterPro; IPR009695; Diacylglyc_glucosyltr_N.
DR   InterPro; IPR007235; Glyco_trans_28_C.
DR   Pfam; PF04101; Glyco_tran_28_C; 1.
DR   Pfam; PF06925; MGDG_synth; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Glycosyltransferase; Membrane;
KW   Plastid; Transferase; Transit peptide.
FT   TRANSIT         1..103
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000269|PubMed:8990209"
FT   CHAIN           104..525
FT                   /note="Monogalactosyldiacylglycerol synthase,
FT                   chloroplastic"
FT                   /id="PRO_0000349424"
SQ   SEQUENCE   525 AA;  57863 MW;  74FE586082EC48BA CRC64;
     MRNPSTVVQE NGSVSDFISQ LGYFAFSSRF LNLNSEGCSG SSSHSLYLNG FENYRCVKRP
     PRSGASLSLS SRGSSSLRRF VNEFNNVIKF HCHKPPLGFA SLGGVSDETN GIRDDGFGVS
     QDGALPLNKI EAENPKRVLI LMSDTGGGHR ASAEAIKAAF NEEFGNNYQV FITDLWTDHT
     PWPFNQLPRS YNFLVKHGTL WKMTYYVTAP KVIHQSNFAA TSTFIAREVA KGLMKYRPDI
     IISVHPLMQH VPIRILRSKG LLNKIVFTTV VTDLSTCHPT WFHKLVTRCY CPSTEVAKRA
     LTAGLQPSKL KVFGLPVRPS FVKPIRPKIE LRKELGMDEN LPAVLLMGGG EGMGPIEATA
     KALSKALYDE NHGEPIGQVL VICGHNKKLA GRLRSIDWKV PVQVKGFVTK MEECMGACDC
     IITKAGPGTI AEAMIRGLPI ILNDYIAGQE AGNVPYVVEN GCGKFSKSPK EIANIVAKWF
     GPKADELLIM SQNALRLARP DAVFKIVHDL HELVKQRSFV PQYSG