ID   MGDG_TOBAC              Reviewed;         535 AA.
AC   Q9FZL3;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Probable monogalactosyldiacylglycerol synthase, chloroplastic;
DE            Short=NtMGD1;
DE            EC=2.4.1.46;
DE   AltName: Full=MGDG synthase type A;
DE   Flags: Precursor;
GN   Name=MGD A;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11553816; DOI=10.1073/pnas.181331498;
RA   Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H.,
RA   Takamiya K., Ohta H., Joyard J.;
RT   "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3
RT   plants, differentially mediate galactolipid syntheses in photosynthetic and
RT   nonphotosynthetic tissues in Arabidopsis thaliana.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001).
CC   -!- FUNCTION: Involved in the synthesis of the major structural component
CC       of photosynthetic membranes. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + UDP-alpha-D-galactose = a 1,2-
CC         diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + H(+) + UDP;
CC         Xref=Rhea:RHEA:14945, ChEBI:CHEBI:15378, ChEBI:CHEBI:17615,
CC         ChEBI:CHEBI:17815, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.46;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC       {ECO:0000305}.
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DR   EMBL; AB047476; BAB11980.1; -; mRNA.
DR   RefSeq; NP_001312377.1; NM_001325448.1.
DR   AlphaFoldDB; Q9FZL3; -.
DR   SMR; Q9FZL3; -.
DR   STRING; 4097.Q9FZL3; -.
DR   CAZy; GT28; Glycosyltransferase Family 28.
DR   GeneID; 107787940; -.
DR   KEGG; nta:107787940; -.
DR   OMA; VICTHPF; -.
DR   PhylomeDB; Q9FZL3; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046509; F:1,2-diacylglycerol 3-beta-galactosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR   InterPro; IPR009695; Diacylglyc_glucosyltr_N.
DR   InterPro; IPR007235; Glyco_trans_28_C.
DR   Pfam; PF04101; Glyco_tran_28_C; 1.
DR   Pfam; PF06925; MGDG_synth; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Glycosyltransferase; Membrane; Plastid; Reference proteome;
KW   Transferase; Transit peptide.
FT   TRANSIT         1..113
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           114..535
FT                   /note="Probable monogalactosyldiacylglycerol synthase,
FT                   chloroplastic"
FT                   /id="PRO_0000349425"
SQ   SEQUENCE   535 AA;  59589 MW;  B1B2067E86EDE477 CRC64;
     MMQHSSSVTQ EPTNPFGFVS QVGSFVFNNS CSRVSKSNFT LDSYSNLLSN YLYFDCNVKK
     DSLNHKNKPK ASPLLSLSIS NKSASSFSRV LFQFNKAIRF HCEKIPLGFA SVGVNCGESN
     GVREEGSVVE NEGIPDNGVE SEPPKKVLIL MSDTGGGHRA SAEAIRSAFN EEFGDKYQVF
     ITDLWTEHTP WPFNQLPRSY NFLVKHGSLW RMTYYATAPR LVHQTNFAAT STFIAREVAK
     GLMKYQPDII ISVHPLMQHV PLRILRSKGL LKKIIFTTVI TDLSTCHPTW FHKLVTRCYC
     PSEEVAKRAL RAGLKPYQLK VYGLPVRPSF VKPVPPKVEL RKELGMEEHL PAVLLMGGGE
     GMGPIEATAR ALGDALYDEI HGEPIGQVLV ICGRNKKLFN RLTSVQWKIP VQVKGFVTKM
     EECMGACDCI ITKAGPGTIA EAVIRGLPII LNDYIAGQEA GNVPYVIENG CGKFSKSPKK
     IANIVAQWFG PRQDELRIMS QNALRLARPD AVFKIVHDMH ELVRQRNFEP QCCPA