MGGS_THEMA
ID MGGS_THEMA Reviewed; 427 AA.
AC Q9X0V7; G4FEB6;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Mannosylglucosylglycerate synthase {ECO:0000303|PubMed:20061481};
DE EC=2.4.1.- {ECO:0000269|PubMed:20061481};
GN Name=mggS {ECO:0000303|PubMed:20061481};
GN OrderedLocusNames=TM_1230 {ECO:0000312|EMBL:AAD36305.1};
GN ORFNames=THEMA_08180 {ECO:0000312|EMBL:AHD18863.1},
GN Tmari_1237 {ECO:0000312|EMBL:AGL50161.1};
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=23637642; DOI=10.1371/journal.pgen.1003485;
RA Latif H., Lerman J.A., Portnoy V.A., Tarasova Y., Nagarajan H.,
RA Schrimpe-Rutledge A.C., Smith R.D., Adkins J.N., Lee D.H., Qiu Y.,
RA Zengler K.;
RT "The genome organization of Thermotoga maritima reflects its lifestyle.";
RL PLoS Genet. 9:E1003485-E1003485(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RG DOE Joint Genome Institute;
RA Noll K.M., Huntemann M., Han J., Chen A., Kyrpides N., Mavromatis K.,
RA Markowitz V., Palaniappan K., Ivanova N., Schaumberg A., Pati A.,
RA Liolios K., Nordberg H.P., Cantor M.N., Hua S.X., Woyke T.;
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, COFACTOR, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=20061481; DOI=10.1128/jb.01424-09;
RA Fernandes C., Mendes V., Costa J., Empadinhas N., Jorge C., Lamosa P.,
RA Santos H., da Costa M.S.;
RT "Two alternative pathways for the synthesis of the rare compatible solute
RT mannosylglucosylglycerate in Petrotoga mobilis.";
RL J. Bacteriol. 192:1624-1633(2010).
CC -!- FUNCTION: Catalyzes the synthesis of mannosylglucosylglycerate (MGG)
CC from glucosylglycerate (GG) and GDP-mannose.
CC {ECO:0000269|PubMed:20061481}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R)-2-O-(alpha-D-glucopyranosyl)-glycerate + GDP-alpha-D-
CC mannose = (2R)-2-O-[alpha-D-mannopyranosyl-(1->2)-alpha-D-
CC glucopyranosyl]-glycerate + GDP + H(+); Xref=Rhea:RHEA:47728,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57527, ChEBI:CHEBI:58189,
CC ChEBI:CHEBI:62510, ChEBI:CHEBI:87836;
CC Evidence={ECO:0000269|PubMed:20061481};
CC -!- COFACTOR:
CC Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC Evidence={ECO:0000269|PubMed:20061481};
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:20061481};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:20061481};
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000269|PubMed:20061481};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 7.0. {ECO:0000269|PubMed:20061481};
CC Temperature dependence:
CC Optimum temperature is 60 degrees Celsius.
CC {ECO:0000269|PubMed:20061481};
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC {ECO:0000305}.
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DR EMBL; AE000512; AAD36305.1; -; Genomic_DNA.
DR EMBL; CP004077; AGL50161.1; -; Genomic_DNA.
DR EMBL; CP007013; AHD18863.1; -; Genomic_DNA.
DR PIR; G72278; G72278.
DR RefSeq; NP_229035.1; NC_000853.1.
DR RefSeq; WP_004080052.1; NZ_CP011107.1.
DR AlphaFoldDB; Q9X0V7; -.
DR SMR; Q9X0V7; -.
DR STRING; 243274.THEMA_08180; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR EnsemblBacteria; AAD36305; AAD36305; TM_1230.
DR EnsemblBacteria; AGL50161; AGL50161; Tmari_1237.
DR KEGG; tma:TM1230; -.
DR KEGG; tmi:THEMA_08180; -.
DR KEGG; tmm:Tmari_1237; -.
DR KEGG; tmw:THMA_1256; -.
DR PATRIC; fig|243274.17.peg.1235; -.
DR eggNOG; COG0438; Bacteria.
DR InParanoid; Q9X0V7; -.
DR OMA; VEHNFEV; -.
DR OrthoDB; 508915at2; -.
DR BioCyc; MetaCyc:MON-16144; -.
DR Proteomes; UP000008183; Chromosome.
DR Proteomes; UP000013901; Chromosome.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR InterPro; IPR001296; Glyco_trans_1.
DR Pfam; PF00534; Glycos_transf_1; 1.
PE 1: Evidence at protein level;
KW Cobalt; Glycosyltransferase; Magnesium; Manganese; Nickel;
KW Reference proteome; Transferase.
FT CHAIN 1..427
FT /note="Mannosylglucosylglycerate synthase"
FT /id="PRO_0000431558"
SQ SEQUENCE 427 AA; 49702 MW; C3C1662ACA7D5AA5 CRC64;
MKIALIHYRG GLMDGVSLEM EKWKKVLTKM GHEVHIVAEN KKEGVDLTLK EIGFENPDFE
RVNRNFFGGI KDFLSEKEFL DFLKEKEEEL FHILNEALKD YDLIVPNNIW SLGLFPSLGL
ALSRLEKNFV AHHHDFWWER KHLIPENRRF REILDKHFPP DLPNVKHVVI NTIAQRELKR
RRNIDSVVVP NVMDFSSPIT SEEMYHRVRE ELQIAPGTIV ALQATRIDRR KTIELSIDVV
SLLKETLTSK KEADLYNGER YSGEVILLFS GICEDEEYLK ELKEYASSKG VSLLVLSEEV
RKNTSLFWKL YNAADFVTYP SILEGWGNQL LEAIAAKKPV VLFEYEVFKS DIKPAGLKYV
SLGDRCFREN GLVKVDERIL KKAVEEISRL LFDPSLYRET VEHNFEVGKR HFSLERLEDI
LSREVLP
