ID   MGLA_CLOPS              Reviewed;         515 AA.
AC   Q0ST95;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Galactose/methyl galactoside import ATP-binding protein MglA {ECO:0000255|HAMAP-Rule:MF_01717};
DE            EC=7.5.2.11 {ECO:0000255|HAMAP-Rule:MF_01717};
GN   Name=mglA {ECO:0000255|HAMAP-Rule:MF_01717}; OrderedLocusNames=CPR_1342;
OS   Clostridium perfringens (strain SM101 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=289380;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SM101 / Type A;
RX   PubMed=16825665; DOI=10.1101/gr.5238106;
RA   Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T.,
RA   Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H.,
RA   Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A.,
RA   Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D.,
RA   Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J.,
RA   Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B.,
RA   Paulsen I.T.;
RT   "Skewed genomic variability in strains of the toxigenic bacterial pathogen,
RT   Clostridium perfringens.";
RL   Genome Res. 16:1031-1040(2006).
CC   -!- FUNCTION: Part of the ABC transporter complex MglABC involved in
CC       galactose/methyl galactoside import. Responsible for energy coupling to
CC       the transport system. {ECO:0000255|HAMAP-Rule:MF_01717}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-galactose(out) + H2O = ADP + D-galactose(in) + H(+) +
CC         phosphate; Xref=Rhea:RHEA:60156, ChEBI:CHEBI:4139, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=7.5.2.11; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01717};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MglA),
CC       two transmembrane proteins (MglC) and a solute-binding protein (MglB).
CC       {ECO:0000255|HAMAP-Rule:MF_01717}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01717};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01717}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Galactose/methyl galactoside importer (TC 3.A.1.2.3) family.
CC       {ECO:0000255|HAMAP-Rule:MF_01717}.
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DR   EMBL; CP000312; ABG86948.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q0ST95; -.
DR   SMR; Q0ST95; -.
DR   EnsemblBacteria; ABG86948; ABG86948; CPR_1342.
DR   KEGG; cpr:CPR_1342; -.
DR   OMA; RIDHKAT; -.
DR   Proteomes; UP000001824; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043211; F:ABC-type carbohydrate transporter activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015862; ABC_transpr_MglA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR43790:SF7; PTHR43790:SF7; 1.
DR   Pfam; PF00005; ABC_tran; 2.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
DR   PROSITE; PS51260; MGLA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding; Repeat;
KW   Sugar transport; Translocase; Transport.
FT   CHAIN           1..515
FT                   /note="Galactose/methyl galactoside import ATP-binding
FT                   protein MglA"
FT                   /id="PRO_0000261361"
FT   DOMAIN          8..243
FT                   /note="ABC transporter 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01717"
FT   DOMAIN          254..499
FT                   /note="ABC transporter 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01717"
FT   BINDING         40..47
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01717"
SQ   SEQUENCE   515 AA;  57625 MW;  5B770438BFA75B9A CRC64;
     MKDSSNLLEM RNISKEFPGV KALDNVTLKV KKGSVHALMG ENGAGKSTLM KCLFGIYHPN
     SGEIFISGQK VQFKNSKHAL DNGVSMVHQE LNQVRERNVM DNLWLGRYPK KGLFIDEKKM
     YDETEKIFKD LDINVNPRDK VSTLSVSQMQ MVEIAKAVSY KSKIIVMDEP TSSLTEKEVS
     HLFKIINKLR KQGISIIYIS HKMEEILEIS DEVTIMRDGK WIATEKASDL TMDLIIKLMV
     GRELTDRFPK KDHIPKETIL EVNNLSDAKN ELKNVSFKLR KGEILGIAGL VGAKRTETLE
     TLFGLREKGS GDIILHGKKV DNSKPFKAMQ NGFALVTEER RQTGIFGKLP IDFNSIIANI
     DSYKTSTGLL SNGRISKDTQ WVINSMKVKT PSQKTLIGSL SGGNQQKIVI GKWLLRKPEI
     LLLDEPTRGI DVGAKFEIYQ LINELAKEDK GIIMVSSEMP ELLGVCDRIL VMSNGMISGI
     VNANQTTQEE IMHLSAKYLS VTGGVNNANQ IKEKV