MGLA_SALTY
ID MGLA_SALTY Reviewed; 506 AA.
AC P23924;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Galactose/methyl galactoside import ATP-binding protein MglA {ECO:0000255|HAMAP-Rule:MF_01717};
DE EC=7.5.2.11 {ECO:0000255|HAMAP-Rule:MF_01717};
GN Name=mglA {ECO:0000255|HAMAP-Rule:MF_01717}; OrderedLocusNames=STM2189;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=8675022; DOI=10.1016/0378-1119(96)00072-8;
RA Stamm L.V., Young N.R., Frye J.G.;
RT "Sequences of the Salmonella typhimurium mglA and mglC genes.";
RL Gene 171:131-132(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
RC STRAIN=LT2;
RX PubMed=3146019; DOI=10.1007/bf00330498;
RA Benner-Luger D., Boos W.;
RT "The mglB sequence of Salmonella typhimurium LT2; promoter analysis by gene
RT fusions and evidence for a divergently oriented gene coding for the mgl
RT repressor.";
RL Mol. Gen. Genet. 214:579-587(1988).
RN [4]
RP SUBCELLULAR LOCATION, AND FUNCTION IN GALACTOSE TRANSPORT.
RX PubMed=3924896; DOI=10.1128/jb.163.1.37-45.1985;
RA Mueller N., Heine H.-G., Boos W.;
RT "Characterization of the Salmonella typhimurium mgl operon and its gene
RT products.";
RL J. Bacteriol. 163:37-45(1985).
RN [5]
RP BIOPHYSICOCHEMICAL PROPERTIES, FUNCTION IN GALACTOSE TRANSPORT, ATPASE
RP ACTIVITY, AND ACTIVITY REGULATION.
RX PubMed=8387496; DOI=10.1016/s0021-9258(18)98374-2;
RA Richarme G., El Yaagoubi A., Kohiyama M.;
RT "The MglA component of the binding protein-dependent galactose transport
RT system of Salmonella typhimurium is a galactose-stimulated ATPase.";
RL J. Biol. Chem. 268:9473-9477(1993).
CC -!- FUNCTION: Part of the ABC transporter complex MglABC involved in
CC galactose/methyl galactoside import. Responsible for energy coupling to
CC the transport system (Probable). {ECO:0000305|PubMed:3924896,
CC ECO:0000305|PubMed:8387496}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-galactose(out) + H2O = ADP + D-galactose(in) + H(+) +
CC phosphate; Xref=Rhea:RHEA:60156, ChEBI:CHEBI:4139, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=7.5.2.11; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01717};
CC -!- ACTIVITY REGULATION: Stimulated 3-fold by galactose and inhibited by
CC vanadate, N-ethylmaleimide, and 5-methoxyindole-2-carboxylic acid.
CC {ECO:0000269|PubMed:8387496}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=60 uM for ATP {ECO:0000269|PubMed:8387496};
CC Vmax=140 nmol/min/mg enzyme {ECO:0000269|PubMed:8387496};
CC pH dependence:
CC Optimum pH is about 8. {ECO:0000269|PubMed:8387496};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MglA),
CC two transmembrane proteins (MglC) and a solute-binding protein (MglB).
CC {ECO:0000255|HAMAP-Rule:MF_01717}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:3924896};
CC Peripheral membrane protein {ECO:0000305|PubMed:3924896}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC Galactose/methyl galactoside importer (TC 3.A.1.2.3) family.
CC {ECO:0000255|HAMAP-Rule:MF_01717}.
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DR EMBL; U40492; AAC44149.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21093.1; -; Genomic_DNA.
DR PIR; S29391; S29391.
DR RefSeq; NP_461134.1; NC_003197.2.
DR RefSeq; WP_000535907.1; NC_003197.2.
DR AlphaFoldDB; P23924; -.
DR SMR; P23924; -.
DR STRING; 99287.STM2189; -.
DR PaxDb; P23924; -.
DR EnsemblBacteria; AAL21093; AAL21093; STM2189.
DR GeneID; 1253711; -.
DR KEGG; stm:STM2189; -.
DR PATRIC; fig|99287.12.peg.2316; -.
DR HOGENOM; CLU_000604_92_3_6; -.
DR OMA; IKMLSGA; -.
DR PhylomeDB; P23924; -.
DR BioCyc; SENT99287:STM2189-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043211; F:ABC-type carbohydrate transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015862; ABC_transpr_MglA.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR43790:SF7; PTHR43790:SF7; 1.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
DR PROSITE; PS51260; MGLA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Repeat; Sugar transport;
KW Translocase; Transport.
FT CHAIN 1..506
FT /note="Galactose/methyl galactoside import ATP-binding
FT protein MglA"
FT /id="PRO_0000092513"
FT DOMAIN 14..249
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01717"
FT DOMAIN 259..506
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01717"
FT BINDING 46..53
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01717"
SQ SEQUENCE 506 AA; 56477 MW; F5B709311B0E6168 CRC64;
MGSTISPPSG EYLLEMRGIN KSFPGVKALD NVNLNVRPHS IHALMGENGA GKSTLLKCLF
GIYQKDSGSI VFQGKEVDFH SAKEALENGI SMVHQELNLV LQRSVMDNMW LGRYPTKGMF
VDQDKMYQDT KAIFDELDID IDPRARVGTL SVSQMQMIEI AKAFSYNAKI VIMDEPTSSL
TEKEVNHLFT IIRKLKERGC GIVYISHKME EIFQLCDEIT ILRDGQWIAT QPLEGLDMDK
IIAMMVGRSL NQRFPDKENK PGDVILEVRH LTSLRQPSIR DVSFDLHKGE ILGIAGLVGA
KRTDIVETLF GIREKSSGTI TLHGKKINNH TANEAINHGF ALVTEERRST GIYAYLDIGF
NSLISNIRNY KNKVGLLDNS RMKSDTQWVI DSMRVKTPGH RTQIGSLSGG NQQKVIIGRW
LLTQPEILML DEPTRGIDVG AKFEIYQLIA ELAKKGKGII IISSEMPELL GITDRILVMS
NGLVSGIVDT KTTTQNEILR LASLHL
