MGME1_MOUSE
ID MGME1_MOUSE Reviewed; 338 AA.
AC Q9CXC3; A2ANA5; Q3TIC0; Q543Z8;
DT 25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Mitochondrial genome maintenance exonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03030};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_03030};
DE Flags: Precursor;
GN Name=Mgme1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and DBA/2J; TISSUE=Cerebellum, Embryonic lung, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION.
RX PubMed=23434322; DOI=10.1016/j.cell.2013.02.004;
RA Spruijt C.G., Gnerlich F., Smits A.H., Pfaffeneder T., Jansen P.W.,
RA Bauer C., Munzel M., Wagner M., Muller M., Khan F., Eberl H.C.,
RA Mensinga A., Brinkman A.B., Lephikov K., Muller U., Walter J., Boelens R.,
RA van Ingen H., Leonhardt H., Carell T., Vermeulen M.;
RT "Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized
RT derivatives.";
RL Cell 152:1146-1159(2013).
CC -!- FUNCTION: Metal-dependent single-stranded DNA (ssDNA) exonuclease
CC involved in mitochondrial genome maintenance. Has preference for 5'-3'
CC exonuclease activity but is also capable of endoduclease activity on
CC linear substrates. Necessary for maintenance of proper 7S DNA levels.
CC Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the
CC processing of displaced DNA containing Okazaki fragments during RNA-
CC primed DNA synthesis on the lagging strand or via processing of DNA
CC flaps during long-patch base excision repair (By similarity).
CC Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in
CC stem cells. {ECO:0000255|HAMAP-Rule:MF_03030,
CC ECO:0000269|PubMed:23434322}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03030}.
CC -!- SIMILARITY: Belongs to the MGME1 family. {ECO:0000255|HAMAP-
CC Rule:MF_03030}.
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DR EMBL; AK018387; BAB31189.1; -; mRNA.
DR EMBL; AK031535; BAC27439.1; -; mRNA.
DR EMBL; AK043140; BAC31474.1; -; mRNA.
DR EMBL; AK049565; BAC33813.1; -; mRNA.
DR EMBL; AK159286; BAE34962.1; -; mRNA.
DR EMBL; AK167919; BAE39926.1; -; mRNA.
DR EMBL; AL808123; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC063088; AAH63088.1; -; mRNA.
DR CCDS; CCDS16816.1; -.
DR RefSeq; NP_001276559.1; NM_001289630.1.
DR RefSeq; NP_001276560.1; NM_001289631.1.
DR RefSeq; NP_083260.1; NM_028984.5.
DR RefSeq; XP_006500365.1; XM_006500302.2.
DR AlphaFoldDB; Q9CXC3; -.
DR SMR; Q9CXC3; -.
DR STRING; 10090.ENSMUSP00000028910; -.
DR PhosphoSitePlus; Q9CXC3; -.
DR EPD; Q9CXC3; -.
DR MaxQB; Q9CXC3; -.
DR PaxDb; Q9CXC3; -.
DR PeptideAtlas; Q9CXC3; -.
DR PRIDE; Q9CXC3; -.
DR ProteomicsDB; 295938; -.
DR Antibodypedia; 48613; 81 antibodies from 18 providers.
DR DNASU; 74528; -.
DR Ensembl; ENSMUST00000028910; ENSMUSP00000028910; ENSMUSG00000027424.
DR Ensembl; ENSMUST00000110027; ENSMUSP00000105654; ENSMUSG00000027424.
DR Ensembl; ENSMUST00000110028; ENSMUSP00000105655; ENSMUSG00000027424.
DR GeneID; 74528; -.
DR KEGG; mmu:74528; -.
DR UCSC; uc008mqs.2; mouse.
DR CTD; 92667; -.
DR MGI; MGI:1921778; Mgme1.
DR VEuPathDB; HostDB:ENSMUSG00000027424; -.
DR eggNOG; ENOG502QVKE; Eukaryota.
DR GeneTree; ENSGT00390000003349; -.
DR HOGENOM; CLU_068902_0_0_1; -.
DR InParanoid; Q9CXC3; -.
DR OMA; QLFYLER; -.
DR OrthoDB; 1513785at2759; -.
DR PhylomeDB; Q9CXC3; -.
DR TreeFam; TF320375; -.
DR BioGRID-ORCS; 74528; 1 hit in 108 CRISPR screens.
DR ChiTaRS; Mgme1; mouse.
DR PRO; PR:Q9CXC3; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9CXC3; protein.
DR Bgee; ENSMUSG00000027424; Expressed in interventricular septum and 187 other tissues.
DR Genevisible; Q9CXC3; MM.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0008297; F:single-stranded DNA exodeoxyribonuclease activity; ISS:UniProtKB.
DR GO; GO:0043504; P:mitochondrial DNA repair; ISS:UniProtKB.
DR GO; GO:0006264; P:mitochondrial DNA replication; ISO:MGI.
DR GO; GO:0000002; P:mitochondrial genome maintenance; ISS:UniProtKB.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_03030; MGME1; 1.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR Pfam; PF12705; PDDEXK_1; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA repair; Exonuclease; Hydrolase; Mitochondrion; Nuclease;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..64
FT /note="Mitochondrion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03030"
FT CHAIN 65..338
FT /note="Mitochondrial genome maintenance exonuclease 1"
FT /id="PRO_0000079440"
FT ACT_SITE 235
FT /evidence="ECO:0000250"
FT ACT_SITE 248
FT /evidence="ECO:0000250"
FT ACT_SITE 250
FT /evidence="ECO:0000250"
FT CONFLICT 213
FT /note="I -> M (in Ref. 1; BAE39926)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 338 AA; 38391 MW; E29537429443DFD3 CRC64;
MKLPLTFCRL LSRLNRFSVK ASPPVSFSTF SYLCSQKKKN SYEAVDQAKY SRLVRSVLSR
GPAQTPESLF KEDDVLYGPV SKHKAAEPEP QARVPQHCFP IFNEERTGKP HTDASSSPLK
IPLQRNSIPS VTRILQQTMP PEQSFFLERW KERMVLELGE DGFAEYTSNV FLQGKQFHKA
LESILSPQEN LTGGEEHPQC GYIESIQHIL TEISGVQALE SAVQHEALKY VGLLDCVAEY
RGKLCVIDWK TSEKPKPLIR NTYDNPLQVV AYMGAVNHDA HYSFQVQCGL IVVAYKDGSP
AHPHFMDEEL CSKYWAKWLL RLEEYTEKQK NLSAPEPA
