MGN2_MOUSE
ID MGN2_MOUSE Reviewed; 146 AA.
AC Q9CQL1; Q78Y23;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=Protein mago nashi homolog 2;
GN Name=Magohb; Synonyms=Magoh-rs1, Magoh2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Pancreas, Small intestine, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY, AND INDUCTION BY LIPOPOLYSACCHARIDE.
RX PubMed=23917022; DOI=10.4161/rna.25827;
RA Singh K.K., Wachsmuth L., Kulozik A.E., Gehring N.H.;
RT "Two mammalian MAGOH genes contribute to exon junction complex composition
RT and nonsense-mediated decay.";
RL RNA Biol. 10:1291-1298(2013).
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC spliceosome. Plays a redundant role with MAGOH in the exon junction
CC complex and in the nonsense-mediated decay (NMD) pathway.
CC {ECO:0000250|UniProtKB:Q96A72}.
CC -!- SUBUNIT: Component of the pre-catalytic, catalytic and post-catalytic
CC spliceosome complexes. Heterodimer with RBM8A. Core component of the
CC mRNA splicing-dependent exon junction complex (EJC); the core complex
CC contains CASC3, EIF4A3, MAGOH or MAGOHB, and RBM8A.
CC {ECO:0000250|UniProtKB:Q96A72}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96A72}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Detected in brain, heart, liver, lung,
CC spleen and testis. {ECO:0000269|PubMed:23917022}.
CC -!- INDUCTION: In macrophages, strongly induced by bacterial
CC lipopolysaccharide (LPS). Levels reach a maximum 6 hours after exposure
CC to LPS and are lower, but still much increased after 12 and 24 hours.
CC {ECO:0000269|PubMed:23917022}.
CC -!- SIMILARITY: Belongs to the mago nashi family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB24516.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK006303; BAB24516.2; ALT_INIT; mRNA.
DR EMBL; AK007769; BAB25244.1; -; mRNA.
DR EMBL; AK008200; BAB25528.1; -; mRNA.
DR EMBL; AK013310; BAB28784.1; -; mRNA.
DR EMBL; BC027505; AAH27505.1; -; mRNA.
DR RefSeq; NP_001314789.1; NM_001327860.1.
DR RefSeq; NP_079840.2; NM_025564.2.
DR AlphaFoldDB; Q9CQL1; -.
DR SMR; Q9CQL1; -.
DR ComplexPortal; CPX-681; Exon junction subcomplex Magohb-Y14.
DR ComplexPortal; CPX-683; Exon junction core complex, Magohb variant.
DR IntAct; Q9CQL1; 2.
DR STRING; 10090.ENSMUSP00000032307; -.
DR iPTMnet; Q9CQL1; -.
DR PhosphoSitePlus; Q9CQL1; -.
DR EPD; Q9CQL1; -.
DR MaxQB; Q9CQL1; -.
DR PaxDb; Q9CQL1; -.
DR PeptideAtlas; Q9CQL1; -.
DR PRIDE; Q9CQL1; -.
DR ProteomicsDB; 293469; -.
DR DNASU; 66441; -.
DR GeneID; 66441; -.
DR KEGG; mmu:66441; -.
DR CTD; 55110; -.
DR MGI; MGI:1913691; Magohb.
DR eggNOG; KOG3392; Eukaryota.
DR InParanoid; Q9CQL1; -.
DR OrthoDB; 1373136at2759; -.
DR PhylomeDB; Q9CQL1; -.
DR TreeFam; TF300128; -.
DR Reactome; R-MMU-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72187; mRNA 3'-end processing.
DR Reactome; R-MMU-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-MMU-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR BioGRID-ORCS; 66441; 4 hits in 75 CRISPR screens.
DR ChiTaRS; Magohb; mouse.
DR PRO; PR:Q9CQL1; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9CQL1; protein.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IC:ComplexPortal.
DR GO; GO:0035145; C:exon-exon junction complex; ISO:MGI.
DR GO; GO:1990501; C:exon-exon junction subcomplex mago-y14; ISO:MGI.
DR GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; ISS:UniProtKB.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006406; P:mRNA export from nucleus; IC:ComplexPortal.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISO:MGI.
DR GO; GO:0050684; P:regulation of mRNA processing; IC:ComplexPortal.
DR GO; GO:2000622; P:regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IC:ComplexPortal.
DR GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR CDD; cd11295; Mago_nashi; 1.
DR Gene3D; 3.30.1560.10; -; 1.
DR InterPro; IPR004023; Mago_nashi.
DR InterPro; IPR036605; Mago_nashi_sf.
DR PANTHER; PTHR12638; PTHR12638; 1.
DR Pfam; PF02792; Mago_nashi; 1.
DR SUPFAM; SSF89817; SSF89817; 1.
PE 2: Evidence at transcript level;
KW mRNA processing; mRNA splicing; mRNA transport; Nucleus;
KW Reference proteome; RNA-binding; Spliceosome; Transport.
FT CHAIN 1..146
FT /note="Protein mago nashi homolog 2"
FT /id="PRO_0000174148"
SQ SEQUENCE 146 AA; 17092 MW; 7C3BCAB1BE045BC3 CRC64;
MGSDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL
KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT TSKIGSLIDV NQSKDPEGLR
VFYYLVQDLK CLVFSLIGLH FKIKPI
