ID   MGN_BOVIN               Reviewed;         146 AA.
AC   Q3ZBV3;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Protein mago nashi homolog;
GN   Name=MAGOH;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC       spliceosome. Plays a redundant role with MAGOHB as core component of
CC       the exon junction complex (EJC) and in the nonsense-mediated decay
CC       (NMD) pathway. The EJC is a dynamic structure consisting of core
CC       proteins and several peripheral nuclear and cytoplasmic associated
CC       factors that join the complex only transiently either during EJC
CC       assembly or during subsequent mRNA metabolism. The EJC marks the
CC       position of the exon-exon junction in the mature mRNA for the gene
CC       expression machinery and the core components remain bound to spliced
CC       mRNAs throughout all stages of mRNA metabolism thereby influencing
CC       downstream processes including nuclear mRNA export, subcellular mRNA
CC       localization, translation efficiency and nonsense-mediated mRNA decay
CC       (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of
CC       EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a
CC       stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC
CC       key regulator PYM1 leading to EJC disassembly in the cytoplasm and
CC       translation enhancement of EJC-bearing spliced mRNAs by recruiting them
CC       to the ribosomal 48S preinitiation complex. Involved in the splicing
CC       modulation of BCL2L1/Bcl-X (and probably other apoptotic genes);
CC       specifically inhibits formation of proapoptotic isoforms; the function
CC       is different from the established EJC assembly.
CC       {ECO:0000250|UniProtKB:P61326}.
CC   -!- SUBUNIT: Heterodimer with RBM8A. Core component of the mRNA splicing-
CC       dependent exon junction complex (EJC); the core complex contains CASC3,
CC       EIF4A3, MAGOH or MAGOHB, and RBM8A. Interacts with PYM1; the
CC       interaction is direct and dissociates the EJC from spliced mRNAs.
CC       Identified in a complex composed of the EJC core, UPF3B and UPF2. The
CC       EJC core can also interact with UPF3A (in vitro). Identified in the
CC       spliceosome C complex. {ECO:0000250|UniProtKB:P61326}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P61326}. Nucleus
CC       speckle {ECO:0000250|UniProtKB:P61326}. Cytoplasm
CC       {ECO:0000250|UniProtKB:P61326}. Note=Detected in granule-like
CC       structures in the dendroplasm. Travels to the cytoplasm as part of the
CC       exon junction complex (EJC) bound to mRNA. Colocalizes with the core
CC       EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
CC       {ECO:0000250|UniProtKB:P61326, ECO:0000250|UniProtKB:Q27W02}.
CC   -!- SIMILARITY: Belongs to the mago nashi family. {ECO:0000305}.
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DR   EMBL; BC103092; AAI03093.1; -; mRNA.
DR   RefSeq; NP_001029417.1; NM_001034245.2.
DR   AlphaFoldDB; Q3ZBV3; -.
DR   SMR; Q3ZBV3; -.
DR   STRING; 9913.ENSBTAP00000004528; -.
DR   PaxDb; Q3ZBV3; -.
DR   GeneID; 505417; -.
DR   KEGG; bta:505417; -.
DR   CTD; 4116; -.
DR   eggNOG; KOG3392; Eukaryota.
DR   HOGENOM; CLU_109497_1_1_1; -.
DR   InParanoid; Q3ZBV3; -.
DR   OrthoDB; 1373136at2759; -.
DR   TreeFam; TF300128; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0035145; C:exon-exon junction complex; IBA:GO_Central.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR   CDD; cd11295; Mago_nashi; 1.
DR   Gene3D; 3.30.1560.10; -; 1.
DR   InterPro; IPR004023; Mago_nashi.
DR   InterPro; IPR036605; Mago_nashi_sf.
DR   PANTHER; PTHR12638; PTHR12638; 1.
DR   Pfam; PF02792; Mago_nashi; 1.
DR   SUPFAM; SSF89817; SSF89817; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cytoplasm; mRNA processing; mRNA splicing; mRNA transport;
KW   Nonsense-mediated mRNA decay; Nucleus; Reference proteome; RNA-binding;
KW   Spliceosome; Translation regulation; Transport.
FT   CHAIN           1..146
FT                   /note="Protein mago nashi homolog"
FT                   /id="PRO_0000253719"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:P61326"
SQ   SEQUENCE   146 AA;  17164 MW;  FFAD0B075E045875 CRC64;
     MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL
     KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT TSKIGSLIDV NQSKDPEGLR
     VFYYLVQDLK CLVFSLIGLH FKIKPI