MGN_CAEEL
ID MGN_CAEEL Reviewed; 152 AA.
AC P49029; A0A023T4K3; O16104; O45714;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein mago nashi homolog;
DE AltName: Full=Ce-mago;
GN Name=mag-1 {ECO:0000312|WormBase:R09B3.5};
GN ORFNames=R09B3.5 {ECO:0000312|WormBase:R09B3.5};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000312|EMBL:AHX83788.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=24416299; DOI=10.1371/journal.pone.0084842;
RA Gong P., Zhao M., He C.;
RT "Slow co-evolution of the MAGO and Y14 protein families is required for the
RT maintenance of their obligate heterodimerization mode.";
RL PLoS ONE 9:E84842-E84842(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 6-152.
RX PubMed=9272960; DOI=10.1242/dev.124.16.3197;
RA Newmark P.A., Mohr S.E., Gong L., Boswell R.E.;
RT "Mago nashi mediates the posterior follicle cell-to-oocyte signal to
RT organize axis formation in Drosophila.";
RL Development 124:3197-3207(1997).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=10656761; DOI=10.1006/dbio.1999.9593;
RA Li W., Boswell R., Wood W.B.;
RT "mag-1, a homolog of Drosophila mago nashi, regulates hermaphrodite germ-
RT line sex determination in Caenorhabditis elegans.";
RL Dev. Biol. 218:172-182(2000).
RN [5]
RP FUNCTION, INTERACTION WITH RNP-4, AND DISRUPTION PHENOTYPE.
RX PubMed=14706697; DOI=10.1016/j.mod.2003.11.003;
RA Kawano T., Kataoka N., Dreyfuss G., Sakamoto H.;
RT "Ce-Y14 and MAG-1, components of the exon-exon junction complex, are
RT required for embryogenesis and germline sexual switching in Caenorhabditis
RT elegans.";
RL Mech. Dev. 121:27-35(2004).
RN [6]
RP FUNCTION, IDENTIFICATION IN THE EXON JUNCTION COMPLEX, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=23149939; DOI=10.1128/mcb.01298-12;
RA Shiimori M., Inoue K., Sakamoto H.;
RT "A specific set of exon junction complex subunits is required for the
RT nuclear retention of unspliced RNAs in Caenorhabditis elegans.";
RL Mol. Cell. Biol. 33:444-456(2013).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=30279189; DOI=10.1534/g3.118.200744;
RA Gauvin T.J., Han B., Sun M.J., Griffin E.E.;
RT "PIE-1 Translation in the Germline Lineage Contributes to PIE-1 Asymmetry
RT in the Early Caenorhabditis elegans Embryo.";
RL G3 (Bethesda) 8:3791-3801(2018).
CC -!- FUNCTION: Core component of the splicing-dependent multiprotein exon
CC junction complex (EJC) deposited at splice junctions on mRNAs
CC (Probable). Positively regulates the nuclear export of spliced mRNAs
CC including the sex determination gene tra-2 (PubMed:23149939). Promotes
CC oogenesis and represses germline masculinization and spermatogenesis
CC (PubMed:10656761, PubMed:14706697, PubMed:23149939). Required for
CC embryonic development, possibly through positively regulating the
CC expression of pie-1 (PubMed:30279189). Not required for the spatial
CC patterning of pie-1 in embryos (PubMed:30279189).
CC {ECO:0000269|PubMed:10656761, ECO:0000269|PubMed:14706697,
CC ECO:0000269|PubMed:23149939, ECO:0000269|PubMed:30279189,
CC ECO:0000305|PubMed:23149939}.
CC -!- SUBUNIT: Heterodimer with rnp-4/RBM8A (PubMed:14706697). Part of the
CC mRNA splicing-dependent exon junction complex (EJC) complex; the core
CC complex contains CASC3, EIF4A3, mag-1/MAGOH and rnp-4/RBM8A (Probable).
CC {ECO:0000269|PubMed:14706697, ECO:0000305|PubMed:23149939}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P61326}.
CC -!- TISSUE SPECIFICITY: Expressed in the soma and germline of
CC hermaphrodites. {ECO:0000269|PubMed:10656761}.
CC -!- DEVELOPMENTAL STAGE: Expressed at all stages of development in
CC hermaphrodites (PubMed:10656761). More abundant in embryos than in
CC larvae and adults (PubMed:10656761). First expressed in embryos prior
CC to morphogenesis, and after morphogenesis begins, primarily expressed
CC in the head and tail regions (PubMed:10656761).
CC {ECO:0000269|PubMed:10656761}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in embryonic
CC lethality due to developmental arrest during morphogenesis
CC (PubMed:30279189). RNAi-mediated knockdown in the syncytial gonads of
CC adult hermaphrodites results in some inviable embryos and then
CC eventually no egg laying (PubMed:10656761). RNAi-mediated knockdown at
CC the L1 larval stage results in the accumulation of unspliced mRNAs
CC including ama-1 and ife-4 in the cytoplasm, and specifically in the
CC production of an irregular unspliced form of the sex determining
CC protein tra-2, which accumulates in the cytoplasm and inhibits the
CC regular tra-2 isoforms a and c reducing their expression and disrupting
CC their nuclear localization (PubMed:23149939). RNAi-mediated knockdown
CC in L4 hermaphrodite larvae, results in no viable embryos and egg laying
CC ceases due to masculinization of the germline (also called a mog
CC phenotype) with animals producing undifferentiated germ cells and sperm
CC instead of oocytes (PubMed:10656761, PubMed:14706697). In these
CC animals, sperm are distributed throughout the germline, including the
CC distal tip region (PubMed:10656761). RNAi-mediated knockdown does not
CC result in masculinization of the germline in some animals, but these
CC animals still do not produce oocytes with germlines appearing
CC disorganized (PubMed:10656761). RNAi-mediated knockdown reduces the
CC expression of pie-1 in P2 blastomeres (PubMed:30279189).
CC {ECO:0000269|PubMed:10656761, ECO:0000269|PubMed:14706697,
CC ECO:0000269|PubMed:23149939, ECO:0000269|PubMed:30279189}.
CC -!- SIMILARITY: Belongs to the mago nashi family. {ECO:0000305}.
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DR EMBL; KF051002; AHX83788.1; -; mRNA.
DR EMBL; BX284601; CAB03239.1; -; Genomic_DNA.
DR EMBL; AF007861; AAB66721.1; -; mRNA.
DR PIR; T24070; T24070.
DR PIR; T37461; T37461.
DR RefSeq; NP_493025.1; NM_060624.6.
DR AlphaFoldDB; P49029; -.
DR SMR; P49029; -.
DR BioGRID; 38476; 6.
DR DIP; DIP-26241N; -.
DR IntAct; P49029; 1.
DR STRING; 6239.R09B3.5; -.
DR iPTMnet; P49029; -.
DR EPD; P49029; -.
DR PaxDb; P49029; -.
DR PeptideAtlas; P49029; -.
DR EnsemblMetazoa; R09B3.5.1; R09B3.5.1; WBGene00003123.
DR GeneID; 173073; -.
DR KEGG; cel:CELE_R09B3.5; -.
DR UCSC; R09B3.5.2; c. elegans.
DR CTD; 173073; -.
DR WormBase; R09B3.5; CE16310; WBGene00003123; mag-1.
DR eggNOG; KOG3392; Eukaryota.
DR GeneTree; ENSGT00390000003156; -.
DR HOGENOM; CLU_109497_1_1_1; -.
DR InParanoid; P49029; -.
DR OMA; IRKEMWI; -.
DR OrthoDB; 1373136at2759; -.
DR PhylomeDB; P49029; -.
DR Reactome; R-CEL-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-CEL-72187; mRNA 3'-end processing.
DR Reactome; R-CEL-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-CEL-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:P49029; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00003123; Expressed in embryo and 4 other tissues.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0035145; C:exon-exon junction complex; IBA:GO_Central.
DR GO; GO:0040022; P:feminization of hermaphroditic germ-line; IMP:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0071028; P:nuclear mRNA surveillance; IMP:UniProtKB.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:1905881; P:positive regulation of oogenesis; IMP:UniProtKB.
DR GO; GO:1901046; P:positive regulation of oviposition; IMP:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR CDD; cd11295; Mago_nashi; 1.
DR Gene3D; 3.30.1560.10; -; 1.
DR InterPro; IPR004023; Mago_nashi.
DR InterPro; IPR036605; Mago_nashi_sf.
DR PANTHER; PTHR12638; PTHR12638; 1.
DR Pfam; PF02792; Mago_nashi; 1.
DR SUPFAM; SSF89817; SSF89817; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; mRNA processing; mRNA splicing;
KW mRNA transport; Nucleus; Oogenesis; Reference proteome; Transport.
FT CHAIN 1..152
FT /note="Protein mago nashi homolog"
FT /id="PRO_0000174151"
FT CONFLICT 41
FT /note="A -> G (in Ref. 3; AAB66721)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 152 AA; 17637 MW; EBA9B49487385E01 CRC64;
MSGEEEKAAD FYVRYYVGHK GKFGHEFLEF EFRPNGSLRY ANNSNYKNDT MIRKEATVSE
SVLSELKRII EDSEIMQEDD DNWPEPDKIG RQELEILYKN EHISFTTGKI GALADVNNSK
DPDGLRSFYY LVQDLKCLVF SLIGLHFKIK PI
