MGN_RAT
ID MGN_RAT Reviewed; 146 AA.
AC Q27W02;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Protein mago nashi homolog;
DE AltName: Full=Mago-nashi-like proliferation-associated protein;
GN Name=Magoh;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Hippocampus;
RX PubMed=16275927; DOI=10.1073/pnas.0503783102;
RA Glanzer J., Miyashiro K.Y., Sul J.-Y., Barrett L., Belt B., Haydon P.,
RA Eberwine J.;
RT "RNA splicing capability of live neuronal dendrites.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:16859-16864(2005).
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC spliceosome. Plays a redundant role with MAGOHB as core component of
CC the exon junction complex (EJC) and in the nonsense-mediated decay
CC (NMD) pathway. The EJC is a dynamic structure consisting of core
CC proteins and several peripheral nuclear and cytoplasmic associated
CC factors that join the complex only transiently either during EJC
CC assembly or during subsequent mRNA metabolism. The EJC marks the
CC position of the exon-exon junction in the mature mRNA for the gene
CC expression machinery and the core components remain bound to spliced
CC mRNAs throughout all stages of mRNA metabolism thereby influencing
CC downstream processes including nuclear mRNA export, subcellular mRNA
CC localization, translation efficiency and nonsense-mediated mRNA decay
CC (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of
CC EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a
CC stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC
CC key regulator PYM1 leading to EJC disassembly in the cytoplasm and
CC translation enhancement of EJC-bearing spliced mRNAs by recruiting them
CC to the ribosomal 48S preinitiation complex. Involved in the splicing
CC modulation of BCL2L1/Bcl-X (and probably other apoptotic genes);
CC specifically inhibits formation of proapoptotic isoforms; the function
CC is different from the established EJC assembly.
CC {ECO:0000250|UniProtKB:P61326}.
CC -!- SUBUNIT: Heterodimer with RBM8A. Core component of the mRNA splicing-
CC dependent exon junction complex (EJC); the core complex contains CASC3,
CC EIF4A3, MAGOH or MAGOHB, and RBM8A. Interacts with PYM1; the
CC interaction is direct and dissociates the EJC from spliced mRNAs.
CC Identified in a complex composed of the EJC core, UPF3B and UPF2. The
CC EJC core can also interact with UPF3A (in vitro). Identified in the
CC spliceosome C complex. {ECO:0000250|UniProtKB:P61326}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16275927}. Nucleus
CC speckle {ECO:0000269|PubMed:16275927}. Cytoplasm
CC {ECO:0000269|PubMed:16275927}. Note=Detected in granule-like structures
CC in the dendroplasm (PubMed:16275927). Travels to the cytoplasm as part
CC of the exon junction complex (EJC) bound to mRNA. Colocalizes with the
CC core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear
CC speckles (By similarity). {ECO:0000250|UniProtKB:P61326,
CC ECO:0000269|PubMed:16275927}.
CC -!- TISSUE SPECIFICITY: Expressed in primary neurons.
CC {ECO:0000269|PubMed:16275927}.
CC -!- SIMILARITY: Belongs to the mago nashi family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ359101; ABD46660.1; -; mRNA.
DR RefSeq; NP_001094006.1; NM_001100536.1.
DR AlphaFoldDB; Q27W02; -.
DR SMR; Q27W02; -.
DR STRING; 10116.ENSRNOP00000017421; -.
DR jPOST; Q27W02; -.
DR PaxDb; Q27W02; -.
DR PRIDE; Q27W02; -.
DR GeneID; 298385; -.
DR KEGG; rno:298385; -.
DR UCSC; RGD:1305174; rat.
DR CTD; 4116; -.
DR RGD; 1305174; Magoh.
DR VEuPathDB; HostDB:ENSRNOG00000012778; -.
DR eggNOG; KOG3392; Eukaryota.
DR HOGENOM; CLU_109497_1_1_1; -.
DR InParanoid; Q27W02; -.
DR OMA; VSECEIM; -.
DR OrthoDB; 1373136at2759; -.
DR PhylomeDB; Q27W02; -.
DR TreeFam; TF300128; -.
DR Reactome; R-RNO-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-RNO-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-RNO-72187; mRNA 3'-end processing.
DR Reactome; R-RNO-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-RNO-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:Q27W02; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000012778; Expressed in thymus and 20 other tissues.
DR Genevisible; Q27W02; RN.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0035145; C:exon-exon junction complex; ISO:RGD.
DR GO; GO:1990501; C:exon-exon junction subcomplex mago-y14; ISO:RGD.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISO:RGD.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0050684; P:regulation of mRNA processing; ISO:RGD.
DR GO; GO:2000622; P:regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISO:RGD.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR CDD; cd11295; Mago_nashi; 1.
DR Gene3D; 3.30.1560.10; -; 1.
DR InterPro; IPR004023; Mago_nashi.
DR InterPro; IPR036605; Mago_nashi_sf.
DR PANTHER; PTHR12638; PTHR12638; 1.
DR Pfam; PF02792; Mago_nashi; 1.
DR SUPFAM; SSF89817; SSF89817; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; mRNA processing; mRNA splicing; mRNA transport;
KW Nonsense-mediated mRNA decay; Nucleus; Reference proteome; RNA-binding;
KW Spliceosome; Translation regulation; Transport.
FT CHAIN 1..146
FT /note="Protein mago nashi homolog"
FT /id="PRO_0000378583"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P61326"
SQ SEQUENCE 146 AA; 17164 MW; FFAD0B075E045875 CRC64;
MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL
KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT TSKIGSLIDV NQSKDPEGLR
VFYYLVQDLK CLVFSLIGLH FKIKPI
