MGP3_DICDI
ID MGP3_DICDI Reviewed; 1337 AA.
AC Q55DK5;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=GTPase activating protein homolog 3;
DE AltName: Full=GTPase activating factor for raC protein AA;
DE AltName: Full=Rho GTPase-activating protein gacAA;
GN Name=mgp3; Synonyms=gacAA; ORFNames=DDB_G0269624;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP IDENTIFICATION.
RX PubMed=18334553; DOI=10.1242/jcs.021113;
RA Heath R.J., Insall R.H.;
RT "Dictyostelium MEGAPs: F-BAR domain proteins that regulate motility and
RT membrane tubulation in contractile vacuoles.";
RL J. Cell Sci. 121:1054-1064(2008).
CC -!- FUNCTION: Rho GTPase-activating protein involved in the signal
CC transduction pathway. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Contractile vacuole {ECO:0000250}.
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DR EMBL; AAFI02000005; EAL72162.1; -; Genomic_DNA.
DR RefSeq; XP_646126.1; XM_641034.1.
DR AlphaFoldDB; Q55DK5; -.
DR SMR; Q55DK5; -.
DR STRING; 44689.DDB0233874; -.
DR PaxDb; Q55DK5; -.
DR PRIDE; Q55DK5; -.
DR EnsemblProtists; EAL72162; EAL72162; DDB_G0269624.
DR GeneID; 8617075; -.
DR KEGG; ddi:DDB_G0269624; -.
DR dictyBase; DDB_G0269624; mgp3.
DR eggNOG; KOG2398; Eukaryota.
DR HOGENOM; CLU_258661_0_0_1; -.
DR InParanoid; Q55DK5; -.
DR OMA; YLCYLGG; -.
DR PRO; PR:Q55DK5; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0000331; C:contractile vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 1.20.1270.60; -; 1.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR031160; F_BAR.
DR InterPro; IPR001060; FCH_dom.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF00611; FCH; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00055; FCH; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF103657; SSF103657; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS51741; F_BAR; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasm; GTPase activation; Reference proteome; Vacuole.
FT CHAIN 1..1337
FT /note="GTPase activating protein homolog 3"
FT /id="PRO_0000380226"
FT DOMAIN 14..264
FT /note="F-BAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01077"
FT DOMAIN 422..611
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 279..328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 345..392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 612..689
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 733..781
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 794..821
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1041..1102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1114..1166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 830..859
FT /evidence="ECO:0000255"
FT COILED 1189..1219
FT /evidence="ECO:0000255"
FT COMPBIAS 290..305
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 306..328
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 615..689
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 794..817
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1337 AA; 150248 MW; 73393E0FD5FD8AF4 CRC64;
MTVDNSQIPN LNQPESFADL WDGYENVSKA TEKGVLLLKD ITKFFKKRIQ SEDEYSKTLS
KLVLKFEPMA PDGYIGPRLK NTWDQIKLET LTHSNHHENI CNNISTHIIE PIEGLIVDLE
QKMKSIHVDA EKSFIHYQES VAKLKKAKQN YDRLCKDSFE ITGVSKGETQ KVQKRAIKAA
QDVIKADKDY RAQINETNTS QKQFLTELIP KIMNDLQRLE MVRIHMVKSY FHRYFKSMES
TPQKFNTETE NLLNLINSIN NEDEIQDFVR KSKTTHKYPK PFEYEPYLDR FTTPPPPPPP
QISSPQLLSP RGDISIQHSS SSNSLPIFPT QHQLLSNKEK ADNNITNSLS LSSDSLQTNL
NNGNNGGNGN NGSNTPTKEK KPSSWGLKRF STSVSSTSDR KLLNKQIQSI GLAHNGGNIF
NCKIEDIMVA QKKKYPYLEI PFIVVFLKHK LVALDVFKTQ GIFRVPGNVV DINSLKKRFD
EGNYEVAPTE NVYTIASLLK LWLREITEPL FPLTVYDQCI ENSNKREKIF EIISSLPILN
QKTISYIIEL LQECGKSVNV EHNKMNFPNL AMVFSPCFLR CSHTDPNILL GNIFKEKEFV
QNIIEHFKPL QVNDSLEGPP SSSSSSSTSI NQSSIESPPT SPLRSSTNSN ANKSSTPIKP
SSPSQQQQQQ QQQQQSSTPS TPLSSTTNTN VKHNILSPIV EQQSPNNGKQ PIALTQTYSH
LNIGSVPLII NTVNNNNQNQ NQNQNQNQNQ DQNQNQSKQP IQSSNQTQQQ VSAPATPHTV
AVNPKINQIK AETITTPQKS IGDGNGLIGQ SPSAHLMSPS EVKRRSNAIY LEDQERCKQR
IDELHTQVNE LYSDITTIET TTYFAMQSSL LITKLTKSLE SFLTIDMWNL TLQDIKEAIE
KNKFVSPPPI NFKIPSKMPK LSPIELVQNE QSSELLRSWL SNVTLTVNRI NEYLCYLGGV
VIRIHSPDTL FAISNLFTDY DLSPQQNPKF VSLTLEQSTI FIQKTLALLE PLNPFTSDEL
NINVKVQDTI TPASFSPALI SDPDCNSPPT ISNTTNRLLN TSGSTDFSTT PLSSSPSTSS
TSLSTNNNNN NNGNRNLDIN NSPTIVITNS SLNKSALSTN NNNNNNNNNN NNPTTKLSSS
TSSTSTTTTT NTNTNTTTNE KIAHAIISPS SSTTSFKFDD DEDEDEDLLE INNKLHSQLT
EELKKKQQQY KQLIFDIIDM IKDKKKLMNS LDLTNSDSQL DIKSIAKLSL SLKRALDNFT
SESGFDIEDE QDPIINKEND SFVNLKIMTS HFISRIVLIL TTILSISNEF NSTLYQLLLP
FNENNNNNNE NNNINNQ
