MGR2_YEAST
ID MGR2_YEAST Reviewed; 113 AA.
AC Q02889; D6W3R8;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Protein MGR2;
DE AltName: Full=Mitochondrial genome-required protein 2;
GN Name=MGR2; OrderedLocusNames=YPL098C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION.
RX PubMed=16267274; DOI=10.1091/mbc.e05-06-0585;
RA Dunn C.D., Lee M.S., Spencer F.A., Jensen R.E.;
RT "A genomewide screen for petite-negative yeast strains yields a new subunit
RT of the i-AAA protease complex.";
RL Mol. Biol. Cell 17:213-226(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Required for cell viability in cells lacking mitochondrial
CC DNA. {ECO:0000269|PubMed:16267274}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the MGR2 family. {ECO:0000305}.
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DR EMBL; U43281; AAB68201.1; -; Genomic_DNA.
DR EMBL; AY558335; AAS56661.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11334.1; -; Genomic_DNA.
DR PIR; S61968; S61968.
DR RefSeq; NP_015227.1; NM_001183912.1.
DR AlphaFoldDB; Q02889; -.
DR BioGRID; 36082; 264.
DR ComplexPortal; CPX-6127; TIM23 mitochondrial inner membrane pre-sequence translocase complex, sort variant.
DR DIP; DIP-4556N; -.
DR IntAct; Q02889; 1.
DR STRING; 4932.YPL098C; -.
DR TCDB; 1.A.111.1.3; the reactive oxygen species modulator 1 (romo1) family.
DR MaxQB; Q02889; -.
DR PaxDb; Q02889; -.
DR PRIDE; Q02889; -.
DR EnsemblFungi; YPL098C_mRNA; YPL098C; YPL098C.
DR GeneID; 856006; -.
DR KEGG; sce:YPL098C; -.
DR SGD; S000006019; MGR2.
DR VEuPathDB; FungiDB:YPL098C; -.
DR eggNOG; KOG4096; Eukaryota.
DR HOGENOM; CLU_142435_0_0_1; -.
DR InParanoid; Q02889; -.
DR OMA; FMIGFCV; -.
DR BioCyc; YEAST:G3O-34001-MON; -.
DR PRO; PR:Q02889; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q02889; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IC:ComplexPortal.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IPI:SGD.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IMP:SGD.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IMP:SGD.
DR InterPro; IPR018450; Romo1/Mgr2.
DR PANTHER; PTHR28525; PTHR28525; 1.
DR Pfam; PF10247; Romo1; 1.
DR SMART; SM01378; Romo1; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..113
FT /note="Protein MGR2"
FT /id="PRO_0000252278"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 44..56
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..113
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 113 AA; 12252 MW; 43BA58C679D86E93 CRC64;
MPPLPQNYAQ QQPSNWDKFK MGLMMGTTVG VCTGILFGGF AIATQGPGPD GVVRTLGKYI
AGSAGTFGLF MSIGSIIRSD SESSPMSHPN LNLQQQARLE MWKLRAKYGI RKD
